##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523059_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9545528
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45967829123753	33.0	31.0	34.0	28.0	34.0
2	31.789757675007607	34.0	31.0	34.0	28.0	35.0
3	31.78742663580265	34.0	31.0	34.0	27.0	36.0
4	34.09178727462745	35.0	34.0	37.0	32.0	37.0
5	34.186524726552584	35.0	34.0	37.0	31.0	37.0
6	34.41127468276244	35.0	35.0	37.0	30.0	37.0
7	34.30397218467119	35.0	34.0	37.0	31.0	37.0
8	34.41370325455019	36.0	34.0	37.0	30.0	37.0
9	35.60110284103718	37.0	35.0	39.0	31.0	39.0
10-11	35.563764833123955	37.0	34.5	39.0	30.0	39.0
12-13	35.594545058167554	37.5	35.0	39.0	30.5	39.0
14-15	36.38148649294203	38.0	35.0	41.0	31.0	41.0
16-17	36.53946617725075	38.0	36.0	41.0	31.0	41.0
18-19	36.4076029110176	38.0	35.5	41.0	30.0	41.0
20-21	36.50619049045794	38.0	35.5	41.0	30.0	41.0
22-23	36.34704821985751	38.0	35.5	41.0	30.0	41.0
24-25	36.32724632938063	38.0	35.0	40.5	30.0	41.0
26-27	36.20956336831236	38.0	36.0	40.0	29.5	41.0
28-29	35.978892210048514	38.0	35.0	40.0	29.0	41.0
30-31	35.788965209677244	37.0	35.5	40.0	29.0	41.0
32-33	35.75405147834672	37.5	34.5	40.0	27.5	41.0
34-35	35.59252531656709	37.5	34.5	40.0	27.5	41.0
36-37	35.408021693509255	37.5	34.5	40.0	27.5	41.0
38-39	35.23207301890477	36.5	35.0	40.0	27.0	41.0
40-41	35.17553251114029	37.0	34.0	40.0	26.5	41.0
42-43	34.96307663651503	36.0	35.0	40.0	26.0	41.0
44-45	34.88267977423564	37.0	34.0	40.0	26.0	41.0
46-47	34.655863719639186	36.5	34.0	40.0	24.0	41.0
48-49	34.515458757231656	37.0	33.5	40.0	24.0	41.0
50-51	33.48104133160575	36.5	32.0	38.5	23.0	40.0
52-53	33.69118460497942	36.5	33.0	38.5	23.0	39.5
54-55	34.534487667942514	37.5	34.0	39.5	24.0	41.0
56-57	34.66850655092102	37.0	34.0	40.0	24.5	41.0
58-59	34.60402572806868	37.0	34.0	40.0	24.0	41.0
60-61	34.35345488484241	36.5	34.0	40.0	24.0	41.0
62-63	34.18521966516677	37.0	33.5	39.0	23.5	41.0
64-65	33.790316784990836	36.0	33.0	39.0	23.0	41.0
66-67	33.59756867299535	36.0	33.0	39.0	22.5	41.0
68-69	33.26395794973311	35.5	33.0	38.0	22.0	40.0
70-71	33.06393522705082	35.5	32.0	37.5	21.5	40.0
72-73	32.741006835871204	35.0	32.0	37.5	21.0	39.0
74-75	32.36216566542993	35.0	32.0	37.0	20.5	39.0
76-77	32.24267683254399	35.0	31.0	37.5	19.5	39.0
78-79	31.96099477158309	35.0	31.0	37.0	20.0	38.0
80-81	31.70057999934629	35.0	31.0	36.5	18.5	37.5
82-83	31.386520001827034	35.0	31.0	36.0	18.0	37.0
84-85	31.142140801430784	35.0	31.0	35.5	18.0	37.0
86-87	30.89971764788705	34.0	30.0	35.5	17.5	37.0
88-89	30.741139411041484	34.5	30.0	35.0	17.5	36.5
90-91	30.530771215589123	34.0	29.5	35.0	17.0	36.0
92-93	30.272152467626725	34.0	29.0	35.0	17.0	36.0
94-95	30.172762365790557	34.0	29.0	35.0	16.0	36.0
96-97	29.98310496810653	34.0	29.0	35.0	17.0	36.0
98-99	29.888746541836134	34.0	29.0	35.0	15.5	36.0
100	28.660587449955624	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08437675632525554
1101	2	0.014946983397368996
1101	3	0.0985638623505416
1101	4	0.11593379604057219
1101	5	0.04190224241177276
1101	6	0.186054964339597
1101	7	0.1890421341363009
1101	8	0.18343441051453624
1101	9	0.15930345935325363
1101	10-11	0.1386485998579161
1101	12-13	0.16042711459681414
1101	14-15	0.05622415659260582
1101	16-17	0.06200535282793851
1101	18-19	0.10207459112103834
1101	20-21	0.09951904115641241
1101	22-23	0.12561460103968614
1101	24-25	0.06658839998443256
1101	26-27	0.07622077439301478
