##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523064_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1435762 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969018542070344 33.0 31.0 34.0 30.0 34.0 2 32.011476832511235 33.0 31.0 34.0 30.0 34.0 3 32.165799066976284 33.0 31.0 34.0 30.0 35.0 4 35.26371083786867 37.0 35.0 37.0 32.0 37.0 5 35.22027466947865 36.0 35.0 37.0 32.0 37.0 6 35.46894541017244 36.0 35.0 37.0 33.0 37.0 7 35.2944875264842 36.0 35.0 37.0 32.0 37.0 8 35.35843127203534 36.0 35.0 37.0 33.0 37.0 9 36.46322788874479 37.0 35.0 39.0 33.0 39.0 10-11 36.28899706218719 37.0 35.0 39.0 32.0 39.0 12-13 36.346607237132616 37.0 35.0 39.0 32.0 39.0 14-15 37.168985528242146 38.0 36.5 40.0 32.0 41.0 16-17 36.9876466294553 37.5 36.0 40.0 31.5 41.0 18-19 37.07357452001098 38.0 36.0 40.0 32.0 41.0 20-21 37.19671888516342 38.0 36.0 40.0 32.5 41.0 22-23 37.10567628896712 38.0 35.5 40.0 32.0 41.0 24-25 36.96202225717076 38.0 36.0 40.0 31.5 41.0 26-27 36.49507578554106 37.0 35.5 40.0 31.0 41.0 28-29 36.50088176174046 37.5 35.5 40.0 31.0 41.0 30-31 36.1920311305077 36.5 35.0 40.0 30.5 41.0 32-33 35.940375563637986 37.0 35.0 40.0 30.0 41.0 34-35 35.68238503317402 37.0 35.0 40.0 30.0 41.0 36-37 35.423326777000646 36.5 35.0 40.0 30.0 41.0 38-39 35.21425521778679 36.5 34.5 40.0 27.5 41.0 40-41 35.34583830746321 37.0 35.0 40.0 28.0 41.0 42-43 35.24582765110095 37.5 35.0 40.0 27.5 41.0 44-45 34.867631961286065 37.0 35.0 40.0 25.5 41.0 46-47 34.46286188100813 37.0 34.0 40.0 23.5 41.0 48-49 34.0666990768665 37.0 33.5 40.0 21.5 41.0 50-51 33.720327951289974 36.5 33.0 40.0 18.5 41.0 52-53 33.77254865360693 37.0 33.0 40.0 16.5 41.0 54-55 33.63008005505091 37.5 33.0 40.0 14.5 41.0 56-57 33.233894963092766 37.0 33.0 40.0 12.5 41.0 58-59 33.027069597886 36.5 32.5 39.5 13.0 41.0 60-61 32.60112017172763 36.5 32.0 39.0 11.5 41.0 62-63 32.31422269150458 36.0 31.0 39.0 12.0 40.5 64-65 32.104106390892085 36.5 31.0 38.5 10.0 40.0 66-67 31.392048612513776 35.5 30.5 37.0 10.0 40.0 68-69 31.07650118891571 35.5 29.5 37.0 10.0 39.0 70-71 30.432603035879204 35.0 29.0 36.5 9.0 39.0 72-73 30.063747682415332 35.0 29.0 36.0 9.0 38.0 74-75 29.842963179134145 35.0 28.0 36.5 8.0 38.0 76-77 28.3052692577182 32.5 25.5 35.5 8.0 36.0 78-79 29.20419052739939 34.0 26.0 36.0 8.0 37.0 80-81 29.27088751478309 34.0 27.0 36.0 8.0 37.5 82-83 29.192160678441134 34.0 26.5 35.5 7.5 37.0 84-85 28.876817675910075 34.0 26.0 35.0 7.0 36.0 86-87 28.811485817287267 34.0 26.0 35.0 7.0 36.0 88-89 28.6785731200575 34.0 25.0 35.0 7.0 37.0 90-91 28.383374821175096 34.0 25.0 35.0 7.0 36.0 92-93 28.154458399094 33.0 24.0 35.0 7.0 36.5 94-95 28.285112017172764 34.0 24.5 35.0 7.0 36.5 96-97 28.015574656523853 33.5 24.0 35.0 7.0 36.5 98-99 27.86061060259291 33.0 24.0 35.0 7.0 36.0 100 26.65482440683066 32.0 19.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.16615882125080006 1101 2 -0.24513638829373363 1101 3 -0.20934691366922564 1101 4 -0.16527170665894886 1101 5 -0.25639094941697493 1101 6 -0.041677737743484045 1101 7 -0.13406403790613552 1101 8 -0.13533007026270383 1101 9 -0.25852294726715996 1101 10-11 -0.1658261244263386 1101 12-13 -0.22130609676487012 1101 14-15 -0.5433411403303978 1101 16-17 -0.48136753471641924 1101 18-19 -0.509611496001682 1101 20-21 -0.3757172319219251 1101 22-23 -0.9619362487905292 1101 24-25 -0.3407587968780703 1101 26-27 -0.3220280813588374 1101 28-29 -0.5662654049635449 1101 30-31 -0.2686043032005401 1101 32-33 -0.16356373574401317 1101 34-35 -0.23893355971369346 1101 36-37 -0.2636786659117263 1101 38-39 -0.3575595341124327 1101 40-41 -0.3788298031541686 1101 42-43 -0.38494196634357536 1101 44-45 -0.506867010872547 1101 46-47 -0.20702146543486322 1101 48-49 -0.30802351570891773 1101 50-51 -0.2559146260381411 1101 52-53 -0.1611697478339451 1101 54-55 -0.29218438588604556 1101 56-57 -0.35737586955585954 1101 58-59 -0.21697100072104547 1101 60-61 -0.23990761550957274 1101 62-63 -0.10644454217118238 1101 64-65 -0.2594820455875393 