##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523063_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5556189
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91942984660889	33.0	31.0	34.0	30.0	34.0
2	32.05477621441603	33.0	31.0	34.0	30.0	34.0
3	32.20983861420121	33.0	31.0	34.0	30.0	35.0
4	35.36214696800271	37.0	35.0	37.0	32.0	37.0
5	35.30513522848125	36.0	35.0	37.0	32.0	37.0
6	35.446624115918304	36.0	35.0	37.0	33.0	37.0
7	35.2921934441035	36.0	35.0	37.0	32.0	37.0
8	35.27642814166329	36.0	35.0	37.0	32.0	37.0
9	36.51244837063678	37.0	35.0	39.0	33.0	39.0
10-11	36.31183640441317	37.0	35.0	39.0	32.0	39.0
12-13	36.324614497455	37.0	35.0	39.0	32.0	39.0
14-15	37.408045424660685	38.0	37.0	40.0	32.0	41.0
16-17	37.22062280458782	38.0	36.5	40.0	32.0	41.0
18-19	37.104464588947565	38.0	36.0	40.0	31.5	41.0
20-21	37.13713086433885	38.0	36.0	40.0	32.5	41.0
22-23	36.96762943089229	38.0	35.5	40.0	32.0	41.0
24-25	36.876936601688676	38.0	36.0	40.0	31.0	41.0
26-27	36.55135696427893	38.0	35.5	40.0	31.0	41.0
28-29	36.459732111344664	38.0	35.5	40.0	31.0	41.0
30-31	36.20800984271773	37.0	35.0	40.0	30.5	41.0
32-33	36.13076058067859	37.5	35.0	40.0	30.0	41.0
34-35	35.89706253692954	37.0	35.0	40.0	30.0	41.0
36-37	35.666504235187105	37.0	35.0	40.0	29.5	41.0
38-39	35.61050632006939	36.5	34.5	40.0	28.5	41.0
40-41	35.79089777183606	37.5	35.0	40.0	29.5	41.0
42-43	35.80934557841715	37.5	35.0	40.0	29.5	41.0
44-45	35.6486689887619	37.0	35.0	40.0	29.0	41.0
46-47	35.50702828863453	37.0	34.5	40.0	27.5	41.0
48-49	35.27005848793121	37.0	34.0	40.0	27.0	41.0
50-51	35.0948487353472	37.0	34.0	40.0	26.5	41.0
52-53	35.072077821686776	37.0	34.0	40.0	26.5	41.0
54-55	34.87236422303129	37.5	34.0	40.0	26.0	41.0
56-57	34.63844183486199	37.0	34.0	39.0	25.5	41.0
58-59	34.4399252797196	36.0	33.5	39.0	25.5	41.0
60-61	34.23501162397463	36.5	33.0	39.0	24.5	41.0
62-63	33.98836081709963	36.0	33.0	39.0	24.5	40.0
64-65	33.77999803462409	36.5	32.5	38.0	24.0	40.0
66-67	33.30510094239055	35.5	32.0	37.5	23.0	40.0
68-69	33.15814625816364	35.5	32.0	37.0	23.0	40.0
70-71	32.72815890532162	35.0	31.5	37.0	23.0	39.0
72-73	32.49774917663888	35.0	31.0	36.5	22.5	39.0
74-75	32.25752138741141	35.0	31.0	37.0	22.0	39.0
76-77	30.78800505166401	33.5	29.0	36.0	20.5	36.5
78-79	31.920779782689177	34.5	31.0	36.5	23.0	37.0
80-81	32.03288504044769	35.0	31.0	36.0	21.5	37.5
82-83	31.938365937515805	35.0	31.0	37.0	22.5	37.0
84-85	31.602489584137615	34.5	31.0	35.5	22.0	36.5
86-87	31.461973035834454	34.5	31.0	36.0	21.0	36.0
88-89	31.37428388415153	34.0	30.5	35.5	20.5	37.0
90-91	31.133158177304622	34.0	30.5	35.0	20.0	36.5
92-93	30.898077441210155	34.0	30.0	35.0	20.0	36.5
94-95	30.977479077835547	34.0	30.0	35.0	20.0	36.5
96-97	30.708931877587318	34.0	29.5	35.0	19.5	36.5
98-99	30.54311462407056	34.0	29.5	35.0	19.0	36.0
100	29.285854746841764	33.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12575422683168824
1101	2	-0.14696405173862814
1101	3	-0.1415328436690899
1101	4	-0.16804751902343185
1101	5	-0.307231350517867
1101	6	-0.20141801722905228
1101	7	-0.12219622581494605
1101	8	-0.2643853659745261
1101	9	-0.26648132043467854
1101	10-11	-0.22580315505691573
1101	12-13	-0.17993774663524675
1101	14-15	-0.42687552666155426
1101	16-17	-0.33909310000939996
1101	18-19	-0.551035914388649
1101	20-21	-0.3829260419734126
1101	22-23	-0.9603471947637274
1101	24-25	-0.5456628293046961
1101	26-27	-0.5088085117260519
1101	28-29	-0.46627480880267314
1101	30-31	-0.5253430141919679
1101	32-33	-0.541570844142953
1101	34-35	-0.532942842170911
1101	36-37	-0.5565675128894227
1101	38-39	-0.56452660518935
1101	40-41	-0.5223167175430135
1101	42-43	-0.5184513721183066
1101	44-45	-0.6988071123611377
1101	46-47	-0.6373146223538129
1101	48-49	-0.6612657338525807
1101	50-51	-0.6348164055101932
1101	52-53	-0.6904355045417248
1101	54-55	-0.7537197260870769
1101	56-57	-0.8079274347204048
1101	58-59	-0.8182351881890639
1101	60-61	-1.030171554900278
1101	62-63	-0.8444892663988455
1101	64-65	-0.8213346066769205
1101	66-67	-0.8099155381572558
1101	68-69	-0.7563293320106155
1101	70-71	-0.9724989044498002
