FastQCFastQC Report
Mon 6 Jun 2016
ERR523063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556189
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235700.4242116313897889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192850.34709042474977003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116790.2101980332202522No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100350.18060940691542351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG96250.054.811692
GTACCTG108300.049.2438431
ACCTGGG110950.047.210543
CCTGGGG99450.036.1655124
TATAACG5200.032.552192
GGTATCA173000.031.8874131
GTATCAA249350.029.2340951
GTACCCG11000.026.0798131
CTGGGGG61850.024.7032555
TATCACG6100.024.6661422
TCAACGC291850.024.5989914
TAACGCA7100.024.500864
CAACGCA295100.024.3584025
ATCAACG296400.024.1920533
TATCAAC300350.023.9991362
AACGCAG303400.023.8549486
GTACATG231100.022.9549561
CATGGGG133950.021.7965034
TACATGG237950.021.7364222
ACGCAGA333850.021.6226637
Produced by FastQC (version 0.11.5)