##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523064_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1435762
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.403855931554116	33.0	31.0	34.0	28.0	34.0
2	31.662215603978932	33.0	31.0	34.0	28.0	35.0
3	31.621651081446647	33.0	31.0	34.0	27.0	36.0
4	33.852215757207674	35.0	33.0	37.0	31.0	37.0
5	33.96416397703798	35.0	34.0	37.0	31.0	37.0
6	34.34620013623428	35.0	35.0	37.0	31.0	37.0
7	34.26382297344546	35.0	34.0	37.0	31.0	37.0
8	34.462941629601566	36.0	34.0	37.0	31.0	37.0
9	35.486639150499876	37.0	35.0	39.0	31.0	39.0
10-11	35.52272591139757	37.0	34.5	39.0	31.0	39.0
12-13	35.62203171556288	37.5	35.0	39.0	31.0	39.0
14-15	36.14051562863483	38.0	35.0	40.0	30.5	41.0
16-17	36.334349634549454	38.0	36.0	40.0	30.5	41.0
18-19	36.34490012968723	38.0	35.5	40.0	30.5	41.0
20-21	36.55820045383567	38.5	36.0	40.0	31.0	41.0
22-23	36.45657810974242	38.0	35.5	40.0	31.0	41.0
24-25	36.40521897083221	38.0	35.5	40.0	31.0	41.0
26-27	36.15678016272892	37.5	36.0	40.0	30.0	41.0
28-29	35.99878496575337	38.0	35.0	40.0	30.0	41.0
30-31	35.786680174012126	37.0	35.5	40.0	29.5	41.0
32-33	35.53825042033429	37.5	34.5	40.0	28.0	41.0
34-35	35.35171114711213	37.5	34.5	40.0	28.0	41.0
36-37	35.13564713371715	37.5	35.0	40.0	27.5	41.0
38-39	34.86539308046876	36.5	35.0	40.0	26.5	41.0
40-41	34.78362883263382	37.0	34.0	40.0	24.5	41.0
42-43	34.411259665599175	36.0	35.0	40.0	23.5	41.0
44-45	34.11268476251635	37.0	33.5	40.0	21.5	41.0
46-47	33.56200226778533	36.5	33.0	40.0	19.5	41.0
48-49	33.149502145898836	37.0	33.0	39.0	14.0	41.0
50-51	32.06474471395677	36.0	31.5	38.0	11.5	39.5
52-53	32.27795205612072	36.5	31.5	38.5	10.0	39.5
54-55	33.03583741595055	37.5	33.0	39.5	10.0	40.5
56-57	33.03669062142611	37.0	32.5	40.0	10.0	41.0
58-59	32.971359459297574	37.0	33.0	40.0	10.0	41.0
60-61	32.525002402905216	36.5	32.0	39.5	9.5	41.0
62-63	32.30725774884695	37.0	31.0	39.0	9.0	41.0
64-65	31.880319300831196	36.0	31.0	39.0	9.0	40.5
66-67	31.49777156659669	36.0	30.5	38.0	9.0	40.0
68-69	31.03985235714554	35.0	30.0	37.5	8.5	39.5
70-71	30.52362369250614	35.0	29.0	37.0	8.0	39.0
72-73	29.970018707835976	35.0	28.0	37.0	8.0	38.5
74-75	29.474932126633803	34.5	26.5	36.0	7.5	37.0
76-77	29.11728719662451	34.0	26.0	36.5	7.0	37.5
78-79	28.662799614420777	34.0	25.5	36.0	7.0	37.0
80-81	28.25589930643101	34.0	24.5	35.5	7.0	37.5
82-83	27.798419236614425	34.0	23.0	35.0	7.0	37.0
84-85	27.42543193091891	33.5	20.0	35.0	7.0	36.5
86-87	27.13307741812362	33.0	19.5	35.0	7.0	37.0
88-89	26.789928971514776	33.0	18.0	35.0	7.0	36.5
90-91	26.408923972078938	33.0	17.0	35.0	7.0	36.0
92-93	25.955136714859428	32.0	17.0	35.0	7.0	35.0
94-95	25.699441481248286	32.0	16.0	35.0	7.0	36.0
96-97	25.391325999713043	31.0	15.0	35.0	7.0	35.0
98-99	25.20372422448846	31.0	12.0	35.0	7.0	35.0
100	23.870112177366444	29.0	10.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12051350895993806
1101	2	-0.22956906863304383
1101	3	-0.16679124556533864
1101	4	-0.15150811231244887
1101	5	-0.09073082104927721
1101	6	-0.04436532116488223
1101	7	-0.22598005977177849
1101	8	-0.06876529339500337
1101	9	-0.1635530870715698
1101	10-11	-0.19469711320958538
1101	12-13	-0.1694834148388864
1101	14-15	-0.24495005926033286
1101	16-17	-0.1726319212921439
1101	18-19	-0.22568373740062242
1101	20-21	-0.14227747541143998
1101	22-23	-0.21060405262939952
1101	24-25	-0.19435412938803864
1101	26-27	-0.3028915222731072
1101	28-29	-0.2923084997814982
1101	30-31	-0.3497223250863115
1101	32-33	-0.2713885048224043
1101	34-35	-0.35687139040404503
1101	36-37	-0.49505880613926223
1101	38-39	-0.4695598984917737
1101	40-41	-0.3883166339680102
1101	42-43	-0.414805381723518
1101	44-45	-0.33839849505893227
1101	46-47	-0.3940079527458167
1101	48-49	-0.6201540269907255
1101	50-51	-0.39335322744702594