1101	72-73	-0.9226183431937578
1101	74-75	-0.8154842604146992
1101	76-77	-0.9125250389236719
1101	78-79	-0.720610958058888
1101	80-81	-0.6919944092183457
1101	82-83	-0.6720248230455574
1101	84-85	-0.7102007544550695
1101	86-87	-0.7783159121362502
1101	88-89	-0.9007850876397043
1101	90-91	-0.9222318002280723
1101	92-93	-0.7993588523696538
1101	94-95	-0.89399501176446
1101	96-97	-1.0659955895342783
1101	98-99	-0.971990765942806
1101	100	-0.8435467802554086
1102	1	-0.11011466272892889
1102	2	-0.12567023118906562
1102	3	-0.20182596235598993
1102	4	-0.15686761116055692
1102	5	-0.18537705476561683
1102	6	-0.1357140476985066
1102	7	-0.09387551013504947
1102	8	-0.15018154360873126
1102	9	-0.08242910366933387
1102	10-11	-0.21315768569788673
1102	12-13	-0.13582785349452564
1102	14-15	-0.32507032580881656
1102	16-17	-0.32305978946111225
1102	18-19	-0.2680377052775782
1102	20-21	-0.34673224270897407
1102	22-23	-0.24308968426897337
1102	24-25	-0.22843740619095598
1102	26-27	-0.32399843374753345
1102	28-29	-0.35534981144112976
1102	30-31	-0.37209216362086295
1102	32-33	-0.3284218811203772
1102	34-35	-0.3145059021785741
1102	36-37	-0.47028959514681645
1102	38-39	-0.4223239700779473
1102	40-41	-0.3614879468374781
1102	42-43	-0.28283004622456787
1102	44-45	-0.2011716315390757
1102	46-47	-0.26446933328313804
1102	48-49	-0.2981841536203973
1102	50-51	-0.1690616114418404
1102	52-53	-0.26898438059266994
1102	54-55	-0.10142473666540752
1102	56-57	-0.33902359175083774
1102	58-59	-0.3262402125860575
1102	60-61	-0.34631748001935847
1102	62-63	-0.587302874769982
1102	64-65	-0.49539424094323437
1102	66-67	-0.5370745891514872
1102	68-69	-0.402885942958612
1102	70-71	-0.4152433521298704
1102	72-73	-0.4290125576423591
1102	74-75	-0.48120738584910683
1102	76-77	-0.338534189185804
1102	78-79	-0.39859128915438546
1102	80-81	-0.42338220228717205
1102	82-83	-0.29496834024126173
1102	84-85	-0.5272965079268879
1102	86-87	-0.5106330752796104
1102	88-89	-0.47015007758871263
1102	90-91	-0.5572635692903418
1102	92-93	-0.49795789857740047
1102	94-95	-0.6290078413938112
1102	96-97	-0.5935291849370294
1102	98-99	-0.6656085496622168
1102	100	-0.5703147463320697
1103	1	-0.12506552436103036
1103	2	-0.1549938661563779
1103	3	-0.2636132596791576
1103	4	-0.18882185326171452
1103	5	-0.2071825398031919
1103	6	-0.24162528952658135
1103	7	-0.11077171324780011
1103	8	-0.1468620821400961
1103	9	-0.2557866831477611
1103	10-11	-0.2433822350267647
1103	12-13	-0.20129420508415308
1103	14-15	-0.180853927894141
1103	16-17	-0.2713983541030345
1103	18-19	-0.35878317865608267
1103	20-21	-0.32013745503434876
1103	22-23	-0.31035831316692253
1103	24-25	-0.2939015838676866
1103	26-27	-0.34939491232611175
1103	28-29	-0.2817396370871137
1103	30-31	-0.5082659982534565
1103	32-33	-0.38555255350611617
1103	34-35	-0.46255200997386936
1103	36-37	-0.4963081751317304
1103	38-39	-0.4370422230304527
1103	40-41	-0.5122590026848926
1103	42-43	-0.4582364695919665
1103	44-45	-0.4421558281585263
1103	46-47	-0.588047744354327
1103	48-49	-0.5391799444419476
1103	50-51	-0.587595537008859
1103	52-53	-0.5411277538056822
1103	54-55	-1.2715224302404522
1103	56-57	-0.7225547592089967
1103	58-59	-0.5234002403872893
1103	60-61	-0.6324347998180286
1103	62-63	-0.6168085318089354
1103	64-65	-0.5829726683015153
1103	66-67	-0.5012725384822048
1103	68-69	-0.5811117415627649
1103	70-71	-0.6852014942997755
1103	72-73	-0.7730023372151891
1103	74-75	-0.7772433212503529
1103	76-77	-0.5959390540992011
1103	78-79	-0.6123251080823451
1103	80-81	-1.3481838810448181
1103	82-83	-0.3781085173882488
1103	84-85	-0.25354790708138
1103	86-87	-0.39752771731586733
1103	88-89	-0.43860629094577064
1103	90-91	-0.41832134285433753
1103	92-93	-0.38819519048648843
1103	94-95	-0.45458385412148417
1103	96-97	-0.5972672281573175
1103	98-99	-0.4374501458084161
1103	100	-0.5184880418342601
1104	1	-0.13691341565498405
1104	2	-0.28064790554052266
1104	3	-0.17972422339592953
1104	4	-0.062141757750183046
1104	5	-0.0932261959413978
1104	6	-0.21763181918974084
1104	7	-0.321365584760116
1104	8	-0.09889937422254036