1101 66-67 -0.2572525138284938 1101 68-69 -0.22355648121171257 1101 70-71 -0.2634705242762152 1101 72-73 -0.25609092523709975 1101 74-75 -0.1727539172436714 1101 76-77 -0.22151727774203422 1101 78-79 -0.2378522793185347 1101 80-81 -0.3241021741840129 1101 82-83 -0.38484140351946294 1101 84-85 -0.264780136217837 1101 86-87 -0.2600822886607794 1101 88-89 -0.4382990256394166 1101 90-91 -0.4500753908590944 1101 92-93 -0.14745159587501533 1101 94-95 -0.4391676493723402 1101 96-97 -0.5705403825799102 1101 98-99 -0.608058680583099 1101 100 -0.6150198750194278 1102 1 -0.023761614113485763 1102 2 -0.0526372856687658 1102 3 0.006789047332595999 1102 4 -0.027378164285472906 1102 5 -0.0985525232390927 1102 6 -0.1588112324113382 1102 7 -0.03736811488354874 1102 8 -0.19218543656540277 1102 9 -0.24637538407458948 1102 10-11 -0.23929161311941272 1102 12-13 -0.1451004299862788 1102 14-15 -0.28411844079472104 1102 16-17 -0.27780929797150833 1102 18-19 -0.19916084004947976 1102 20-21 -0.15398194018820277 1102 22-23 -0.09506559816468751 1102 24-25 -0.06535845182289535 1102 26-27 -0.08796633037751178 1102 28-29 -0.1475698987861378 1102 30-31 -0.1926904280456725 1102 32-33 -0.05648547306207519 1102 34-35 -0.21100493287118383 1102 36-37 -0.354551203919911 1102 38-39 -0.3089718866244979 1102 40-41 -0.5390749312761614 1102 42-43 -0.44291905143789023 1102 44-45 -0.3256435702787783 1102 46-47 -0.4109427048545271 1102 48-49 -0.3589530225579125 1102 50-51 -0.3663719399341332 1102 52-53 -0.5661504687445174 1102 54-55 -0.37814538770395245 1102 56-57 -0.3813065458871634 1102 58-59 -0.4401313819028019 1102 60-61 -0.5846961823727028 1102 62-63 -0.28619947194434303 1102 64-65 -0.4606448699451917 1102 66-67 -0.4551677089930912 1102 68-69 -0.4566302512289617 1102 70-71 -0.4545940378178912 1102 72-73 -0.47618836279716703 1102 74-75 -0.38103089928459255 1102 76-77 -0.33404926481983566 1102 78-79 -0.34708539998363364 1102 80-81 -0.31060102207025864 1102 82-83 -0.4924965915050592 1102 84-85 -0.7006195581925247 1102 86-87 -0.7667489167306698 1102 88-89 -0.6084329371524504 1102 90-91 -0.5875662723708537 1102 92-93 -0.8748756663681654 1102 94-95 -0.887777586498192 1102 96-97 -0.9049159253470656 1102 98-99 -1.0786395427578732 1102 100 -1.039195737367887 1103 1 0.11150390570701063 1103 2 -0.02356127455009016 1103 3 -0.1446298119982785 1103 4 0.010558182402924388 1103 5 -0.09003713458773888 1103 6 -0.05937944594352729 1103 7 -0.09287610224665599 1103 8 -0.16634679134721608 1103 9 -0.17257795387331498 1103 10-11 -0.18567301947580717 1103 12-13 0.005710027821912433 1103 14-15 -0.05509992082910031 1103 16-17 -0.09070852658536666 1103 18-19 -0.24166912882252234 1103 20-21 -0.09066269125708715 1103 22-23 -0.056497965125693383 1103 24-25 -0.015817822880840993 1103 26-27 -0.08665547627108339 1103 28-29 -0.14599475282558672 1103 30-31 -0.29026854345218567 1103 32-33 -0.24444023924611002 1103 34-35 -0.4252906471568991 1103 36-37 -0.27062165569989816 1103 38-39 -0.0675679303400969 1103 40-41 -0.2429089772020987 1103 42-43 -0.40667757743170085 1103 44-45 -0.2253354623516941 1103 46-47 -0.16511779053308828 1103 48-49 -0.09899226166528763 1103 50-51 -0.10310125149153038 1103 52-53 0.011658504340694265 1103 54-55 -0.6798170964749772 1103 56-57 -0.04625675743228186 1103 58-59 -0.13929748110015794 1103 60-61 -0.06820040769336799 1103 62-63 -0.10441830671589258 1103 64-65 0.02990899870705732 1103 66-67 0.0854461625616274 1103 68-69 0.05748968759598583 1103 70-71 -0.03317830421961787 1103 72-73 -0.04817984517584506 1103 74-75 -0.1818154023489278 1103 76-77 0.011436842415118775 1103 78-79 -0.06540554198119963 1103 80-81 -0.9741103267063913 1103 82-83 0.12234645206542183 1103 84-85 0.2630962680613962 1103 86-87 0.048227249558305374 1103 88-89 0.0027379287871127644 1103 90-91 -0.14756777942049482 1103 92-93 -0.15963744089121334 1103 94-95 -0.09261465768675237 1103 96-97 -0.4058394118785067 1103 98-99 -0.3570689179462576 1103 100 -0.12464768481092392 1104 1 -0.09622592666044127 1104 2 -0.2332354219595132 1104 3 -0.09489468277718771 1104 4 -0.0933716379389935 1104 5 -0.12022403046629648 1104 6 -0.30620349723298546 1104 7 -0.3804504118883827 1104 8 -0.10755823448415214 1104 9 -0.25163346631552486 1104 10-11 -0.25426969042634084 1104 12-13 -0.16508484300756976 1104 14-15 -0.33100553051516357 1104 16-17 -0.3901600162060319 1104 18-19 -0.2987106118516323 1104 20-21 -0.3899146919066965 1104 22-23 -0.2913880804190754 1104 24-25 -0.21997343102156464 1104 26-27 -0.4236103305120764 1104 28-29 -0.40991557087647834 1104 30-31 -0.35751824239242325 1104 32-33 -0.24122369031540103 1104 34-35 -0.4262566233294862 1104 36-37 -0.3848732039871834 1104 38-39 -0.2644405052144947 1104 40-41 -0.20216911475396415 1104 42-43 -0.14099226538318277 1104 44-45 -0.23711177396358352 1104 46-47 -0.10139820346964257 1104 48-49 -0.16571289337661455 1104 50-51 0.08102004454617173 1104 52-53 0.03684862294952751 1104 54-55 0.007650837780204256 1104 56-57 -0.2157282577635442 1104 58-59 -0.1992262157725193 1104 60-61 -0.21793092900019673 1104 62-63 -0.08249980723258687 1104 64-65 -0.4665715662906642 1104 66-67 -0.5145950276963447 1104 68-69 -0.10491344084063314 1104 70-71 -0.30410680456268224 1104 72-73 -0.08795232659949193 1104 74-75 -0.29458960836877424 1104 76-77 -0.2978022504551454 1104 78-79 -0.3723912523895194 1104 80-81 -0.3623183931412761 1104 82-83 -0.17984787042226813 1104 84-85 -0.9500829848608383 1104 86-87 -0.46331585663670083 1104 88-89 -0.5044462029048269 1104 90-91 -0.5396068770110638 1104 92-93 -0.16351539410065286 1104 94-95 -0.3784624043323852 1104 96-97 -0.5560835470432437 1104 98-99 -0.2617952331264668 1104 100 -1.5040700883776736 1105 1 -0.09931227490643835 1105 2 -0.14814433193523158 1105 3 -0.31777425948135374 1105 4 -0.06503349994199681 1105 5 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-0.6014063708109063 1105 66-67 -0.6930816634498846 1105 68-69 -0.23289649709215965 1105 70-71 -0.10805032466559084 1105 72-73 -0.4470504619928164 1105 74-75 -0.588379070323672 1105 76-77 -0.6959260044696016 1105 78-79 -0.3141268447422192 1105 80-81 -0.3757104967234248 1105 82-83 -0.4344920108839361 1105 84-85 -0.3849654498794628 1105 86-87 -0.31623715834199473 1105 88-89 -0.4876497386077254 1105 90-91 -0.3935172443830375 1105 92-93 -0.3291138367497375 1105 94-95 -0.4393983373693864 1105 96-97 -0.38541924574945696 1105 98-99 -0.42476738372922895 1105 100 -0.14553847302878964 1106 1 -0.09098303234792837 1106 2 -0.19246708025588788 1106 3 -0.1203757797620355 1106 4 -0.060006628332821776 1106 5 -0.21099177416247983 1106 6 -0.10900238950575414 1106 7 -0.09670349298580305 1106 8 -0.1492388093541379 1106 9 -0.21188934826233208 1106 10-11 -0.2690548807177464 1106 12-13 -0.28863888820171013 1106 14-15 -0.08930560194986725 1106 16-17 -0.22690643169507752 1106 18-19 -0.3651013977530084 1106 20-21 -0.3795316518325791 1106 22-23 -0.2730026135220456 1106 24-25 -0.13902089215380187 1106 26-27 0.018012998736686825 1106 28-29 -0.07375194081909342 1106 30-31 -0.11456657552823657 1106 32-33 0.02791200409627237 1106 34-35 -0.03517280551951529 1106 36-37 -0.09405430347278809 1106 38-39 -0.07894240307265221 1106 40-41 -0.11153102994345687 1106 42-43 -0.17654272035812113 1106 44-45 0.020906962153134145 1106 46-47 -0.2339171956422632 1106 48-49 -0.18489044830904078 1106 50-51 -0.16297161161912044 1106 52-53 -0.38845752967718994 1106 54-55 -0.36570613678055963 1106 56-57 -0.2296871105025744 1106 58-59 -0.300846979025728 1106 60-61 -0.25095371525820553 1106 62-63 -0.22909371995177708 1106 64-65 -0.14638098675621336 1106 66-67 -0.12753416902289771 1106 68-69 -0.05489147765831959 1106 70-71 -0.05738687536675968 1106 72-73 -0.03151646181005674 1106 74-75 -0.08212762261439721 1106 76-77 -0.018788016790804107 1106 78-79 0.07405527867805262 1106 80-81 0.04617695490449236 1106 82-83 0.0634859775598926 1106 84-85 0.34110776755212413 1106 86-87 0.27143930280839257 1106 88-89 0.1054626716342959 1106 90-91 0.18168713497735567 