FastQCFastQC Report
Mon 6 Jun 2016
ERR523065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10147368
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281480.2773921276926194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223030.2197909842236923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129700.12781639534507866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186800.042.47191
GTATCAA312650.037.1004751
TCAACGC373500.030.475044
ATCAACG382450.029.8007973
CAACGCA382900.029.6772145
TATCAAC391500.029.2610322
AACGCAG393850.028.868426
GTACATG388000.027.7609961
TACATGG399850.026.4868282
ACATGGG409700.025.398173
ACGCAGA448550.025.2849887
CGCAGAG465750.024.3095518
CATGGGG274250.023.2366644
GCAGAGT515450.021.74599
GAGTACT251150.021.14800612-13
GTACTTT268600.019.53008314-15
AGAGTAC434400.019.44970510-11
CAGAGTA495400.019.03197110-11
ATGGGGG143500.018.7652745
AGTACTT273000.017.63978412-13
Produced by FastQC (version 0.11.5)