1104	9	-0.18723549440771592
1104	10-11	-0.1767275551813512
1104	12-13	-0.26182882970456234
1104	14-15	-0.38959801545652084
1104	16-17	-0.35000377081687617
1104	18-19	-0.34714165445966216
1104	20-21	-0.4218045131879009
1104	22-23	-0.2753581695278555
1104	24-25	-0.23098476440847548
1104	26-27	-0.3683038637439964
1104	28-29	-0.522377698737003
1104	30-31	-0.46072454148289665
1104	32-33	-0.3586632181156446
1104	34-35	-0.4186890653053297
1104	36-37	-0.41909803455133243
1104	38-39	-0.19279660697937828
1104	40-41	-0.19175014860359596
1104	42-43	-0.14726905200276974
1104	44-45	-0.2908731312589339
1104	46-47	-0.26100165649115326
1104	48-49	-0.3351122887498619
1104	50-51	-0.31466543516030754
1104	52-53	-0.3268878664187156
1104	54-55	-0.3260100020811052
1104	56-57	-0.2293207170872691
1104	58-59	-0.42668634744740785
1104	60-61	-0.39606758399947495
1104	62-63	-0.37754252745920525
1104	64-65	-0.5932123427646872
1104	66-67	-0.34979993383268493
1104	68-69	-0.30948306038911255
1104	70-71	-0.23115928807123964
1104	72-73	-0.18996284998976876
1104	74-75	-0.42985756923222596
1104	76-77	-0.3138865700727109
1104	78-79	-0.21254717408534418
1104	80-81	-0.329507439035293
1104	82-83	-0.2563852541113505
1104	84-85	-0.9614334385712873
1104	86-87	-0.30341664882707065
1104	88-89	-0.3227186099394217
1104	90-91	-0.28519472585862005
1104	92-93	-0.25455030682568847
1104	94-95	-0.2238184944576993
1104	96-97	-0.21399780012809444
1104	98-99	-0.25405242274116446
1104	100	-1.5422572324365476
1105	1	-0.050097492286688805
1105	2	-0.22554352535384936
1105	3	-0.21501195714850851
1105	4	-0.1663390552088373
1105	5	-0.1136368177116509
1105	6	-0.11197499019812085
1105	7	-0.17626830255881032
1105	8	-0.2350342226772355
1105	9	-0.28009366073938224
1105	10-11	-0.2649011506757901
1105	12-13	-0.21961283203960136
1105	14-15	-0.27843762752177525
1105	16-17	-0.3276352328028551
1105	18-19	-0.39200573135671135
1105	20-21	-0.3398048714039845
1105	22-23	-0.3405348562944184
1105	24-25	-0.31285747165969724
1105	26-27	-0.3937518991941218
1105	28-29	-0.26087123426340497
1105	30-31	-0.3037511688784704
1105	32-33	-0.2963974350486538
1105	34-35	-0.37196680880934707
1105	36-37	-0.4682384817907419
1105	38-39	-0.550018873425806
1105	40-41	-0.4356299936419745
1105	42-43	-0.4575946637894006
1105	44-45	-0.5427821244727298
1105	46-47	-0.5041133933748085
1105	48-49	-0.4525379091602204
1105	50-51	-0.28420648738688215
1105	52-53	-0.47395958260543836
1105	54-55	-1.2356583532920808
1105	56-57	-0.43948801726570963
1105	58-59	-0.36274325073570424
1105	60-61	-0.379419989022459
1105	62-63	-0.23426823344340164
1105	64-65	-0.24252954310698271
1105	66-67	-0.35553908618729935
1105	68-69	-0.08780216458355738
1105	70-71	-0.11511926737431111
1105	72-73	-0.2665402279807054
1105	74-75	-0.30188357970009605
1105	76-77	-0.2851484857327229
1105	78-79	-0.21110671424203176
1105	80-81	-0.13021809994396705
1105	82-83	-0.12213157078763714
1105	84-85	-0.13889070140239212
1105	86-87	-0.14452141376434824
1105	88-89	-0.32024257393207023
1105	90-91	-0.2586819594354104
1105	92-93	-0.2090852892797308
1105	94-95	-0.2535015395597604
1105	96-97	-0.17105614954798298
1105	98-99	-0.08544784022874197
1105	100	-0.06191361034124654
1106	1	-0.01749021067077905
1106	2	-0.08967154312098558
1106	3	-0.05661483762587949
1106	4	-0.02393846754851836
1106	5	-0.09971941242478266
1106	6	-0.08785327442888047
1106	7	-0.08313836157081766
1106	8	-0.10509921967619107
1106	9	-0.13380611758332606
1106	10-11	-0.13168300210569583
1106	12-13	-0.15887394021521573
1106	14-15	-0.22443665137636515
1106	16-17	-0.1300369832613626
1106	18-19	-0.1251765458850045
1106	20-21	-0.18794250672232238
1106	22-23	-0.09961086449241208
1106	24-25	-0.12429848181563585
1106	26-27	-0.09873574235000859
1106	28-29	-0.057118983808251755
1106	30-31	-0.06677528141944578
1106	32-33	0.046585215976008953
1106	34-35	-0.05041285793283379
1106	36-37	-0.0825146938467256
1106	38-39	-0.14377034912062214
1106	40-41	-0.11614180176553646
1106	42-43	-0.10284326846446135
1106	44-45	-0.12443388917025544
1106	46-47	-0.21336042432615443
1106	48-49	-0.18546776204389204