1106 92-93 0.11403236222979984 1106 94-95 0.03200555279808981 1106 96-97 0.19189002320251447 1106 98-99 -0.11182257837573317 1106 100 0.08995733950534657 1107 1 -0.01720940668531412 1107 2 -0.16992122337337534 1107 3 -0.1943578112267481 1107 4 -0.10509783502777026 1107 5 -0.12319664670027919 1107 6 -0.15409410327077921 1107 7 -0.2572621625150475 1107 8 -0.26915936161429954 1107 9 -0.1372988327039053 1107 10-11 -0.08298729030460805 1107 12-13 -0.08334421456722652 1107 14-15 -0.2559019548990378 1107 16-17 -0.17546746822744552 1107 18-19 -0.2782260954124709 1107 20-21 -0.13161139975790093 1107 22-23 0.09177193805052042 1107 24-25 -0.08486707548802741 1107 26-27 -0.18455059545716068 1107 28-29 -0.08315023118161946 1107 30-31 -0.023526960171309952 1107 32-33 0.032211197753433396 1107 34-35 -0.25094887120334874 1107 36-37 -0.36345125517031107 1107 38-39 -0.3194523330259855 1107 40-41 -0.38297299526595197 1107 42-43 -0.27206458167039926 1107 44-45 -0.23051131285530602 1107 46-47 0.010662478757893723 1107 48-49 -0.08527298197595456 1107 50-51 -0.020397450084850277 1107 52-53 -0.2966251102531672 1107 54-55 -0.17266734693373564 1107 56-57 -0.09110713997349507 1107 58-59 -0.22515789614309512 1107 60-61 -0.14607717161011635 1107 62-63 -0.39986528792401543 1107 64-65 -0.06838498855327657 1107 66-67 -0.23142674318443213 1107 68-69 -0.06277768504795489 1107 70-71 0.06323901137203691 1107 72-73 -0.21176947963763126 1107 74-75 -0.10505504973068369 1107 76-77 -0.13189988557729393 1107 78-79 -0.08820182207778515 1107 80-81 0.09917211644180313 1107 82-83 0.04863212022710073 1107 84-85 0.044892893564107084 1107 86-87 0.016312824318813313 1107 88-89 -0.113988637975595 1107 90-91 0.10475218091283267 1107 92-93 0.3897131798701601 1107 94-95 0.2016742433504639 1107 96-97 0.04659517716420325 1107 98-99 0.04857991356946201 1107 100 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-0.20559304937973621 2316 58-59 -0.3329776898860999 2316 60-61 -1.8570575561812 2316 62-63 -0.4606077005628606 2316 64-65 -0.45962284706087075 2316 66-67 -0.6594524334261358 2316 68-69 -0.3161248110521804 2316 70-71 -0.39676055779017716 2316 72-73 -0.22395845212468046 2316 74-75 -0.3081919884435642 2316 76-77 -0.5949005916237056 2316 78-79 -0.7681260561756957 2316 80-81 -0.4718446783185044 2316 82-83 -0.6563334611093588 2316 84-85 -1.1597302156954719 2316 86-87 -0.6199015974924826 2316 88-89 -0.5698306225023622 2316 90-91 -0.34971771715446565 2316 92-93 -0.104480621517137 2316 94-95 -0.10761035490209636 2316 96-97 -0.2367615591604313 2316 98-99 -0.15966025475622558 2316 100 -0.4740952673216299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 29.0 13 184.0 14 822.0 15 2233.0 16 5013.0 17 9692.0 18 14238.0 19 19410.0 20 24991.0 21 29958.0 22 30563.0 23 28169.0 24 26748.0 25 26049.0 26 26974.0 27 29502.0 28 32634.0 29 36094.0 30 41736.0 31 48402.0 32 58173.0 33 73498.0 34 99134.0 35 170807.0 36 253906.0 37 197429.0 38 139527.0 39 9838.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.18889958847622 18.24271123517058 13.780820734220859 17.787568442132343 2 14.39244758888314 25.58279657374753 34.08072186182844 25.944033975540897 3 27.34554891409579 28.580224299013345 30.88520242212846 13.189024364762405 4 13.251160882626008 19.67980743313128 34.198379813910535 32.87065187033218 5 9.706872198340108 42.21949969214825 33.454500625454465 14.619127484057179 6 25.922750427995727 33.22890562641998 16.289468588805107 24.55887535677919 7 19.24594744811466 38.21831194863773 21.523622996011873 21.01211760723574 8 26.29952081127765 26.79577366690812 21.43624282609907 25.46846269571516 9 24.162918366693088 19.799312142263133 22.902194096236006 33.13557539480777 10-11 24.598923777060545 26.1996068986364 23.36212408463241 25.839345239670642 12-13 31.71596466814045 24.906321260268097 23.973921789048013 19.403792282543435 14-15 27.543771049634476 25.11975870632038 28.710534656361997 18.625935587683156 16-17 19.181210260067797 29.83048474620422 30.698329294768346 