FastQCFastQC Report
Mon 6 Jun 2016
ERR523062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6903748
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT223880.3242876188412439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189820.2749520984833166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163300.23653818186874725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88310.12791602474481978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG218000.022.459391
TACATGG219250.021.8230842
ACATGGG214350.021.4202983
GAGTACT200250.020.43834712-13
GTACTTT212650.019.06566614-15
CATGGGG139150.018.8791774
AGAGTAC294650.018.5894210-11
GTATCAA449550.017.9637091
TACCTGG47700.017.539192
GAGTACA165900.017.3787211
AGTACTT218400.016.72699412-13
TCAACGC476850.016.7048424
CAACGCA480750.016.5496565
ATCAACG481350.016.4900933
AACGCAG490450.016.270256
GGTATCA360300.016.1737521
TATCAAC497550.016.059062
AGTACAT169150.015.8661672
ACTTTTT257750.015.74462316-17
GTGTAGC43000.015.2037291
Produced by FastQC (version 0.11.5)