1106	50-51	-0.11735726206171648
1106	52-53	-0.2607514250236562
1106	54-55	-0.22519494532788542
1106	56-57	-0.21575420123959077
1106	58-59	-0.32624362055550193
1106	60-61	-0.24764751166645027
1106	62-63	-0.35671877538759844
1106	64-65	-0.2490314133836904
1106	66-67	-0.23819183400175348
1106	68-69	-0.11561030577566811
1106	70-71	-0.1477304786956566
1106	72-73	-0.09897291886803572
1106	74-75	-0.1789285720978384
1106	76-77	-0.08781643673893313
1106	78-79	-0.08014563062460667
1106	80-81	-0.057484283786308765
1106	82-83	-0.03430071425391645
1106	84-85	0.00857881164692742
1106	86-87	0.08436337463860966
1106	88-89	-0.022717324834786723
1106	90-91	0.021019806505155003
1106	92-93	0.004029952965726835
1106	94-95	-0.03627653245340312
1106	96-97	0.11920279469176265
1106	98-99	0.004441935315689705
1106	100	0.06595315087511722
1107	1	-0.026429436287063623
1107	2	-0.10546858944377391
1107	3	-0.11049193435247417
1107	4	-0.0379628708136579
1107	5	-0.1331805268158348
1107	6	-0.0911574918781426
1107	7	-0.22851085954412298
1107	8	-0.21592748016843188
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2308	82-83	0.5645044647781248
2308	84-85	0.5530363096008521
2308	86-87	0.4468878631977624
2308	88-89	0.3917468890129001
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2308	92-93	0.5286672301355999
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2309	3	0.14407459397311584
2309	4	0.15313854793715365
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2310	2	0.13210655471031885
2310	3	0.14114405527534046
2310	4	0.1771720879084242
2310	5	0.18940166870964248
2310	6	0.09919022887325468
2310	7	0.12273221266216439
2310	8	0.1716504035387274
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2310	14-15	0.26497632618445266
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2312	4	0.007455839244805418
2312	5	0.16635892198268465
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2312	9	0.21948821645409566
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2312	32-33	0.12619214380876542
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2312	36-37	-1.4426602762690521
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2312	44-45	-0.030802238848593788
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2312	56-57	0.32910522683843624
2312	58-59	0.4373162675136797
2312	60-61	0.3585545321750274
2312	62-63	0.39001358132819774
2312	64-65	0.4049514152542102
2312	66-67	0.41382664721542994
2312	68-69	0.5233595026490079
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2312	72-73	0.46421067807559524
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2312	82-83	0.36440299674932675
2312	84-85	0.2766093853358491
2312	86-87	0.24327031461991666
2312	88-89	0.33258032338221
2312	90-91	0.3874995933422163
2312	92-93	0.5246163232607906
2312	94-95	0.4049303689435071
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2313	1	0.11887292840580344
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2313	12-13	0.09745545584764415
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2313	24-25	-1.0784500401895656
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2313	60-61	-0.5088702795655635
2313	62-63	-2.210200358106654
2313	64-65	-0.7629509766734301
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2314	1	-2.089247032729922
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2314	4	-0.08863408096339498
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2314	7	0.13363173545804585
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2314	9	0.12007778785213219
2314	10-11	-0.856859652662358
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2314	64-65	0.5557097544010503
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2314	74-75	0.5640810795271918
2314	76-77	0.6040588254315615
2314	78-79	0.42596885731540013
2314	80-81	0.3810876301661281
2314	82-83	0.35374132617175746
2314	84-85	0.3408869372287029
2314	86-87	0.3420973963824281
2314	88-89	0.28133605760999814
2314	90-91	0.3532353948891931
2314	92-93	0.09762599129216198
2314	94-95	-0.04902058153484745
2314	96-97	0.17487716440420442
2314	98-99	0.13601882792877973