1101	28-29	0.06103098086836667
1101	30-31	9.125769143381035E-4
1101	32-33	0.008680860446197869
1101	34-35	-0.00877306422010804
1101	36-37	-0.05316145229286917
1101	38-39	-0.09851834014024519
1101	40-41	-0.08985523828354047
1101	42-43	-0.09400292915502462
1101	44-45	-0.09561121519713822
1101	46-47	-0.1001320358139921
1101	48-49	-0.1278733505115568
1101	50-51	-0.16306064218292704
1101	52-53	-0.09384827351460245
1101	54-55	0.018710585180990336
1101	56-57	-0.06701567429519173
1101	58-59	-0.024186373121956706
1101	60-61	-0.05434093468139878
1101	62-63	-0.12999654808336203
1101	64-65	-0.085677950800914
1101	66-67	-0.16871280275847766
1101	68-69	-0.0887391134522133
1101	70-71	-0.20738532816678656
1101	72-73	-0.17548054219319198
1101	74-75	-0.21625714658490125
1101	76-77	-0.1322057934962828
1101	78-79	-0.20566741580094572
1101	80-81	-0.17860928310152246
1101	82-83	-0.36148799909283014
1101	84-85	-0.2562720427675984
1101	86-87	-0.233912514764544
1101	88-89	-0.2922436267107109
1101	90-91	-0.2772978777094899
1101	92-93	-0.3619294276424405
1101	94-95	-0.3304457659862692
1101	96-97	-0.3324591302816522
1101	98-99	-0.2408356981444122
1101	100	-0.3572322421170071
1102	1	0.01214585944061497
1102	2	0.008492133368605437
1102	3	0.051401404307966914
1102	4	0.013712670307725716
1102	5	0.014636275264749088
1102	6	0.05420147079200177
1102	7	0.04685030878085428
1102	8	0.1096192473790012
1102	9	0.036675528135525326
1102	10-11	0.004500432407198218
1102	12-13	-0.07222104207051672
1102	14-15	-0.13476320363680827
1102	16-17	-0.05171723629497649
1102	18-19	0.04235543142439724
1102	20-21	-0.08945971404882869
1102	22-23	-0.0772098807733741
1102	24-25	-0.10431209560196919
1102	26-27	-0.0842655073906613
1102	28-29	-0.054672910027434796
1102	30-31	-0.19419296129783703
1102	32-33	-0.19978223317897204
1102	34-35	-0.22393271862954123
1102	36-37	-0.5460253228355327
1102	38-39	-0.28729551020420274
1102	40-41	-0.877750771918663
1102	42-43	-0.9135582084719758
1102	44-45	-0.3145463035095162
1102	46-47	-0.2636170682227714
1102	48-49	-0.382739279718173
1102	50-51	-0.3905847103585671
1102	52-53	-0.2816657161654632
1102	54-55	-0.16717313575864523
1102	56-57	-0.04137874169544631
1102	58-59	-0.0055372775984778855
1102	60-61	-0.16681315616451542
1102	62-63	-0.3325883310566482
1102	64-65	-0.1534055470298057
1102	66-67	-0.1350252333693973
1102	68-69	-0.10831434976354615
1102	70-71	-0.2384250706913562
1102	72-73	-0.19616463053365152
1102	74-75	-0.1329544454426852
1102	76-77	-0.2974967638824886
1102	78-79	-0.21728154597119698
1102	80-81	-0.3917831000575305
1102	82-83	-0.4005545586532371
1102	84-85	-0.42563706485000097
1102	86-87	-0.355770521641233
1102	88-89	-0.38330538517895363
1102	90-91	-0.3963417831208993
1102	92-93	-0.45815937116626415
1102	94-95	-0.4798348306233642
1102	96-97	-0.634333340059694
1102	98-99	-0.6256346317232868
1102	100	-0.7136073319359646
1103	1	-0.017800046398587455
1103	2	-0.040521184790797804
1103	3	0.032327100175017165
1103	4	0.020602679484468922
1103	5	0.07465899555282363
1103	6	0.09962782138126158
1103	7	0.12143043673446385
1103	8	0.07710937219965075
1103	9	0.07273061390515068
1103	10-11	0.04445207983113875
1103	12-13	0.10578655038996487
1103	14-15	0.057746548517130236
1103	16-17	-0.01761018052396679
1103	18-19	-0.03169824325551929
1103	20-21	-0.09468824691805366
1103	22-23	-0.02029148616681198
1103	24-25	-0.05530566925532554
