FastQCFastQC Report
Mon 6 Jun 2016
ERR523066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9923500
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT439200.4425857812263818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264160.26619640247896403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241890.24375472363581396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139670.14074671234947347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG139550.041.7939952
GTACCTG157300.037.9276471
ACCTGGG161450.035.5978053
CCTGGGG146650.025.8919094
GTACATG447350.021.26041
TACATGG453100.020.6928732
CATGGGG267150.020.5809524
ATGGGGG156450.019.824545
GAGTACT270100.019.73534212-13
ACATGGG462200.019.7041913
GAGTACG13900.019.6171631
TGGGGGG147700.019.5989486
CTGGGGG99300.018.7878045
TATAACG8100.018.5667952
GTACTTT299700.017.61173614-15
AGTACTT283400.017.0841612-13
GTATAGG24500.016.5027141
GTATAGA33500.015.7179611
AGAGTAC535500.015.25593710-11
GTATTAG24950.015.0744841
Produced by FastQC (version 0.11.5)