20.28997569895963 18-19 19.754074839701843 30.2669244624109 31.441666515759575 18.53733418212768 20-21 22.169121884950147 25.259698492392314 28.727986702434233 23.84319292022331 22-23 19.138819132446155 27.917718237060157 28.511833018224976 24.43162961226871 24-25 24.168908311056196 28.524303790062284 26.054571213246202 21.252216685635318 26-27 23.175607051254367 23.799899132455526 32.14759401649268 20.876899799797428 28-29 20.152021596763134 28.607212875861453 31.37932223472583 19.861443292649582 30-31 21.888471807288333 27.202315069895803 30.042517711649953 20.866695411165914 32-33 19.06290475425942 28.27689222561862 32.641751711134276 20.018451308987682 34-35 19.914435641971252 26.819069762744192 31.504282571346877 21.76221202393768 36-37 20.22748564284197 26.846300920460127 32.61688589846729 20.30932753823061 38-39 21.721918790504883 25.24405231849267 31.842302030394094 21.191726860608348 40-41 18.40302627899258 28.072659114203237 31.400152052270318 22.124162554533864 42-43 20.070705441936873 27.54419612523591 29.29438392894768 23.090714503879536 44-45 21.4328874896172 24.94334516871072 29.873630210337698 23.750137131334377 46-47 21.344767401967697 28.01099520770835 31.039135597228768 19.605101793095184 48-49 20.689133901272257 26.413269958613235 31.54934610859202 21.348250031522493 50-51 16.104983190596208 27.95780721223039 33.68073696661665 22.25647263055675 52-53 17.542630259125104 29.62196990805238 28.08773335190861 24.747666480913903 54-55 16.315837679879447 26.903219537813293 28.32076761565725 28.46017516665001 56-57 20.968562027587627 24.819532553791976 26.651405988370957 27.560499430249436 58-59 22.362579111829962 24.37772790406415 28.649000505702737 24.61069247840315 60-61 20.29855892124564 27.057141663126078 29.50537357302557 23.138925842602717 62-63 16.54657944701141 28.16964092934623 29.836316882603104 25.44746274103925 64-65 15.444781857692094 31.531453724856522 27.9777260823536 25.046038335097787 66-67 19.336430899229743 27.751956989756582 24.99145046738606 27.920161643627623 68-69 19.35523413881762 26.981082860906913 26.163154535067278 27.500528465208184 70-71 21.358897030144313 27.35241265949741 24.792966791107148 26.49572351925113 72-73 21.269417944015583 24.666352306611103 28.87848683692723 25.18574291244608 74-75 20.384020517128313 28.571856442789944 25.486346699487385 25.557776340594362 76-77 19.891417647818862 26.447593464998754 27.075482113235882 26.585506773946506 78-79 20.593664045201297 26.710668094066286 28.048576628583326 24.647091232149087 80-81 20.93000822211767 26.10851454088997 26.676192389892055 26.285284847100304 82-83 18.978688875694104 25.869047764151553 27.962723380248338 27.18953997990601 84-85 19.959714701522085 26.800762941147177 26.088979386868065 27.15054297046267 86-87 20.433122678275854 26.467071957385617 25.693822677126636 27.40598268721189 88-89 20.309773329028992 25.26492076145065 25.310227792328728 29.115078117191633 90-91 21.679056164718453 23.881619408271167 25.984235515849107 28.455088911161276 92-93 21.09845924430779 25.81050477570535 26.904910407858157 26.1861255721287 94-95 19.958015311038775 25.11294529070462 27.311430206200683 27.617609192055927 96-97 18.971887270479456 25.616486300594644 27.992651860106736 27.41897456881916 98-99 21.307497958394787 25.587085562847488 25.03748021514775 28.067936263609976 100 19.80468894196179 23.691268950751553 26.42292136365314 30.081120743633516 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2739.0 1 1756.5 2 901.0 3 988.0 4 828.0 5 786.5 6 1143.5 7 1910.5 8 2741.5 9 3136.5 10 2624.0 11 1993.0 12 1895.5 13 1926.5 14 2000.5 15 2104.5 16 2218.5 17 2338.5 18 2523.0 19 2816.5 20 3083.5 21 3382.0 22 3807.5 23 4237.0 24 4892.5 25 5690.5 26 6627.0 27 7782.5 28 9402.0 29 11373.5 30 13157.0 31 15275.0 32 17370.5 33 18583.5 34 19411.5 35 19674.0 36 19918.0 37 19746.0 38 18934.5 39 18194.0 40 17731.0 41 17395.5 42 16784.0 43 16476.5 44 17229.5 45 20287.0 46 32853.0 47 80113.0 48 162130.5 49 195521.5 50 209895.0 51 187452.0 52 92791.0 53 32101.5 54 14376.0 55 9973.0 56 7688.5 57 6008.0 58 4548.5 59 3367.0 60 2703.5 61 2161.5 62 1625.5 63 1243.0 64 1108.0 65 918.0 66 747.5 67 601.5 68 415.0 69 249.0 70 151.0 71 119.5 72 109.0 73 107.0 74 87.5 75 55.5 76 32.5 77 17.5 78 12.0 79 9.5 80 9.5 81 6.5 82 1.5 83 1.