FastQCFastQC Report
Mon 6 Jun 2016
ERR523066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9923500
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT418740.42196805562553535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296440.2987252481483348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251990.2539325842696629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144280.14539225071799264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG136300.041.9501882
GTACCTG154100.037.877161
ACCTGGG161550.034.9570243
CCTGGGG154200.025.7345714
TATAACG8000.024.0985052
GTACATG451600.022.9543971
TACATGG457750.022.1676332
CATGGGG270400.021.7003524
ACATGGG469150.020.8172423
ATGGGGG158350.020.3384675
GAGTACT261300.019.74520512-13
ATAACGC9400.019.0087763
TGGGGGG158550.018.849396
CTGGGGG106700.018.7270875
GTACTTT298650.017.1892814-15
GTACCGG15750.017.0216851
AGTACTT277350.016.89140312-13
GTATAGC27950.016.6594871
GTACCCG18800.016.5117971
GTATAAC25050.015.7717461
Produced by FastQC (version 0.11.5)