1103	26-27	-0.09380249223811177
1103	28-29	-0.24375815386801492
1103	30-31	-0.24059978870737808
1103	32-33	-0.23954242078917076
1103	34-35	-0.2889513342877166
1103	36-37	-0.3673741401729913
1103	38-39	-0.33336340256244057
1103	40-41	-0.1916077289408733
1103	42-43	-0.3215841603871965
1103	44-45	-0.24259552753559177
1103	46-47	-0.22090296247370134
1103	48-49	-0.2636872276600286
1103	50-51	-0.3759328538033486
1103	52-53	-0.1947995643661855
1103	54-55	-0.1582141016730816
1103	56-57	0.01637913423114412
1103	58-59	-0.01654592126942589
1103	60-61	0.00425479292875508
1103	62-63	-0.09993316045919443
1103	64-65	-0.05419488653016913
1103	66-67	-0.02406022192026569
1103	68-69	-0.13152114965360795
1103	70-71	-0.1210551644068758
1103	72-73	-0.11784521639268064
1103	74-75	-0.06110285424018258
1103	76-77	-0.016111920400092572
1103	78-79	-0.12203188617233707
1103	80-81	-0.06116168309844028
1103	82-83	-0.09851875060968851
1103	84-85	-0.11802415587555615
1103	86-87	-0.782382629517091
1103	88-89	-0.2666760143274054
1103	90-91	-0.5125136486868307
1103	92-93	-0.21317393534629758
1103	94-95	-0.46837797426701755
1103	96-97	-0.275478919391805
1103	98-99	-0.26698296412556743
1103	100	-0.3484930669943793
1104	1	0.07275374728113704
1104	2	0.012600104478810437
1104	3	0.10344290291203606
1104	4	0.010136696069920959
1104	5	0.005783262762712127
1104	6	0.07897384014111708
1104	7	-0.011000925293615182
1104	8	0.05247091496007528
1104	9	0.12411077469199228
1104	10-11	-0.08964900733278824
1104	12-13	0.0164950799255692
1104	14-15	-0.1289233071255893
1104	16-17	-0.06630487347110403
1104	18-19	0.0665951619284968
1104	20-21	-0.024204037831992764
1104	22-23	-0.10471708849176053
1104	24-25	-0.0265892060939521
1104	26-27	-0.04929633678333545
1104	28-29	-0.1127847382958862
1104	30-31	-0.04319602917992427
1104	32-33	-0.08101380885664611
1104	34-35	-0.09414172950661737
1104	36-37	-0.10076224269013068
1104	38-39	-0.08924165836615572
1104	40-41	-0.02182278042314323
1104	42-43	-0.22106648910169469
1104	44-45	-0.15119240230728082
1104	46-47	-0.01792122616503633
1104	48-49	-0.10393715486818422
1104	50-51	-0.2745530386511348
1104	52-53	-0.16584791471765215
1104	54-55	-0.02471118290782215
1104	56-57	-0.06530732557995123
1104	58-59	-0.08400990941868258
1104	60-61	-0.21203020570598596
1104	62-63	-0.16916779309666907
1104	64-65	-0.15226901037917173
1104	66-67	-0.025582313395929646
1104	68-69	-0.05578161371219892
1104	70-71	-0.039107851757826495
1104	72-73	0.004681311958954382
1104	74-75	-0.0015842970573700654
1104	76-77	-0.05153805154603219
1104	78-79	-0.08323448849120751
1104	80-81	-0.037661257669888926
1104	82-83	0.012753914232106922
1104	84-85	-0.10186101132508085
1104	86-87	-0.08596103250156162
1104	88-89	-0.07111386761217986
1104	90-91	-0.2597323327890564
1104	92-93	-0.1959816875257907
1104	94-95	-0.16305199492619593
1104	96-97	-0.1760232402990205
1104	98-99	-0.23717528476771577
1104	100	-0.04898939832116156
1105	1	0.08903754350193083
1105	2	-0.007920587882949803
1105	3	0.025354122058562467
1105	4	0.1009502268844713
1105	5	0.06909765872823215
1105	6	0.14186094678528605
1105	7	-0.04018835191977388
1105	8	-0.0015333703082234251
1105	9	0.013918629878943989
1105	10-11	0.04930144876232845
1105	12-13	0.11036834445669541
1105	14-15	0.08583552400305905
1105	16-17	0.01505138898554037
1105	18-19	0.026925300559440757
1105	20-21	-0.02816673979388895