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04234685135837277 2 0.00982056914725421 3 0.0 4 6.9649426576271E-5 5 4.1789655945762596E-4 6 0.0 7 0.0 8 1.39298853152542E-4 9 0.0 10-11 0.0 12-13 2.78597706305084E-4 14-15 0.011631454238237256 16-17 0.001462637958101691 18-19 0.0 20-21 7.313189790508455E-4 22-23 8.706178322033874E-4 24-25 0.00417896559457626 26-27 0.015044276140474535 28-29 0.01229312379071183 30-31 0.008706178322033875 32-33 0.006372922531728797 34-35 0.008183807622711842 36-37 0.004666511580610157 38-39 0.00386554317498304 40-41 0.0049799340002033765 42-43 0.006094324825423712 44-45 0.006581870811457609 46-47 0.01584524454610165 48-49 0.019432190014779607 50-51 0.017830253203525374 52-53 0.011283207105355901 54-55 0.0030297500560677883 56-57 0.003203873622508466 58-59 0.009472322014372855 60-61 0.002228781650440672 62-63 0.0 64-65 1.39298853152542E-4 66-67 7.661436923389809E-4 68-69 3.48247132881355E-5 70-71 1.39298853152542E-4 72-73 0.002855626489627111 74-75 0.005815727119118628 76-77 0.005397830559661003 78-79 0.0035521207553898208 80-81 3.48247132881355E-5 82-83 6.616695524745745E-4 84-85 1.741235664406775E-4 86-87 1.741235664406775E-4 88-89 1.741235664406775E-4 90-91 2.0894827972881298E-4 92-93 3.8307184616949045E-4 94-95 3.48247132881355E-5 96-97 0.0017064109511186396 98-99 3.134224195932195E-4 100 6.9649426576271E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1435762.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.375479769879302 #Duplication Level Percentage of deduplicated Percentage of total 1 73.61328726670774 13.526794709637018 2 10.003381393106343 3.6763386483882528 3 4.256817673937375 2.3466320115450268 4 2.4608253716476165 1.8087538733566597 5 1.6622785126721888 1.527258259075643 6 1.2154669890922907 1.3400873441412897 7 0.8571809086825581 1.1025777312636174 8 0.7047015394882921 1.0359383106135929 9 0.5793940646746697 0.9581979522795779 >10 4.042322006979794 14.459269675960268 >50 0.3412723019933129 4.278050621729387 >100 0.20233385361115389 7.144105197121816 >500 0.022776794027522 2.9404142553338914 >1k 0.024295246962690135 11.587616417652363 >5k 0.005314585273088467 7.241065693762957 >10k+ 0.008351491143424735 25.026899298138645 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 46305 3.2251166976142285 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 30158 2.1004874066871806 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 28814 2.0068785773686724 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 27250 1.8979468742033847 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20741 1.4445987566184366 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 15325 1.067377462281353 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13924 0.9697986156479974 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13033 0.9077409765685398 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 12839 0.8942289878127433 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 12810 0.8922091544420314 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12770 0.8894231773789807 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12573 0.8757022403434553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12127 0.8446385960904385 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11997 0.8355841706355233 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11836 0.8243706129567435 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11717 0.8160823311941673 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11050 0.7696261636677945 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11010 0.7668401866047437 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10945 