2314	100	0.06546747515196571
2315	1	0.1518633963117928
2315	2	0.034540963175238915
2315	3	0.040238500940901645
2315	4	-0.03542153409620141
2315	5	-0.011899053795801251
2315	6	0.01307240163642831
2315	7	-0.03826720593012567
2315	8	0.013061936209822989
2315	9	0.027364997245427958
2315	10-11	0.043397928839922884
2315	12-13	0.08000217657767195
2315	14-15	-0.010152008933282275
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2315	18-19	0.08068735950264738
2315	20-21	0.14770495836530273
2315	22-23	0.2028680230523605
2315	24-25	0.3558437514738486
2315	26-27	0.1139599901096986
2315	28-29	-1.2167714068621294
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2315	64-65	0.4863038769689254
2315	66-67	0.39952573014005566
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2315	80-81	0.38917361303816733
2315	82-83	0.4470527831321789
2315	84-85	0.3129411497093706
2315	86-87	0.36689473149716534
2315	88-89	0.3998783148183662
2315	90-91	0.41938825265966884
2315	92-93	0.544674937795854
2315	94-95	0.429847052867931
2315	96-97	0.4533996516304448
2315	98-99	0.45265028777796346
2315	100	0.3909746639628189
2316	1	0.06233835179181568
2316	2	-0.05881510262894096
2316	3	-0.02570757383791289
2316	4	-0.01690239219681189
2316	5	-0.08445245165724202
2316	6	-0.04854612899251265
2316	7	0.008032952369944724
2316	8	0.051867811924921625
2316	9	-0.10323464487479583
2316	10-11	0.05054692439929198
2316	12-13	0.042939720844259455
2316	14-15	0.1513946508862034
2316	16-17	-1.3327266203562331
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2316	20-21	0.02375748179856174
2316	22-23	0.021325337627210672
2316	24-25	0.18578215143305954
2316	26-27	0.02114188038569864
2316	28-29	0.042057860651468104
2316	30-31	0.13112583131403
2316	32-33	0.17155186817308277
2316	34-35	0.17322309170379668
2316	36-37	0.13396865056478902
2316	38-39	0.18884376385461366
2316	40-41	0.021247190170178953
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2316	46-47	0.04045371567827516
2316	48-49	0.14333380613395263
2316	50-51	0.16715678076908347
2316	52-53	0.05890662384544498
2316	54-55	0.18446766710948737
2316	56-57	0.28272968209666516
2316	58-59	0.0996582153810266
2316	60-61	-1.3165601521954144
2316	62-63	0.09311717990245683
2316	64-65	0.1841299385529922
2316	66-67	0.002205897052334649
2316	68-69	0.15615449154019245
2316	70-71	0.19490904784179008
2316	72-73	0.2910712632619763
2316	74-75	0.1969377609110765
2316	76-77	-0.14812802406117243
2316	78-79	-0.2507385378109319
2316	80-81	-0.03834068280296776
2316	82-83	-0.11143135092592615
2316	84-85	-0.7830445584423167
2316	86-87	-0.056372122361221244
2316	88-89	0.04887918771084898
2316	90-91	0.1181607360909922
2316	92-93	0.26118046846963594
2316	94-95	0.20373353138869632
2316	96-97	0.21326223095708485
2316	98-99	0.23919465940287665
2316	100	0.018508248209226252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	0.0
10	1.0
11	4.0
12	34.0
13	279.0
14	956.0
15	2384.0
16	5104.0
17	9448.0
18	15286.0
19	21813.0
20	29291.0
21	38894.0
22	48851.0
23	56605.0
24	63613.0
25	71677.0
26	83898.0
27	99380.0
28	119511.0
29	145753.0
30	177789.0
31	218372.0
32	271346.0
33	352820.0
34	505321.0
35	864786.0
36	885365.0
37	765164.0
38	609940.0
39	92477.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06510248426851	19.129760590353175	13.44126969722505	23.363867228153268
2	16.925080133974213	21.15923909443569	38.37565986331866	23.54002090827144
3	19.256706350341936	26.46493846771591	30.016599507324177	24.26175567461798
4	13.506639734450218	17.124328966837055	36.53413474855255	32.834896550160174
5	13.834131454917426	36.653983471711	33.81476200066557	15.697123072706006
6	30.614797300811762	35.85702358217116	18.684479595636503	14.843699521380572
7	27.54744304054452	32.26114158463652	22.064566198162087	18.12684917665688
8	26.691795428624292	34.23989094671648	20.396952368199535	18.671361256459697
9	27.195367184233653	15.763772614646479	20.30996425787532	36.730895943244555
10-11	25.20228343564267	25.75473044563459	28.216381768150793	20.826604350571948
12-13	25.633338189792276	23.668596081710536	28.265562933633564	22.432502794863623
