FastQCFastQC Report
Mon 6 Jun 2016
ERR523060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9426805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332530.352749420402777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290550.3082168348661079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192000.20367452174941564No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135460.14369661831341585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG175300.057.5080262
GTACCTG203450.050.0342831
ACCTGGG202550.048.954153
CCTGGGG180450.037.0584564
GGTATCA257850.033.1143461
TATAACG10300.032.8532522
GTATCAA372450.029.4140871
CTGGGGG108600.028.9057125
TAACGCA12000.026.6297444
TATCACG8850.026.552772
TGGGGGG165100.025.7309656
ATAACGC13100.025.111123
TCAACGC447800.024.1789634
GTACATG412000.024.1254391
CATGGGG254100.024.0054234
CAACGCA452000.023.9229775
ATGGGGG162550.023.8219475
ATCAACG456650.023.7103713
TATCAAC459250.023.7013172
TACATGG420500.023.3259622
Produced by FastQC (version 0.11.5)