1101	52-53	-0.35653897551897984
1101	54-55	-0.23155666656917617
1101	56-57	-0.3593253995447512
1101	58-59	-0.09683537450356283
1101	60-61	-0.16240100362482224
1101	62-63	-0.2452270015349285
1101	64-65	-0.14654970666591183
1101	66-67	-0.21240985681500035
1101	68-69	-0.1499406296866006
1101	70-71	-0.31315895802759286
1101	72-73	-0.44459016729506473
1101	74-75	-0.4519297722782909
1101	76-77	-0.3688754149961575
1101	78-79	-0.18001343852373353
1101	80-81	-0.26789805327171123
1101	82-83	-0.6382832425760014
1101	84-85	-0.47782380843861816
1101	86-87	-0.453780030164296
1101	88-89	-0.7180436371678525
1101	90-91	-0.48930774903995555
1101	92-93	-0.6226643904501614
1101	94-95	-0.5367928465164269
1101	96-97	-0.6531255514527139
1101	98-99	-0.6754621606723319
1101	100	-0.7426937140353544
1102	1	0.013818322958822904
1102	2	-0.005531575725203908
1102	3	0.06089711634890449
1102	4	0.06267562377824021
1102	5	0.1017873869796233
1102	6	0.06475721973804838
1102	7	-0.01470266088568195
1102	8	0.11245293663406386
1102	9	-0.20311516805532648
1102	10-11	-0.11016380612205978
1102	12-13	-0.01545014827797786
1102	14-15	-0.048555480563436504
1102	16-17	-0.06301987678234156
1102	18-19	-0.18423861622603965
1102	20-21	-0.08803897049614307
1102	22-23	-5.971631049490611E-4
1102	24-25	-0.11640871294812172
1102	26-27	-0.09192733622121096
1102	28-29	-0.17784020896252173
1102	30-31	-0.008221335079014125
1102	32-33	0.056887203524922825
1102	34-35	0.011751746819065545
1102	36-37	-0.5249373420863748
1102	38-39	-0.06747972526943613
1102	40-41	-0.8971204619962165
1102	42-43	-0.7926535342888528
1102	44-45	-0.17738220335993304
1102	46-47	-0.15896777350239688
1102	48-49	-0.11132356718733405
1102	50-51	-0.024065164271824813
1102	52-53	0.12429135691504456
1102	54-55	0.11303100407681654
1102	56-57	0.3073721445424198
1102	58-59	0.10258712124114311
1102	60-61	0.07365436730949426
1102	62-63	-0.31625394594399836
1102	64-65	0.03612024458636043
1102	66-67	0.2065777304619587
1102	68-69	0.054136637198524795
1102	70-71	-0.08842556525522483
1102	72-73	-0.09833627384532306
1102	74-75	-0.21379625445236883
1102	76-77	-0.31735277219773295
1102	78-79	-0.27528629842901253
1102	80-81	-0.3617964819969792
1102	82-83	-0.5336388925650013
1102	84-85	-0.6976511650612451
1102	86-87	-0.7643120288211271
1102	88-89	-0.7066455268659908
1102	90-91	-0.7308078706198025
1102	92-93	-0.7247709059718552
1102	94-95	-0.9530399662320441
1102	96-97	-0.8377755172945633
1102	98-99	-0.8083544293522067
1102	100	-0.8028352098147948
1103	1	0.08433819926911923
1103	2	0.1537898054020701
1103	3	0.09910863913884427
1103	4	0.1612684859445963
1103	5	0.11915920403495761
1103	6	0.1427782961063997
1103	7	-0.030556264966996594
1103	8	-0.09039761985797412
1103	9	0.14309432300842673
1103	10-11	0.12640782338932155
1103	12-13	0.140779827497596
1103	14-15	0.12819437152244006
1103	16-17	0.07225708545210807
1103	18-19	0.1998555911216613
1103	20-21	0.011771252882105898
1103	22-23	-0.06833876548943607
1103	24-25	0.05854523831278158
1103	26-27	0.09825116543655099
1103	28-29	-0.12471642995181043
1103	30-31	-0.10293270716399405
1103	32-33	-0.11156047952579229
1103	34-35	-0.2039706881266241
1103	36-37	-0.2019352991968546
1103	38-39	-0.12550169470424066
1103	40-41	-0.07500562481340012
1103	42-43	-0.1970027679262003
1103	44-45	-0.2246148136815478
1103	46-47	-0.1004727579295448
1103	48-49	-0.27766975323540066
1103	50-51	-0.22026879458696413
1103	52-53	-0.021042370855973047
1103	54-55	-0.022024366043410737
1103	56-57	0.1484398054897369
1103	58-59	-0.14894420864644076
1103	60-61	-0.049570384027312286
1103	62-63	-0.08595108478233726
1103	64-65	0.05607441911387312
1103	66-67	0.005399719990752061
1103	68-69	-0.28378212724403795
1103	70-71	-0.19932711584185725
1103	72-73	-0.14580080236205006
1103	74-75	0.027571421007280605
