FastQCFastQC Report
Mon 6 Jun 2016
ERR523058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94960.36173584445663437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77350.29465319680624125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.15633571036752605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.052.0509381
TACCTGG29950.044.0992552
GTATCAA81750.042.5395471
GTACCTG37450.036.8173071
ACCTGGG35600.036.1699373
TCAACGC100300.034.1994674
ATCAACG102050.033.5707973
CAACGCA102100.033.5044945
TATCAAC103100.033.462562
AACGCAG106800.032.3758436
GTACATG98150.029.2466221
ACGCAGA122250.028.245747
CGCAGAG125850.027.3615288
TACATGG101300.027.2828432
GTGGTAT23350.025.9979441
ACATGGG104950.025.7025723
CCTGGGG35550.024.9815644
TATCACG2451.2732926E-1124.940152
CATGGGG78700.024.5991384
GCAGAGT138300.024.5582529
Produced by FastQC (version 0.11.5)