FastQCFastQC Report
Mon 6 Jun 2016
ERR523060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9426805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377620.40058110886986625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301950.3203100095949794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199700.21184271871540783No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160660.17042889929302665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG172250.056.9522862
GTACCTG199350.049.575831
ACCTGGG195750.049.3941043
TATAACG9150.037.5195732
CCTGGGG177650.037.506794
GGTATCA255700.033.021281
GTATCAA380750.031.170051
TAACGCA11750.029.2139474
ATAACGC12200.028.5250023
CTGGGGG110250.027.0816365
TATCACG9200.026.0698472
TCAACGC455000.025.629824
CATGGGG259950.025.2342054
CAACGCA461300.025.2171545
ATCAACG461700.025.1995983
TATCAAC466500.025.021092
GTACATG425650.025.0087551
AACGCAG475100.024.7080026
ATGGGGG165600.024.645595
TGGGGGG182000.024.2451236
Produced by FastQC (version 0.11.5)