1103	76-77	-0.09050217105237834
1103	78-79	-0.12526620443377112
1103	80-81	-0.20316499470643024
1103	82-83	-0.11691957218857141
1103	84-85	-0.38008957138415767
1103	86-87	-0.6854578888999399
1103	88-89	-0.1998344997279311
1103	90-91	-0.5517505580800339
1103	92-93	-0.293180633698217
1103	94-95	-0.5602799443519153
1103	96-97	-0.420026658745126
1103	98-99	-0.3438686123638526
1103	100	-0.19394250058606843
1104	1	0.03775162414634892
1104	2	-0.004751666107249974
1104	3	-0.06963149528862544
1104	4	-0.08273687047476841
1104	5	-0.10206057830290405
1104	6	0.1586283972838345
1104	7	0.0096483489038377
1104	8	0.027078344530721665
1104	9	0.08587526669802514
1104	10-11	-0.043755987962668996
1104	12-13	0.1728582159863521
1104	14-15	-0.19059630947671735
1104	16-17	-0.1211747261269096
1104	18-19	-0.20294495306177396
1104	20-21	-0.06299103212638357
1104	22-23	-0.13561712340861476
1104	24-25	-0.04673155403943241
1104	26-27	-0.19312495444115285
1104	28-29	-0.16914037604595933
1104	30-31	-0.15614568777085225
1104	32-33	-0.016849578380721653
1104	34-35	0.07278654278663765
1104	36-37	-0.07898603177093122
1104	38-39	0.09313175526926187
1104	40-41	0.09123612097921097
1104	42-43	-0.32183818952506016
1104	44-45	-0.2096511740586564
1104	46-47	-7.232149278735278E-4
1104	48-49	-0.16745771612708182
1104	50-51	-0.39744815540182543
1104	52-53	-0.41134619536861194
1104	54-55	-0.1467038490803816
1104	56-57	-0.10245325886877055
1104	58-59	-0.24744455733068804
1104	60-61	-0.28078175411194906
1104	62-63	-0.05686643571154093
1104	64-65	-0.004047904151544657
1104	66-67	-0.19006013580400705
1104	68-69	-0.2744825695476081
1104	70-71	-0.2062513499306462
1104	72-73	-0.17262644956327833
1104	74-75	-0.230230705039407
1104	76-77	-0.29226053989568257
1104	78-79	-0.41936548925176353
1104	80-81	-0.49570264614244053
1104	82-83	-0.5054937430309252
1104	84-85	-0.47057411604636457
1104	86-87	-0.6341212285026572
1104	88-89	-0.5790579762643766
1104	90-91	-0.7314097634845531
1104	92-93	-0.9305495820878917
1104	94-95	-0.9230922218584965
1104	96-97	-0.959796049494571
1104	98-99	-1.1084651641829346
1104	100	-0.9589068426349172
1105	1	0.030394034708443485
1105	2	0.11652444373696014
1105	3	-0.06415274447911301
1105	4	0.09008373739517594
1105	5	0.04844068436932503
1105	6	0.04006022015447286
1105	7	-0.13650444491706537
1105	8	-0.05430030158014887
1105	9	0.006526053151411304
1105	10-11	-0.10245893839023523
1105	12-13	0.12384292380653505
1105	14-15	0.08741609328538402
1105	16-17	-0.12531468029781223
1105	18-19	-0.09203279511135065
1105	20-21	0.08589203526798883
1105	22-23	-0.20616459028622813
1105	24-25	-0.042398806533064715
1105	26-27	-0.041611733617479274
1105	28-29	-0.1180220092123676
1105	30-31	3.054144333347608E-4
1105	32-33	-0.09479655828277345
1105	34-35	-0.12181171341281782
1105	36-37	-0.27757613052304464
1105	38-39	-0.21709837212327443
1105	40-41	-0.3064904622153861
1105	42-43	-0.19230396057152888
1105	44-45	-0.20006942802954342
1105	46-47	-0.07185871526933596
1105	48-49	0.041247826943340726
1105	50-51	-0.30217735661408085
1105	52-53	-0.14348978144600721
1105	54-55	-0.03356256990349493
1105	56-57	-0.11325051309450629
1105	58-59	-0.05342302625923878
1105	60-61	-0.04993035226444675
1105	62-63	-0.19575183096822002
1105	64-65	-0.243354079826819
1105	66-67	-0.2279670566158707
1105	68-69	-0.14592637373798567
1105	70-71	-0.2657240946347592
1105	72-73	-0.19828202964780672
1105	74-75	-0.26129718591838724
1105	76-77	-0.13589990437824184
1105	78-79	-0.38130466474720137
1105	80-81	-0.23660964087220648
1105	82-83	-0.28789295266099657
1105	84-85	-0.3252225591919107
1105	86-87	-0.3497049978964526
1105	88-89	-0.4458377814520915
1105	90-91	-0.4124749756234145
1105	92-93	-0.6182599585065383
1105	94-95	-0.5077740052878674
1105	96-97	-0.42347422639224774
1105	98-99	-0.4588404354450901
