FastQCFastQC Report
Mon 6 Jun 2016
ERR523067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8035428
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223120.2776703369129808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177190.22051096718183524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109140.13582350560542636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120450.054.7398341
GTATCAA231350.044.5702441
TCAACGC283700.035.7996834
ATCAACG289100.035.084413
CAACGCA290600.034.8668335
TATCAAC297500.034.367092
AACGCAG299950.033.7857326
ACGCAGA345100.029.3113967
GTACATG337700.028.8755861
TACATGG333600.028.618992
CGCAGAG355050.028.3689278
ACATGGG343350.027.2138613
CATGGGG227150.025.8010564
GCAGAGT395350.024.9899
ATGGGGG135550.022.8825155
GAGTACT218450.021.73310312-13
CAGAGTA386600.020.99137310-11
AGAGTAC365200.020.388410-11
GTACTTT238150.019.97614514-15
GTGGTAT57400.019.759561
Produced by FastQC (version 0.11.5)