FastQCFastQC Report
Mon 6 Jun 2016
ERR523061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9360909
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242000.25852190209305526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212370.22686899317149647No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT174440.1863494239715395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113380.12112071594756449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244150.033.5744741
GTATCAA356800.029.3010481
TCAACGC407350.025.3618074
GTACATG353100.024.9997181
CAACGCA412850.024.9674115
ATCAACG414950.024.863313
AACGCAG425950.024.263166
TACATGG364400.024.005412
TATCAAC433750.023.8518282
ACATGGG367150.023.1459563
ACGCAGA483550.021.3341927
CATGGGG220600.020.7952694
CGCAGAG494900.020.7021378
GAGTACT248750.020.48025512-13
TACCTGG83950.019.8209132
GTACTTT254450.019.78125614-15
GCAGAGT538550.018.7623089
TATAACG7300.018.0291922
AGAGTAC430050.017.84600810-11
AGTACTT261250.017.3596212-13
Produced by FastQC (version 0.11.5)