1105	100	-0.21374322480592056
1106	1	-0.00860645618453404
1106	2	-0.11998715600038068
1106	3	-0.1715509904398722
1106	4	0.21718995516039996
1106	5	0.10094391980032213
1106	6	0.007305333006726755
1106	7	0.04661988701158748
1106	8	-1.011312102137346
1106	9	0.163491739519678
1106	10-11	0.04602449981568668
1106	12-13	-0.03774586938300928
1106	14-15	0.02314948952102469
1106	16-17	-0.09385502808804347
1106	18-19	-0.03154351719154391
1106	20-21	-0.08749465876967832
1106	22-23	0.006267752548332339
1106	24-25	0.04148704703631978
1106	26-27	0.0019502368396828729
1106	28-29	-0.10647327373993676
1106	30-31	0.049164672653667196
1106	32-33	-0.23507825860004772
1106	34-35	-0.17899171388032897
1106	36-37	-0.1135424352889558
1106	38-39	-0.19515322463225004
1106	40-41	-0.1817409557647096
1106	42-43	-0.09937643210080438
1106	44-45	-0.0998858213049445
1106	46-47	-0.12308477892391778
1106	48-49	-0.07610800246391847
1106	50-51	0.056281834453791646
1106	52-53	0.12093152798444606
1106	54-55	0.14484462050985059
1106	56-57	0.06055269619803738
1106	58-59	0.035411602309025625
1106	60-61	-0.042104753151882335
1106	62-63	-0.1160433491450732
1106	64-65	-0.1332012017276547
1106	66-67	-0.016902732635003304
1106	68-69	0.06619411392487962
1106	70-71	0.27145520455707484
1106	72-73	0.17405232584796337
1106	74-75	0.2877087026722833
1106	76-77	0.16297316684799057
1106	78-79	0.22284964990457112
1106	80-81	0.04756373572771011
1106	82-83	0.2509832211788705
1106	84-85	0.05877581724862324
1106	86-87	-0.011773527406347739
1106	88-89	-0.1096765007951852
1106	90-91	-0.18420495898405775
1106	92-93	0.12327054379811031
1106	94-95	-0.0045465433320721615
1106	96-97	-0.10834218468122359
1106	98-99	0.018530142002308736
1106	100	-0.059169313536418855
1107	1	-0.05784752439864249
1107	2	0.13436477294490246
1107	3	-0.1546427386856628
1107	4	0.25626984607948344
1107	5	0.24996015314180653
1107	6	0.294532136325401
1107	7	0.2723974029158427
1107	8	0.2793237333163816
1107	9	0.3290638109110873
1107	10-11	0.24856654353121144
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2316	96-97	0.654972942414421
2316	98-99	0.5372217986670442
2316	100	0.3937883224610914
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2422.0
1	216.0
2	160.0
3	125.0
4	82.0
5	89.0
6	113.0
7	375.0
8	1232.0
9	2698.0
10	4822.0
11	6258.0
12	6274.0
13	5545.0
14	5145.0
15	6446.0
16	9658.0
17	13897.0
18	17819.0
19	22752.0
20	27859.0
21	32034.0
22	29340.0
23	25303.0
24	22940.0
25	22075.0
26	23063.0
27	25316.0
28	29467.0
29	34903.0
30	41911.0
31	51409.0
32	64512.0
33	82767.0
34	112618.0
35	175175.0
36	191344.0
37	191112.0
38	134736.0
39	11735.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.099993094929374	17.124993635477832	13.116356019861493	16.658657249731295
2	14.942078510608194	25.38767078318942	33.82648434924487	25.843766356957516
3	28.225645338627274	28.85781664098398	29.406094271061082	13.510443749327663
4	13.925374333325182	18.72382635544115	35.11094656472552	32.23985274650814
5	10.121123763631577	41.62447930892602	32.95049776075769	15.303899166684712
6	26.257845277751983	32.815656133682346	16.591736947461687	24.334761641103984
7	20.223988716777857	38.48374720275936	22.123563850399556	19.168700230063223
8	26.347040316600733	26.02166994597176	21.25289935721896	26.378390380208543
9	25.566063954438363	20.22391175924284	23.149372731449734	31.06065155486906
10-11	24.918570925413377	26.339456352933908	22.854525836866785	25.88744688478593
12-13	32.13483781508029	25.455610527183026	23.6678689673759	18.74168269036079
14-15	27.965104652189527	25.343660751773058	29.106783938451507	17.584450657585908
16-17	19.018880068355255	29.828043805444405	31.043981358672433	20.109094767527903
18-19	19.528280085706324	30.6108635352254	31.836241991212937	18.02461438785534
20-21	22.41780706430387	25.47323840114279	29.320166963918854	22.788787570634486
