FastQCFastQC Report
Mon 6 Jun 2016
ERR523059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545528
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391310.4099406549328649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305320.31985658624646013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207340.21721166183787843No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177720.18618142443246724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG201700.059.778442
ACCTGGG223300.053.110973
GTACCTG234550.051.9158361
CCTGGGG189300.039.518414
TATAACG9300.030.3389642
GGTATCA287100.029.1437871
GTATCAA415400.028.8833261
CTGGGGG111650.028.3405885
GTACCCG19150.026.0339681
CATGGGG225750.025.1188344
TATCACG10150.024.555132
TCAACGC485600.024.4878184
CAACGCA489850.024.2642295
ATCAACG493100.024.1370943
GTACATG419600.023.8976361
TGGGGGG161000.023.8762136
TATCAAC496650.023.8606642
TACCGGG12450.023.7959822
AACGCAG505250.023.7641646
ATGGGGG142100.023.6241765
Produced by FastQC (version 0.11.5)