1105	22-23	0.046307745254679844
1105	24-25	0.0741955545110855
1105	26-27	0.01972362425050278
1105	28-29	-0.11275739447764721
1105	30-31	-0.03502419360115283
1105	32-33	-0.09913953864059266
1105	34-35	-0.06046020503954708
1105	36-37	-0.10975711440412539
1105	38-39	-0.07984583509193754
1105	40-41	-0.11509881921084997
1105	42-43	-0.08857042441331942
1105	44-45	-0.12171825521865998
1105	46-47	-0.033851559047583635
1105	48-49	-0.032035776472234545
1105	50-51	-0.17030810452088474
1105	52-53	-0.07019338244895579
1105	54-55	3.784916732882948E-4
1105	56-57	0.049203031539619246
1105	58-59	0.011758743455949627
1105	60-61	-0.03183836887435376
1105	62-63	0.010772512010099433
1105	64-65	0.001632861764022664
1105	66-67	0.0707606119999653
1105	68-69	0.161883704791407
1105	70-71	0.09680135764713071
1105	72-73	0.09900872113950498
1105	74-75	0.13406840849074086
1105	76-77	0.07886255900153571
1105	78-79	0.08042101433764515
1105	80-81	0.058939869667703704
1105	82-83	0.049855713045946715
1105	84-85	-0.09416226102014846
1105	86-87	-0.036615408104484715
1105	88-89	-0.09943159566212145
1105	90-91	-0.14377327086974034
1105	92-93	-0.02332443341319035
1105	94-95	-0.04585367999393952
1105	96-97	-0.09857708569704826
1105	98-99	-0.0861162450793529
1105	100	-0.003915864618004861
1106	1	0.016725373890725592
1106	2	0.0026974690529897316
1106	3	0.06566425951482202
1106	4	0.07455455810467271
1106	5	0.06609552658226647
1106	6	0.11152578496572829
1106	7	0.04796703420009152
1106	8	-1.0968767683374097
1106	9	0.07405272924115991
1106	10-11	0.033977020955020976
1106	12-13	0.03572509532947521
1106	14-15	0.0471606721651483
1106	16-17	-0.037680262872839876
1106	18-19	0.07910599274018182
1106	20-21	0.040309597559577526
1106	22-23	0.00703272178486003
1106	24-25	0.008659617332106961
1106	26-27	0.057669406341254614
1106	28-29	-0.08479789606256816
1106	30-31	0.05215634485563925
1106	32-33	-0.05991628073503108
1106	34-35	-0.005095211925450371
1106	36-37	0.0932612672698383
1106	38-39	-0.02981424530150889
1106	40-41	0.011364223158302877
1106	42-43	0.01837582771079127
1106	44-45	-0.04922849415188324
1106	46-47	-0.07195041117339684
1106	48-49	-0.13815253940436634
1106	50-51	-0.0623558991708677
1106	52-53	-0.07990818299549574
1106	54-55	-0.01915075658079246
1106	56-57	-0.04766635985484413
1106	58-59	-0.012054058734619844
1106	60-61	0.06325217666817906
1106	62-63	-0.014133353638825952
1106	64-65	-0.007727392864602223
1106	66-67	0.008123653524677366
1106	68-69	0.04127497963706617
1106	70-71	-0.015933917067918912
1106	72-73	-0.05206456001517523
1106	74-75	0.04724915229611071
1106	76-77	0.003160852622968946
1106	78-79	8.377700283475065E-4
1106	80-81	-0.012312410866680779
1106	82-83	0.02238562836158664
1106	84-85	0.05911860752202713
1106	86-87	-0.02809958664157719
1106	88-89	-0.016447207779744843
1106	90-91	-0.03734946593019117
1106	92-93	-0.026882959409068263
1106	94-95	-0.08986227338201047
1106	96-97	-0.06710856073287275
1106	98-99	-0.05160058693474667
1106	100	-0.04929893717815048
1107	1	-0.06854882845118127
1107	2	-0.10871540395400814
1107	3	-0.09547256152118067
1107	4	-0.043150042130641
1107	5	-0.07021840160873438
1107	6	-0.053307522450637634
1107	7	0.031053338523378216
1107	8	-0.01821744506886347
1107	9	0.019830142865281175
1107	10-11	0.002804599296062804
1107	12-13	0.029621909435128657
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2316	84-85	0.16318863407402162
