FastQCFastQC Report
Mon 6 Jun 2016
ERR523062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6903748
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT221710.3211443986657682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210140.30438538602509824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172790.2502843383043529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97090.14063375430273525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG214100.025.8499981
TACATGG215900.024.7190232
ACATGGG215150.023.8664783
CATGGGG136000.021.7457584
GAGTACT201550.020.31576512-13
AGAGTAC288300.019.6683810-11
GTATCAA461400.018.8434331
GTACTTT217100.018.514714-15
AGTACTT218950.017.59614412-13
TCAACGC486450.017.4269084
CAACGCA489900.017.2756425
ATCAACG492700.017.2171383
TACCTGG45400.017.0889822
AACGCAG499500.016.977596
TATCAAC504850.016.9045012
GTATAGG18500.016.5212291
GTGTAGC43250.016.3081931
GAGTACA159100.016.284791
ATGGGGG77000.016.1817055
ACGCAGA539100.015.7041167
Produced by FastQC (version 0.11.5)