FastQCFastQC Report
Mon 6 Jun 2016
ERR523059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545528
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355560.3724885621832548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297120.31126617616123486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207090.21694975909137765No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158550.1660987218307882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG212600.061.5434572
ACCTGGG235500.054.8940853
GTACCTG241450.054.746951
CCTGGGG189500.043.644484
CTGGGGG110050.034.886545
GTACCCG19900.031.9011961
GGTATCA291850.029.3088841
TATAACG11800.028.6765172
GTATCAA411600.027.9337841
ATAACGC12350.027.7767723
TAACGCA12500.027.0675134
TACCCGG22650.026.5593662
TGGGGGG147750.026.1755566
TCAACGC486400.023.4381394
CAACGCA494400.023.1064035
ATCAACG492300.023.0617873
TATCAAC499700.022.7605082
AACGCAG508650.022.6991586
TACCGGG13100.022.6019132
GTACCGG16100.022.4900861
Produced by FastQC (version 0.11.5)