2316	86-87	0.07232963201832021
2316	88-89	0.1517715883173274
2316	90-91	0.2854258013159843
2316	92-93	0.4274740058343589
2316	94-95	0.39819456301860257
2316	96-97	0.32698195534858954
2316	98-99	0.3360783872249158
2316	100	0.12892173794093154
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17178.0
1	1652.0
2	971.0
3	739.0
4	634.0
5	550.0
6	698.0
7	2509.0
8	5194.0
9	6407.0
10	8649.0
11	12602.0
12	18124.0
13	23173.0
14	27618.0
15	32266.0
16	38453.0
17	46926.0
18	57917.0
19	69371.0
20	81490.0
21	95414.0
22	107842.0
23	116310.0
24	119883.0
25	125140.0
26	134449.0
27	149609.0
28	172772.0
29	204002.0
30	248059.0
31	307190.0
32	393681.0
33	526232.0
34	764048.0
35	1248819.0
36	1402532.0
37	1355723.0
38	1315989.0
39	304317.0
40	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.446206833168475	17.113000377275036	11.730399848586394	23.710392940970095
2	16.546167215881376	20.08248232398073	39.87510550363354	23.496244956504356
3	19.209603651087352	26.755030748880447	29.208446607481942	24.826918992550258
4	12.863965234878114	16.008753493158288	36.67982240800089	34.447458863962716
5	13.194634774216112	36.23095347789241	33.81378756339032	16.76062418450115
6	30.300645716546644	34.93343637853398	19.8662583090959	14.899659595823476
7	28.08755976730219	31.69133823928852	22.266963452377425	17.954138541031863
8	27.0713091782741	33.39765939001126	20.987554992949097	18.543476438765534
9	28.085269357820096	15.507096833560421	20.28420948361464	36.12342432500484
10-11	25.265509697806266	25.297478035289412	28.591634577117652	20.845377689786666
12-13	25.672207157518397	23.41028892284319	28.427405093022323	22.490098826616098
14-15	23.306075057693374	24.670177139852516	26.666857424719044	25.35689037773507
16-17	22.689065834906387	27.072667167964514	27.28173262902403	22.956534368105068
18-19	22.38937529352016	26.262084838492555	29.175585067112042	22.17295480087524
20-21	23.26341424630238	25.827059254895442	29.03196211595503	21.87756438284715
22-23	22.977787738391346	25.627460893506242	28.79708542831782	22.59766593978459
24-25	22.639343240498665	25.682418337352374	28.926549655436084	22.751688766712874
26-27	22.419688068444184	25.736075654318935	28.989594055041962	22.85464222219492
28-29	22.497394901616477	25.999425389577937	29.033044930575436	22.470134778230154
30-31	23.201789973044658	25.783778728953425	28.933747778902646	22.08068351909927
32-33	22.75797836321428	25.941105339973586	28.90378733810128	22.397128958710848
34-35	22.837703291512277	26.14625327634635	28.75855160459786	22.257491827543515
36-37	22.932877177213175	26.158331310424394	28.47446621589797	22.434325296464458
38-39	22.897048006442365	26.367694633002493	28.348261263528187	22.38699609702696
40-41	22.925771324673253	26.033648063981108	28.59777150113809	22.442809110207545
42-43	22.765838424287153	26.12377535777583	28.265958964616594	22.844427253320426
44-45	22.556150724291918	26.340749391772462	28.39058829508641	22.71251158884921
46-47	22.564243735662153	26.095205081995203	28.19682377399932	23.14372740834332
48-49	22.658456898190398	26.244225209226222	28.179608464197088	22.917709428386292
50-51	22.5662194124455	26.552579053061326	28.093189239981463	22.788012294511713
52-53	22.584474042241183	26.650289913897325	27.79225306457562	22.972982979285877
54-55	22.432505110304852	26.48699892226664	28.09048443379083	22.990011533637674
56-57	22.628266706328755	26.60834049819092	27.687961248374744	23.075431547105584
