FastQCFastQC Report
Mon 6 Jun 2016
ERR523065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10147368
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252490.24882314310469472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208520.20549170977144024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.12249481836078084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181150.044.4288181
GTATCAA305850.036.8494831
TCAACGC365850.030.2111844
CAACGCA372100.029.7037375
ATCAACG375750.029.3779723
AACGCAG386200.028.6922766
TATCAAC389800.028.5202792
GTACATG376400.026.3310411
ACGCAGA440150.025.1220287
TACATGG388800.024.940812
CGCAGAG461500.023.9249928
ACATGGG398250.023.88323
GCAGAGT508500.021.4545979
CATGGGG272350.021.2577274
GAGTACT254250.020.55887212-13
AGAGTAC432100.019.7498710-11
GTACTTT266500.019.34689914-15
CAGAGTA491000.018.98425110-11
TATACCG8600.018.578645
AGTACTT270950.018.19008412-13
Produced by FastQC (version 0.11.5)