22-23	19.487587612744687	27.35165172712295	29.097833068051713	24.062927592080648
24-25	23.791464198312582	28.142440915775374	26.66690169925068	21.399193186661368
26-27	22.651695731377636	23.443024242485592	32.33389029885208	21.57138972728469
28-29	19.561526944383257	28.896298046898316	31.561849499916743	19.980325508801684
30-31	22.20717091981336	26.61203288218984	29.779170517721365	21.401625680275433
32-33	18.92150685337974	27.737328709141973	32.835412415849085	20.505752021629206
34-35	19.356579368963047	26.804166890629244	31.717393899329227	22.121859841078482
36-37	20.733741219441704	26.11234367429801	32.81178224913538	20.342132857124906
38-39	21.976486850648218	25.68839492037783	31.718840539238613	20.616277689735337
40-41	18.764331539301583	28.3858043397507	30.806525266800545	22.043338854147173
42-43	19.749755997436402	28.07034813824997	28.70751248636867	23.47238337794496
44-45	22.07696928258078	24.782478342071197	29.59376528078958	23.54678709455844
46-47	21.242560861238573	28.444690782082056	31.196548232634097	19.11620012404527
48-49	20.695140294114463	26.93947891006816	31.69607124818007	20.669309547637308
50-51	16.512999038762917	28.335552020238335	33.51363218475856	21.637816756240188
52-53	18.128362220942208	30.047337278106507	27.54209212616292	24.28220837478836
54-55	17.032371406915576	27.302710936438977	28.409443606733543	27.2554740499119
56-57	21.071730057974193	24.91511246071573	27.431051348669765	26.582106132640316
58-59	23.382474769179314	23.6211590834271	28.808746381944477	24.18761976544911
60-61	20.85449262209967	25.610485257288456	31.049974258065077	22.485047862546796
62-63	17.868492442836843	25.85234116690001	31.43458663417325	24.844579756089896
64-65	16.83860458328882	29.237161212481748	29.74093058037164	24.183303623857793
66-67	20.51278201893824	25.965258716218997	26.48274003249707	27.03921923234569
68-69	20.346941860842684	25.43592060503242	27.62214872258792	26.594988811536986
70-71	22.427348027516498	25.731723245348437	26.45448875236341	25.38643997477166
72-73	22.373651612128103	22.97995902535385	30.632704138048094	24.013685224469953
74-75	21.48293514166591	27.10724985857852	27.576175544210795	23.83363945554477
76-77	21.148419184734674	24.49214393509275	28.808073739953393	25.551363140219184
78-79	22.243815575845392	24.682599814947366	29.97363872837416	23.099945880833086
80-81	23.157531300707426	24.38882279177454	28.29509242890138	24.158553478616653
82-83	21.378407394799552	23.950212539387433	29.412378782721028	25.25900128309199
84-85	21.87527279710879	25.47298217434572	27.801805262147113	24.84993976639838
86-87	22.911603095387818	24.744457320750012	27.007653274106207	25.336286309755963
88-89	23.466672626172755	23.780943454995718	26.510385603323172	26.241998315508358
90-91	25.61405812065131	22.14128862381496	26.597598622037687	25.647054633496037
92-93	25.448627158826735	23.689238568791826	27.564278235940375	23.29785603644106
94-95	24.23001058345901	23.051164791544963	27.877026561304625	24.841798063691403
96-97	24.014183357077012	22.928713092544484	27.992995614402638	25.064107935975866
98-99	26.56586085558208	23.26085188137215	24.922538306662073	25.2507489563837
100	26.2195381723986	20.453432767648184	25.778076074514278	27.548952985438945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3928.0
1	2970.0
2	1867.5
3	1412.5
4	990.0
5	948.0
6	1313.5
7	2063.5
8	2938.0
9	3436.0
10	2908.0
11	2211.5
12	2089.0
13	2091.5
14	2178.0
15	2307.5
16	2402.5
17	2571.5
18	2840.0
19	3099.5
20	3484.0
21	3931.0
22	4488.5
23	5101.0
24	5965.0
25	7043.0
26	8207.0
27	9647.5
28	11445.5
29	13239.0
30	14744.0
31	16421.5
32	17748.0
33	18470.5
34	18605.0
35	17789.5
36	17460.0
37	17197.0
38	16237.0
39	15262.0
40	14643.0
41	14182.5
42	13502.0
43	13181.5
44	13978.5
45	17441.5
46	33661.5