58-59	22.509650865210233	26.625784855723722	27.95410585384378	22.910458425222266
60-61	22.906764647182822	26.674469879387118	27.925612755837488	22.493152717592572
62-63	22.685133806263735	26.612511133955742	27.975687048907048	22.726668010873475
64-65	22.55787375789363	26.369120803304718	27.893357032190767	23.179648406610884
66-67	22.54839286050043	26.44433312384566	27.746258922352414	23.261015093301495
68-69	22.40902396544371	26.508161108624996	27.89349590421386	23.189319021717438
70-71	22.411715740984683	26.289764066603816	27.99686581391519	23.301654378496313
72-73	22.397559010406443	26.699616131311423	27.567597330025777	23.335227528256357
74-75	22.429465401754996	26.713828056926488	27.539873776377394	23.316832764941122
76-77	22.334441643029727	26.413441537976677	27.755425990125016	23.49669082886858
78-79	22.326475792883965	26.4818385568963	27.73918095051005	23.452504699709692
80-81	22.430941605827915	26.661006881549433	27.40393376948516	23.50411774313749
82-83	22.612259591925405	26.656217464422866	27.42905609363292	23.30246685001881
84-85	22.625511242878318	26.772448723376762	27.15128061547707	23.450759418267854
86-87	22.398774107227695	26.700250486736554	27.45107514323714	23.44990026279861
88-89	22.34132814482167	26.42541103003478	27.61406382226546	23.61919700287809
90-91	22.83385797749688	26.387590534144294	27.501532068812125	23.2770194195467
92-93	22.669045640320714	26.444046198302946	27.498761279446693	23.388146881929647
94-95	22.557571617151904	26.242324252748084	27.511992899571027	23.68811123052899
96-97	22.61248383685754	26.2200273737988	27.53899022440117	23.62849856494249
98-99	22.67975366219794	26.167018273451642	27.358110000570885	23.79511806377954
100	22.733262282619066	26.096932954153377	27.471505819868792	23.69829894335877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10214.0
1	10374.5
2	13429.0
3	12794.0
4	6748.0
5	4568.0
6	6023.0
7	9997.0
8	15096.0
9	16948.0
10	14444.0
11	11854.0
12	11648.0
13	12328.0
14	13216.0
15	13834.0
16	14394.0
17	15225.0
18	16297.0
19	17441.0
20	18525.5
21	20376.0
22	23182.0
23	26601.5
24	30831.0
25	36437.5
26	44304.0
27	53512.5
28	63134.5
29	75176.0
30	90834.5
31	105219.5
32	120819.5
33	139640.0
34	158576.5
35	176127.0
36	199334.5
37	222317.5
38	234533.0
39	245852.0
40	253593.0
41	260683.5
42	269558.0
43	280521.5
44	295941.5
45	312155.0
46	327537.0
47	343098.0
48	363020.5
49	381935.0
50	399644.0
51	397227.0
52	374665.5
53	360173.0
54	350128.0
55	332198.0
56	303565.5
57	273852.0
58	248531.5
59	220078.5
60	185143.0
61	151184.0
62	123342.5
63	97302.5
64	73517.0
65	55579.0
66	41961.0
67	32686.5
68	25604.5
69	19594.0
70	15258.5
71	11961.5
72	9585.0
73	7587.5
74	5864.0
75	4276.0
76	2983.5
77	1994.5
78	1350.5
79	944.5
80	647.0
81	468.5
82	363.5
83	268.0
84	203.0
85	156.5
86	112.5
87	89.5
88	68.5
89	47.5
90	36.5
91	35.5
92	27.5
93	18.0
94	16.0
95	13.0
96	10.0
97	7.5
98	7.0
99	5.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1468750602376317
2	0.07078707432422805
3	0.31320425648534056
4	0.3120204560711571
5	0.31302616261772004
6	0.2596713351005832
7	0.2603732344612053
8	0.2594094323540825
9	0.2694350694901319
10-11	0.24730428741081686
12-13	0.2572984962172863
14-15	0.23173678815881113
16-17	0.2400338671679555
18-19	0.2540299499409566
20-21	0.25640278882425366
22-23	0.24702667049952606
24-25	0.2468642907966956
26-27	0.24487906797821973