0.762312973877286 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10741 0.7481044908557267 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10717 0.7464329046178964 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10097 0.7032502601406082 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9584 0.6675201043069812 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9367 0.6524061787399305 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 9044 0.6299094139557949 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 8541 0.5948757523879306 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7970 0.5551059298128799 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7909 0.5508573147917273 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7735 0.5387383145674561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7029 0.4895658194046088 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6901 0.48065069280284617 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 6399 0.4456866806615581 TruSeq Adapter, Index 3 (95% over 21bp) GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6326 0.44060227252149037 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5770 0.40187719134508365 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5724 0.39867331772257514 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 5507 0.3835593921555244 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4960 0.34546115581830417 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4860 0.3384962131606771 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4808 0.33487444297871094 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4653 0.32407878185938893 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 4652 0.3240091324328127 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 4616 0.32150175307606693 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4592 0.31983016683823645 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 4573 0.3185068277332873 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4395 0.30610922980271105 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 4320 0.3008855228094907 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 4236 0.2950349709770839 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 4226 0.29433847671132124 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4203 0.292736539900067 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4168 0.29029880996989754 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4113 0.2864680915082026 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 3946 0.2748366372699654 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3792 0.26411062557721965 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 3638 0.25338461388447386 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3478 0.24224070563227054 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3209 0.22350500988325364 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3176 0.22120657880623668 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 3109 0.21654006722562655 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 3053 0.21263969933735535 RNA PCR Primer, Index 3 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3051 0.2125004004842028 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3038 0.21159495793871128 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 2927 0.2038638715887452 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2851 0.1985705151689486 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2640 0.18387448616135543 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 2601 0.18115815852488087 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2532 0.17635234809111816 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2499 0.17405391701410122 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2444 0.1702231985524063 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2270 