FastQCFastQC Report
Mon 6 Jun 2016
ERR523067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8035428
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202890.25249432886462303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169800.21131419508705696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103300.12855569112186682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.054.282981
GTATCAA221050.041.8381161
TCAACGC278150.032.70824
ATCAACG281450.032.32553
CAACGCA284350.032.011555
TATCAAC291750.031.339462
AACGCAG294500.030.9399876
TACATGG328850.027.7609162
GTACATG332050.027.710511
ACGCAGA338850.026.8625377
ACATGGG340150.026.3187753
CGCAGAG351950.025.8150588
CATGGGG215900.025.3138034
ATGGGGG131700.023.0503275
GCAGAGT395200.022.749
GAGTACT215650.020.09622412-13
AGAGTAC365000.020.00559410-11
CAGAGTA385300.019.48834210-11
GTGGTAT57650.019.3387411
AGTACTT231850.018.27644212-13
Produced by FastQC (version 0.11.5)