47	79297.0
48	158424.0
49	186330.5
50	185951.0
51	159250.5
52	85608.0
53	49141.0
54	34649.5
55	23739.5
56	15760.0
57	10827.0
58	7669.0
59	5516.5
60	4344.5
61	3588.5
62	2886.5
63	2397.5
64	2165.5
65	1886.0
66	1559.0
67	1293.0
68	1103.0
69	857.5
70	675.0
71	576.0
72	504.0
73	435.5
74	344.5
75	271.5
76	222.5
77	187.0
78	147.0
79	104.5
80	83.5
81	65.5
82	53.0
83	44.5
84	34.5
85	30.0
86	21.5
87	12.5
88	9.5
89	5.5
90	5.0
91	3.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14159728422955895
2	0.06790819091186423
3	0.2916917985014229
4	0.2937116318721348
5	0.29607971237572805
6	0.2459321252408129
7	0.2470465160660332
8	0.2471161654926095
9	0.25630988980067726
10-11	0.23544988654108412
12-13	0.24433018842955867
14-15	0.2183857770298977
16-17	0.22608203866657564
18-19	0.23882788373003325
20-21	0.2421362314924061
22-23	0.23137539508637223
24-25	0.2323853117717282
26-27	0.2296689841352536
28-29	0.24032534640142308
30-31	0.22663923407918582
32-33	0.2395592027090841
34-35	0.2250372972679316
36-37	0.23235048705844008
38-39	0.2348578664151858
40-41	0.2511558322340332
42-43	0.2368080503593214
44-45	0.22312193803708416
46-47	0.22430597828888074
48-49	0.2340568980095587
50-51	0.22517659612108412
52-53	0.24286755047145694
54-55	0.2520264500662366
56-57	0.24885740115701627
58-59	0.2462455476604061
60-61	0.22806704732399938
62-63	0.2561009415209485
64-65	0.2478823091849485
66-67	0.2530363667515925
68-69	0.25815559960494844
70-71	0.28131403394155857
72-73	0.2886968731586433
74-75	0.2696825797033213
76-77	0.2654339646821688
78-79	0.26027990711552473
80-81	0.24558387810793156
82-83	0.2559268179545078
84-85	0.2672448497731518
86-87	0.2619166686400671
88-89	0.2699611774096264
90-91	0.26414545029050773
92-93	0.24596694995410104
94-95	0.26539913996888065
96-97	0.26480711984298233
98-99	0.2509120592410163
100	0.27365259701816874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1435762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.774939785344117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74554104713866	16.15158933833305
2	8.338750034988509	3.4647405972384493
3	3.5507319576446768	2.2129872784189564
4	2.071048926881329	1.7210366699384465
5	1.3444956465982503	1.3965908049867979
6	1.0207725907350051	1.2723893464229667
7	0.7643666866853331	1.1115770322867553
8	0.5930948403101763	0.9857207675553759
9	0.5140630579539103	0.9611666167376116
>10	3.536948828438803	14.345043247444478
>50	0.296766510740938	4.225903158306254
>100	0.17271944709976236	6.932247467178189
>500	0.018590155754377186	2.733940994784473
>1k	0.01994216708196826	10.472453179230666
>5k	0.005408045310364273	8.24276117719154
>10k+	0.006760056637955341	23.76985232394598
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	47632	3.3175414866809403	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	30015	2.090527538686774	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	29307	2.041215744670774	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	26214	1.8257900682703678	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	19917	1.3872076291195894	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	15637	1.0891080833731497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13754	0.9579582131300313	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	13436	0.9358096954787771	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13332	0.9285661551148449	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	13311	0.9271035171567432	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	13157	0.9163775054639975	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	13059	0.9095518616595231	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	11916	0.8299425670828453	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11905	0.8291764233905062	