FastQCFastQC Report
Mon 6 Jun 2016
ERR523063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556189
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207800.37399735682137525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193880.34894421338079035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122820.22105079578826423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82820.1490590042923306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG92650.050.5238152
GTACCTG104150.045.597371
ACCTGGG108700.042.4553953
CCTGGGG93850.032.648584
GGTATCA187700.030.3860451
GTATCAA261450.026.796331
TATAACG6850.024.699842
GTACCCG9550.024.6175231
TAACGCA7450.024.601374
CTGGGGG58450.024.4421845
TATCACG4800.022.5200212
TCAACGC312650.022.1709614
CAACGCA315350.021.996045
ATCAACG315500.021.9259993
TATCAAC318000.021.9029852
ATAACGC7600.021.64243
AACGCAG324950.021.5052936
GTACATG236950.021.47081
TACATGG243700.020.693142
GAGTACT203200.020.11327612-13
Produced by FastQC (version 0.11.5)