0.15810419832813516 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2258 0.1572684052092199 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2063 0.14368676702684707 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2024 0.14097043939037252 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1930 0.13442339329220301 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 1909 0.13296075533410134 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1839 0.12808529547376238 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 1762 0.1227222896273895 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1735 0.12084175510983018 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1731 0.12056315740352509 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1687 0.11749858263416918 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1602 0.11157838137518614 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1562 0.1087924043121353 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 1509 0.10510098470359293 TruSeq Adapter, Index 3 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.741235664406775E-4 0.0 12-13 0.0 0.0 0.0 3.830718461694905E-4 0.0 14-15 0.0 0.0 0.0 8.35793118915252E-4 0.0 16-17 0.0 0.0 0.0 9.75091972067794E-4 0.0 18-19 0.0 0.0 0.0 0.0017412356644067749 0.0 20-21 6.9649426576271E-5 0.0 0.0 0.00773108634996608 0.0 22-23 6.9649426576271E-5 0.0 0.0 0.02483002057444061 0.0 24-25 6.9649426576271E-5 0.0 0.0 0.0471874865054236 0.0 26-27 6.9649426576271E-5 0.0 0.0 0.09106662524847434 0.0 28-29 6.9649426576271E-5 0.0 0.0 0.23266390947803328 0.0 30-31 6.9649426576271E-5 0.0 0.0 0.5182962078673207 0.0 32-33 6.9649426576271E-5 0.0 0.0 1.0543181947983022 0.0 34-35 6.9649426576271E-5 0.0 0.0 1.9688848151713167 0.0 36-37 6.9649426576271E-5 0.0 0.0 3.1498604921985676 0.0 38-39 6.9649426576271E-5 0.0 0.0 4.458816990559717 0.0 40-41 6.9649426576271E-5 0.0 0.0 5.866745324085747 0.0 42-43 6.9649426576271E-5 0.0 0.0 7.115002347185675 0.0 44-45 6.9649426576271E-5 0.0 0.0 8.289152380408453 0.0 46-47 6.9649426576271E-5 0.0 0.0 9.655674129834889 0.0 48-49 6.9649426576271E-5 0.0 0.0 10.87777779325543 0.0 50-51 6.9649426576271E-5 0.0 0.0 12.00251155832234 0.0 52-53 6.9649426576271E-5 0.0 0.0 13.03913879877027 0.0 54-55 6.9649426576271E-5 0.0 0.0 14.227183892595011 0.0 56-57 6.9649426576271E-5 0.0 0.0 15.66338989331101 0.0 58-59 6.9649426576271E-5 0.0 0.0 16.79916309249026 0.0 60-61 6.9649426576271E-5 0.0 0.0 17.817159111329033 0.0 62-63 6.9649426576271E-5 0.0 0.0 18.74544666873758 0.0 64-65 6.9649426576271E-5 0.0 0.0 19.73885643999493 0.0 66-67 6.9649426576271E-5 0.0 0.0 20.849938917452892 0.0 68-69 6.9649426576271E-5 0.0 0.0 22.13542355905784 0.0 70-71 6.9649426576271E-5 0.0 0.0 23.58155460306095 0.0 72-73 6.9649426576271E-5 0.0 0.0 24.726800124254577 0.0 74-75 6.9649426576271E-5 0.0 0.0 25.86685676316827 0.0 76-77 6.9649426576271E-5 0.0 0.0 26.950009820569147 0.0 78-79 6.9649426576271E-5 0.0 0.0 28.320292639030704 0.0 80-81 6.9649426576271E-5 0.0 0.0 29.582409898019307 0.0 82-83 6.9649426576271E-5 0.0 0.0 30.820950826111847 0.0 84-85 6.9649426576271E-5 0.0 0.0 32.11367900808072 0.0 86-87 6.9649426576271E-5 0.0 0.0 33.294933282814284 0.0 88 6.9649426576271E-5 0.0 0.0 34.2943329047572 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 905 0.0 67.52104 1 TACCGTA 25 0.005238416 56.394257 7 ACCCATG 1170 0.0 51.815235 3 TACCCAT 1235 0.0 49.08982 2 TATAACG 50 0.0017027291 37.59748 2 ACCTATT 70 2.1838976E-4 33.575027 1 GCTTATG 575 0.0 33.516632 1 ATTGCTC 30 0.004159121 31.337782 50-51 CTTAACG 45 2.5942956E-5 31.33505 20-21 CTTATGT 630 0.0 30.58525 2 TTATGTA 645 0.0 29.872927 3 CTATATG 95 3.7519618E-5 29.685322 9 GAGCGCT 95 3.7519618E-5 29.685322 9 AGACGTT 95 3.7555306E-5 29.681185 6 GCGTCAG 80 4.7784724E-4 29.378147 1 GTACTGG 115 4.1938492E-6 28.611761 1 TACTGGG 115 4.199539E-6 28.60678 2 TGTACCG 115 4.2006777E-6 28.605783 5 GTATAGC 100 5.320557E-5 28.203022 1 AGTACGC 185 3.2923708E-10 27.944073 2 >>END_MODULE