FastQCFastQC Report
Mon 6 Jun 2016
ERR523061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9360909
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263320.28129746801298894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213960.22856754616458722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114260.12206079559153925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244900.031.761931
GTATCAA367450.031.2285441
TCAACGC421450.026.8920674
CAACGCA425200.026.6088055
ATCAACG431000.026.2525523
GTACATG362500.026.050491
AACGCAG438400.025.9478056
TATCAAC444700.025.623972
TACATGG371050.024.8164942
ACATGGG372700.023.9112933
ACGCAGA492700.022.9524697
CATGGGG223450.022.4983444
CGCAGAG502350.022.4455838
GAGTACT253550.020.92885812-13
TACCTGG82750.020.686812
GCAGAGT542550.020.4787359
GTACTTT265650.019.7814814-15
AGAGTAC435200.019.2282810-11
GTATAGG25950.019.028831
AGTACTT270000.018.052212-13
Produced by FastQC (version 0.11.5)