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	11705	0.815246538075252	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	11158	0.7771483017380317	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	11097	0.7728996867168793	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	10930	0.761268232478642	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	10781	0.7508904679187777	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	10243	0.7134190764207438	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	9645	0.6717687193281338	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	9424	0.6563761960547779	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9347	0.6510131902084051	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	8668	0.603721229563117	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	8431	0.5872143154645407	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	8182	0.5698716082470493	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7947	0.5535039930016256	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	7928	0.5521806538966765	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7372	0.5134555727202698	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	6589	0.4589200717110496	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	6368	0.4435275484376937	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	6177	0.4302245079616259	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5887	0.4100261742545074	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5234	0.3645450987002024	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	5185	0.3611322767979651	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	5001	0.3483167823079313	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4797	0.334108299286372	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	4704	0.3276309026147788	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	4657	0.32435737956569405	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	4640	0.3231733393138974	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4456	0.3103578448238635	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4364	0.3039500975788466	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	4330	0.30158201707525345	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	4322	0.30102482166264327	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	4244	0.2955921663896941	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	4135	0.2880003788928806	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	4087	0.2846572064172196	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	4008	0.2791549017176942	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	3802	0.26480711984298233	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3763	0.26209079220650777	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3624	0.2524095219124061	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	3572	0.24878775173044002	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	3478	0.24224070563227054	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	3406	0.237225946918779	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3381	0.23548471125437223	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	3354	0.2336041767368129	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3191	0.22225132020488075	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	3087	0.2150077798409486	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	3043	0.21194320507159264	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	2835	0.19745612434372825	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2678	0.18652116437125374	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	2473	0.17224303192311816	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	