14-15	23.20506781511896	25.01128107064245	26.313531506990845	25.47011960724775
16-17	22.503354873637893	27.558829077107376	27.095239175133045	22.842576874121686
18-19	22.25810115068142	26.598551596364846	29.07164195007151	22.07170530288223
20-21	23.067014426113673	25.881396606402703	29.073748058341586	21.977840909142042
22-23	22.925029946499905	25.674384559043805	28.862450558240916	22.538134936215375
24-25	22.595960576796134	25.883255415596906	28.87092641757289	22.649857590034063
26-27	22.53343579314279	25.78753378050596	28.996900104745894	22.682130321605356
28-29	22.542201529273026	26.115956296044896	29.00035109253138	22.341491082150704
30-31	23.162615649596997	25.86167173325044	28.914878692682787	22.060833924469772
32-33	22.835061775281698	26.088432450820736	28.788779699135457	22.28772607476211
34-35	22.996817061063883	26.13502727686961	28.69788395354009	22.170271708526418
36-37	23.032802516158185	26.081310772335975	28.564783162784344	22.321103548721496
38-39	22.94999645426196	26.307756382958424	28.43675609245989	22.305491070319725
40-41	22.897449551289533	26.097203037042565	28.58949699196282	22.415850419705087
42-43	22.759899499821	26.325670545668313	28.293448067257863	22.620981887252825
44-45	22.728372311691995	26.358764811950287	28.285483793745588	22.627379082612137
46-47	22.796620512157727	26.16292298049573	28.051275534174145	22.9891809731724
48-49	22.76580103115906	26.331448767321742	28.057403539897564	22.845346661621637
50-51	22.721790673691526	26.643763083186794	27.908245380056616	22.72620086306506
52-53	22.757900635096377	26.776970716195432	27.504901123881275	22.960227524826916
54-55	22.67366365998018	26.54005652082238	27.814839276167024	22.97144054303042
56-57	22.787956694611918	26.675036622682253	27.458570337598225	23.078436345107608
58-59	22.77672558846802	26.577656971005176	27.79513760140197	22.850479839124834
60-61	23.06688967845734	26.657204324144146	27.768531615664987	22.507374381733527
62-63	22.839429514830595	26.66853305587667	27.77497609419992	22.717061335092815
64-65	22.73271630213231	26.467767843885408	27.72113909299113	23.078376760991148
66-67	22.757193750627682	26.53054943520162	27.50151094050512	23.210745873665577
68-69	22.49129573619309	26.525547655450637	27.65871726733361	23.32443934102266
70-71	22.543716202036723	26.389084366987525	27.687818856775287	23.379380574200468
72-73	22.473521077936503	26.724187426869904	27.29756518286551	23.50472631232808
74-75	22.599634909584147	26.765866601966493	27.155336576501597	23.479161911947767
76-77	22.43595189403989	26.54993734590711	27.392923777273246	23.621186982779754
78-79	22.33329331654188	26.554080893793934	27.441040777350473	23.671585012313713
80-81	22.408670047342724	26.77453321626263	27.073992206705082	23.74280452968956
82-83	22.52123168569411	26.647350503942352	27.175387811847486	23.656029998516058
84-85	22.471101516979132	26.830882186195034	26.93932011488481	23.758696181941026
86-87	22.27948887822207	26.784596802366234	27.14389099975505	23.79202331965665
88-89	22.172332820810848	26.51082695499911	27.236820484005285	24.080019740184756
90-91	22.532138805985596	26.49355289095146	27.140149086443948	23.834159216618993
92-93	22.357720284915793	26.52757260039653	27.092152361123713	24.02255475356397
94-95	22.116017312589133	26.37882303478572	27.153337864017562	24.351821788607587
96-97	22.04619842502792	26.351863632367206	27.285837355115284	24.316100587489583
98-99	22.108182722091833	26.328801100111416	27.0234889553756	24.53952722242115
100	21.930361185007765	26.38230238276889	27.15227342223969	24.535063009983652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3240.0
1	2394.5
2	4033.0
3	5605.5
4	3380.0
5	2232.0
6	3136.5
7	5555.5
8	8089.0
9	9216.5
10	8047.5
11	6262.0
12	6104.5
13	6535.5
14	7055.5
15	7524.0
16	8010.5
17	8567.5
18	9210.0
19	9966.5
20	10575.5
21	11418.0
22	12954.5
23	15222.0
24	17828.5
25	21086.0
26	25871.0
27	31513.5
28	37135.5
29	44098.5
30	52548.5
31	60625.0
32	69916.0
33	80206.0
34	90121.0
35	99783.5
36	112003.5
37	124575.5
38	133395.5
39	141680.5
40	148257.5
41	153672.0
42	160129.5
43	168256.5