28-29	0.25451185099451806
30-31	0.24018053270599593
32-33	0.2525213901211122
34-35	0.23607389764086387
36-37	0.24461192717678895
38-39	0.24677524386288535
40-41	0.26418653845025647
42-43	0.24952522270114338
44-45	0.23429819701958865
46-47	0.23732579276913757
48-49	0.24658667388540476
50-51	0.23995529634400525
52-53	0.25678516683414476
54-55	0.26764365470406665
56-57	0.26296607165156294
58-59	0.25927848098083206
60-61	0.2404738637820768
62-63	0.2688536453929002
64-65	0.26144703572185846
66-67	0.2668998509040045
68-69	0.2722531430424802
70-71	0.29234108369908923
72-73	0.3009995884984047
74-75	0.28145116749958726
76-77	0.2779102423668968
78-79	0.2757626398455905
80-81	0.26340083021075417
82-83	0.2738559878510649
84-85	0.2834625805927131
86-87	0.2751183590891986
88-89	0.2835149611420133
90-91	0.2779992893007071
92-93	0.25787468225958793
94-95	0.2793611835825111
96-97	0.2783659531458082
98-99	0.2638565409896655
100	0.28750635899868504
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9545528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.04720065642692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33587067445387	39.09551316999556
2	16.48473200091288	17.819072364415213
3	5.349685390898797	8.674065593119888
4	2.201118324083987	4.7585713492122155
5	1.085186622593809	2.9325649570498915
6	0.5812177668714478	1.88479159627089
7	0.3990773385466936	1.5098309095706177
8	0.26119468637312965	1.1293473299840828
9	0.19062202191686153	0.9272328001266983
>10	0.9929282994549661	9.727875691135386
>50	0.06938794290358868	2.587385868722773
>100	0.04465518893253306	4.596228509607115
>500	0.0028174445663976134	1.0507492025192817
>1k	0.001255247910309707	1.1153329283888225
>5k	1.3947198928472722E-4	0.5606666144042092
>10k+	1.1157759142778178E-4	1.6307711154773963
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39131	0.4099406549328649	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30532	0.31985658624646013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20734	0.21721166183787843	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17772	0.18618142443246724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.047610986003079E-5
2	0.0	0.0	0.0	1.047610986003079E-5	1.047610986003079E-5
3	0.0	0.0	0.0	2.095221972006158E-5	1.047610986003079E-5
4	0.0	0.0	0.0	4.190443944012316E-5	1.047610986003079E-5
5	0.0	0.0	0.0	4.190443944012316E-5	1.047610986003079E-5
6	0.0	0.0	0.0	4.190443944012316E-5	3.1428329580092375E-5
7	0.0	0.0	0.0	4.190443944012316E-5	3.1428329580092375E-5
8	0.0	0.0	0.0	4.190443944012316E-5	5.2380549300153957E-5
9	0.0	0.0	0.0	8.380887888024633E-5	7.333276902021554E-5
10-11	0.0	0.0	0.0	8.904693381026172E-5	7.333276902021554E-5
12-13	0.0	0.0	0.0	1.099991535303233E-4	7.333276902021554E-5
14-15	0.0	1.047610986003079E-5	0.0	1.257133183203695E-4	8.904693381026172E-5
16-17	0.0	2.095221972006158E-5	0.0	1.257133183203695E-4	9.428498874027712E-5
18-19	0.0	2.6190274650076978E-5	0.0	1.6237970283047726E-4	1.3618942818040028E-4
20-21	0.0	3.1428329580092375E-5	0.0	2.566646915707544E-4	1.5714164790046187E-4
22-23	0.0	3.1428329580092375E-5	0.0	6.547568662519244E-4	1.7285581269050807E-4
24-25	0.0	3.666638451010777E-5	0.0	0.001335704007153926	1.9380803241056963E-4
26-27	0.0	4.190443944012316E-5	0.0	0.002749978838258083	2.1476025213063122E-4
28-29	0.0	4.190443944012316E-5	0.0	0.006694234200559675	2.199983070606466E-4