2443	0.17015354912583006	No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG	2382	0.16590493410467752	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2274	0.15838279603444025	No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT	2226	0.15503962355877923	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	2107	0.146751341796203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2031	0.14145798537640641	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1973	0.1374183186349827	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	1887	0.13142846794942337	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	1846	0.12857284145979625	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1773	0.12348843331972847	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	1700	0.1184040251796607	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	1613	0.11234452506752513	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1606	0.11185697908149123	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	1581	0.11011574341708444	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	1544	0.10753871463376243	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	1511	0.10524028355674549	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.741235664406775E-4	0.0
12-13	0.0	0.0	0.0	4.527212727457615E-4	0.0
14-15	0.0	0.0	0.0	8.009684056271165E-4	0.0
16-17	0.0	0.0	0.0	9.05442545491523E-4	0.0
18-19	0.0	0.0	0.0	0.0014278132448135555	0.0
20-21	0.0	0.0	0.0	0.007139066224067778	0.0
22-23	0.0	0.0	0.0	0.02420317573525417	0.0
24-25	0.0	0.0	0.0	0.04669994051938971	0.0
26-27	0.0	0.0	0.0	0.0905442545491523	0.0
28-29	0.0	0.0	0.0	0.23311663075077904	0.0
30-31	0.0	0.0	0.0	0.5192712998393885	0.0
32-33	0.0	0.0	0.0	1.057069347148065	0.0
34-35	0.0	0.0	0.0	1.9719842146539608	0.0
36-37	0.0	0.0	0.0	3.152263397415449	0.0
38-39	0.0	0.0	0.0	4.461951214755649	0.0
40-41	0.0	0.0	0.0	5.866884622938899	0.0
42-43	0.0	0.0	0.0	7.11305216324154	0.0
44-45	0.0	0.0	0.0	8.28452069354113	0.0
46-47	0.0	0.0	0.0	9.648082342338075	0.0
48-49	0.0	0.0	0.0	10.870569077604785	0.0
50-51	0.0	0.0	0.0	11.996138635790611	0.0
52-53	0.0	0.0	0.0	13.034193689483356	0.0
54-55	0.0	0.0	0.0	14.225024760371149	0.0
56-57	0.0	0.0	0.0	15.66373814044389	0.0
58-59	0.0	0.0	0.0	16.798675546504224	0.0
60-61	0.0	0.0	0.0	17.818900346993445	0.0
62-63	0.0	0.0	0.0	18.74837194465378	0.0
64-65	0.0	0.0	0.0	19.74272198316991	0.0
66-67	0.0	0.0	0.0	20.857356581383264	0.0
68-69	0.0	0.0	0.0	22.143363593687532	0.0
70-71	0.0	0.0	0.0	23.58754445374651	0.0
72-73	0.0	6.9649426576271E-5	0.0	24.72955127660434	0.0
74-75	0.0	6.9649426576271E-5	0.0	25.86849352469281	0.0
76-77	0.0	6.9649426576271E-5	0.0	26.9501491194223	0.0
78-79	0.0	6.9649426576271E-5	0.0	28.318969299925755	0.0
80-81	0.0	6.9649426576271E-5	0.0	29.575793202494562	0.0
82-83	0.0	6.9649426576271E-5	0.0	30.811374029957612	0.0
84-85	0.0	6.9649426576271E-5	0.0	32.10194307970262	0.0
86-87	0.0	6.9649426576271E-5	0.0	33.28138646934519	0.0
88	0.0	6.9649426576271E-5	0.0	34.27719914581943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	1155	0.0	62.306133	1
ACCCATG	1315	0.0	54.700306	3
TACCCAT	1360	0.0	52.89037	2
ACTAGAC	50	3.004634E-5	47.013664	3
CGGATCC	50	0.0017003221	37.608303	94
GGATTAC	65	1.4153137E-4	36.14667	8
GGGCCGT	80	1.1570959E-5	35.257782	94
CGTATCA	530	0.0	34.59254	94
GTGCCGT	70	2.1824377E-4	33.578842	94
CTACATG	60	0.004146176	31.356682	1
GTCGGGA	75	3.270362E-4	31.34244	2
TAGACTC	60	0.0041589593	31.336966	5
GCGTCAG	95	3.7338817E-5	29.706331	1
GACTCAT	65	0.0061606346	28.917337	7
CGTGTCA	70	0.008827225	26.863071	94
CGCCGTT	90	9.506565E-4	26.116877	94
GCCGTAT	945	0.0	25.370678	94
CGGATCA	115	1.3753765E-4	24.527153	94
GCTCCGC	135	1.4600082E-5	24.365538	9
CCGGATC	215	8.0035534E-11	24.051819	94
>>END_MODULE