44	177190.5
45	186680.5
46	195925.0
47	206017.5
48	218571.5
49	226866.0
50	233074.5
51	230760.5
52	219177.0
53	211630.0
54	205181.0
55	193197.0
56	176837.0
57	161081.5
58	146125.0
59	128148.5
60	106899.0
61	87162.0
62	71027.0
63	55874.0
64	41723.5
65	31261.5
66	23783.5
67	18333.0
68	13996.5
69	10711.5
70	8424.5
71	6448.5
72	5060.5
73	4018.5
74	3015.5
75	2157.0
76	1542.5
77	1007.5
78	624.0
79	416.5
80	274.0
81	173.5
82	118.5
83	82.5
84	49.5
85	32.0
86	30.5
87	28.0
88	15.0
89	5.5
90	2.0
91	2.5
92	2.5
93	1.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04249315493047483
2	0.008872988301873821
3	0.0
4	3.239630617317013E-4
5	3.239630617317013E-4
6	0.0
7	0.0
8	1.0798768724390044E-4
9	0.0
10-11	0.0
12-13	2.609702441727594E-4
14-15	0.007595134002820998
16-17	0.0016738091522804572
18-19	5.399384362195022E-5
20-21	8.908984197621788E-4
22-23	5.48937410156494E-4
24-25	0.004004543401961308
26-27	0.015118276214146062
28-29	0.012121617893127825
30-31	0.009106961624235604
32-33	0.006596247895814918
34-35	0.008540026266205128
36-37	0.004652469525424711
38-39	0.0040135423758983005
40-41	0.0058223361372336324
42-43	0.006803224296365728
44-45	0.006677238661247844
46-47	0.014236376768320876
48-49	0.015964179764223284
50-51	0.015694210546113533
52-53	0.010357819001477453
54-55	0.0033026234348759553
56-57	0.0037075772620405825
58-59	0.010384815923288427
60-61	0.002339733223617843
62-63	1.0798768724390044E-4
64-65	2.519712702357677E-4
66-67	8.459035500772202E-4
68-69	7.199179149593364E-5
70-71	1.979774266138175E-4
72-73	0.002987659347081246
74-75	0.00548937410156494
76-77	0.005165411039833238
78-79	0.0033836142003088807
80-81	4.4994869684958526E-5
82-83	8.549025240142118E-4
84-85	2.6996921810975113E-4
86-87	3.239630617317013E-4
88-89	1.799794787398341E-4
90-91	2.969661399207263E-4
92-93	3.7795690535365157E-4
94-95	1.799794787398341E-5
96-97	0.0016288142825954986
98-99	4.409497229125935E-4
100	1.0798768724390044E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5556189.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.67581921864484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18785377251497	44.11708915393992
2	14.967555307107247	17.564671386897864
3	4.634603076058426	8.158173967229384
4	1.881264322481014	4.415389011535295
5	0.9607231386633484	2.8185608601689847
6	0.5435556519515005	1.9136143901507328
7	0.3718870121482542	1.5274542566201061
8	0.26276203485279337	1.233422212363661
9	0.1897542119664527	1.0020585453587993
>10	0.9176331829467764	9.504378642847728
>50	0.05439753377614486	2.193694297930677
>100	0.0256522684252545	2.6792892363696628
>500	0.0014074845896746024	0.5539018484377819
>1k	7.988421152329761E-4	0.9627432568020964
>5k	3.804010072533983E-5	0.18485706194400423
>10k+	1.1412030217601948E-4	1.1707018714034076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20780	0.37399735682137525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19388	0.34894421338079035	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12282	0.22105079578826423	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8282	0.1490590042923306	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.799794787398341E-5
2	0.0	0.0	0.0	0.0	1.799794787398341E-5
3	0.0	0.0	0.0	0.0	1.799794787398341E-5
4	0.0	0.0	0.0	0.0	5.399384362195023E-5
5	0.0	0.0	0.0	0.0	5.399384362195023E-5
6	1.799794787398341E-5	0.0	0.0	0.0	1.0798768724390045E-4
7	1.799794787398341E-5	0.0	0.0	0.0	1.0798768724390045E-4
8	1.799794787398341E-5	0.0	0.0	0.0	1.619815308658507E-4
9	1.799794787398341E-5	0.0	0.0	0.0	2.339733223617843E-4
10-11	1.799794787398341E-5	0.0	0.0	0.0	2.339733223617843E-4
12-13	1.799794787398341E-5	8.998973936991705E-6	0.0	0.0	2.339733223617843E-4
14-15	1.799794787398341E-5	1.799794787398341E-5	0.0	1.799794787398341E-5	2.969661399207262E-4
16-17	1.799794787398341E-5	1.799794787398341E-5	0.0	3.599589574796682E-5	4.679466447235686E-4
18-19	1.799794787398341E-5	1.799794787398341E-5	0.0	4.4994869684958526E-5	5.399384362195023E-4
20-21	1.799794787398341E-5	1.799794787398341E-5	0.0	1.1698666118089215E-4	5.579363840934856E-4