30-31	0.0	4.190443944012316E-5	0.0	0.018390810859284054	2.199983070606466E-4
32-33	0.0	4.190443944012316E-5	0.0	0.0464405950095165	2.357124718506928E-4
34-35	5.238054930015395E-6	4.190443944012316E-5	0.0	0.09129929743016835	2.619027465007698E-4
36-37	1.047610986003079E-5	5.2380549300153957E-5	0.0	0.15938353541050845	2.619027465007698E-4
38-39	1.047610986003079E-5	6.809471409020015E-5	0.0	0.2617351287430093	2.6714080143078515E-4
40-41	1.047610986003079E-5	7.857082395023093E-5	0.0	0.38134611306991084	2.7237885636080056E-4
42-43	1.047610986003079E-5	8.380887888024633E-5	0.0	0.4947290500850241	2.8285496622083134E-4
44-45	1.047610986003079E-5	8.380887888024633E-5	0.0	0.630431339156933	2.9856913101087754E-4
46-47	1.047610986003079E-5	8.380887888024633E-5	0.0	0.7875834631672549	3.090452408709084E-4
48-49	1.047610986003079E-5	8.904693381026172E-5	0.0	0.9575792978659745	3.352355155209853E-4
50-51	1.047610986003079E-5	9.952304367029252E-5	0.0	1.137103154482392	3.352355155209853E-4
52-53	1.047610986003079E-5	1.0476109860030791E-4	0.0	1.318701280851096	4.0333022961118545E-4
54-55	1.047610986003079E-5	1.0476109860030791E-4	0.0	1.5226030451118053	4.871391084914318E-4
56-57	1.047610986003079E-5	1.0476109860030791E-4	0.0	1.7523284201774905	4.923771634214472E-4
58-59	1.047610986003079E-5	1.0476109860030791E-4	0.0	2.0044569561788514	4.976152183514626E-4
60-61	1.047610986003079E-5	1.257133183203695E-4	0.0	2.2654430430668686	5.185674380715241E-4
62-63	1.047610986003079E-5	1.257133183203695E-4	0.0	2.5322381328722727	5.447577127216011E-4
64-65	1.047610986003079E-5	1.257133183203695E-4	0.0	2.807330301686821	5.55233822581632E-4
66-67	1.047610986003079E-5	1.466655380404311E-4	0.0	3.11991646769042	5.814240972317089E-4
68-69	1.047610986003079E-5	1.466655380404311E-4	0.0	3.4771884803019804	6.023763169517706E-4
70-71	1.047610986003079E-5	1.466655380404311E-4	0.0	3.86510311425413	6.285665916018475E-4
72-73	1.047610986003079E-5	1.466655380404311E-4	0.0	4.2266022371942125	7.80470184572294E-4
74-75	1.047610986003079E-5	1.466655380404311E-4	0.0	4.600609835307172	7.857082395023093E-4
76-77	1.5714164790046184E-5	1.5714164790046187E-4	0.0	5.00825622218069	7.961843493623402E-4
78-79	2.095221972006158E-5	1.5714164790046187E-4	0.0	5.448645690421735	8.118985141523864E-4
80-81	2.095221972006158E-5	1.6761775776049265E-4	0.0	5.895430823732328	8.48564898662494E-4
82-83	2.095221972006158E-5	1.7809386762052343E-4	0.0	6.351581599257789	9.218976676827097E-4
84-85	2.095221972006158E-5	1.8856997748055424E-4	0.0	6.816993255899517	9.218976676827097E-4
86-87	2.095221972006158E-5	1.8856997748055424E-4	0.0	7.3042633157641985	9.323737775427404E-4
88	2.095221972006158E-5	1.8856997748055424E-4	0.0	7.687348463070875	9.323737775427404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	20170	0.0	59.77844	2
ACCTGGG	22330	0.0	53.11097	3
GTACCTG	23455	0.0	51.915836	1
CCTGGGG	18930	0.0	39.51841	4
TATAACG	930	0.0	30.338964	2
GGTATCA	28710	0.0	29.143787	1
GTATCAA	41540	0.0	28.883326	1
CTGGGGG	11165	0.0	28.340588	5
GTACCCG	1915	0.0	26.033968	1
CATGGGG	22575	0.0	25.118834	4
TATCACG	1015	0.0	24.55513	2
TCAACGC	48560	0.0	24.487818	4
CAACGCA	48985	0.0	24.264229	5
ATCAACG	49310	0.0	24.137094	3
GTACATG	41960	0.0	23.897636	1
TGGGGGG	16100	0.0	23.876213	6
TATCAAC	49665	0.0	23.860664	2
TACCGGG	1245	0.0	23.795982	2
AACGCAG	50525	0.0	23.764164	6
ATGGGGG	14210	0.0	23.624176	5
>>END_MODULE