22-23	1.799794787398341E-5	1.799794787398341E-5	0.0	3.7795690535365157E-4	5.849333059044608E-4
24-25	1.799794787398341E-5	1.799794787398341E-5	0.0	8.549025240142118E-4	6.119302277154359E-4
26-27	1.799794787398341E-5	1.799794787398341E-5	0.0	0.001799794787398341	6.479261234634028E-4
28-29	1.799794787398341E-5	1.799794787398341E-5	0.0	0.004670467473298695	6.659240713373861E-4
30-31	1.799794787398341E-5	1.799794787398341E-5	0.0	0.012310596345804652	6.659240713373861E-4
32-33	1.799794787398341E-5	1.799794787398341E-5	0.0	0.030155561662859202	6.659240713373861E-4
34-35	1.799794787398341E-5	2.6996921810975114E-5	1.799794787398341E-5	0.05962720130650703	6.929209931483612E-4
36-37	1.799794787398341E-5	4.4994869684958526E-5	1.799794787398341E-5	0.10601691195169927	7.109189410223446E-4
38-39	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.17558797945858212	7.379158628333198E-4
40-41	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.25809057251292206	7.559138107073031E-4
42-43	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.33667861190467063	7.649127846442948E-4
44-45	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.43446146270402247	7.739117585812866E-4
46-47	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.551070166979561	7.9190970645527E-4
48-49	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.6754539847366603	8.279056022032368E-4
50-51	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.8054261653086315	8.279056022032368E-4
52-53	1.799794787398341E-5	5.399384362195023E-5	1.799794787398341E-5	0.9387369652112266	8.81899445825187E-4
54-55	1.799794787398341E-5	6.299281755894193E-5	1.799794787398341E-5	1.0994586397258985	9.538912373211206E-4
56-57	1.799794787398341E-5	7.199179149593364E-5	1.799794787398341E-5	1.2787271995247103	9.898871330690875E-4
58-59	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	1.4831658894252877	0.0010258830288170542
60-61	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	1.691141176083103	0.0010258830288170542
62-63	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	1.9017261651826458	0.0010618789245650212
64-65	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	2.125161689064213	0.0010618789245650212
66-67	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	2.3813894739721775	0.0010708778985020128
68-69	1.799794787398341E-5	8.998973936991704E-5	1.799794787398341E-5	2.6775547052125117	0.0010798768724390045
70-71	1.799794787398341E-5	1.0798768724390045E-4	1.799794787398341E-5	2.9966313240964264	0.001124871742123963
72-73	1.799794787398341E-5	1.0798768724390045E-4	1.799794787398341E-5	3.296999076165336	0.0012418584033048552
74-75	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	3.6017943234112444	0.0012598563511788386
76-77	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	3.940083751650637	0.0012688553251158302
78-79	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	4.332717983495522	0.001277854299052822
80-81	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	4.72627011068198	0.0013138501948007889
82-83	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	5.128335267212832	0.0013948409602337142
84-85	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	5.5436289154310625	0.001403839934170706
86-87	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	5.979170255007524	0.001430836855981681
88	3.599589574796682E-5	1.0798768724390045E-4	1.799794787398341E-5	6.320807301551477	0.0014398358299186726
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	9265	0.0	50.523815	2
GTACCTG	10415	0.0	45.59737	1
ACCTGGG	10870	0.0	42.455395	3
CCTGGGG	9385	0.0	32.64858	4
GGTATCA	18770	0.0	30.386045	1
GTATCAA	26145	0.0	26.79633	1
TATAACG	685	0.0	24.69984	2
GTACCCG	955	0.0	24.617523	1
TAACGCA	745	0.0	24.60137	4
CTGGGGG	5845	0.0	24.442184	5
TATCACG	480	0.0	22.520021	2
TCAACGC	31265	0.0	22.170961	4
CAACGCA	31535	0.0	21.99604	5
ATCAACG	31550	0.0	21.925999	3
TATCAAC	31800	0.0	21.902985	2
ATAACGC	760	0.0	21.6424	3
AACGCAG	32495	0.0	21.505293	6
GTACATG	23695	0.0	21.4708	1
TACATGG	24370	0.0	20.69314	2
GAGTACT	20320	0.0	20.113276	12-13
>>END_MODULE