FastQCFastQC Report
Mon 6 Jun 2016
ERR523058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86410.32916590479673313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.2842536722130798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.14955506795879808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.055.8846661
GTATCAA79200.042.593731
TACCTGG29250.042.129512
TATAACG3100.034.896122
TCAACGC97800.033.490064
GTACCTG37400.033.462761
ACCTGGG35700.033.0403063
ATCAACG99700.032.9002233
CAACGCA99650.032.7740025
ATAACGC3300.032.75313
TATCAAC101050.032.5816232
TAACGCA3450.031.327864
AACGCAG105850.030.986916
ACGCAGA119250.027.3867267
CGCAGAG124200.026.377938
GTACATG97000.026.0467931
TACATGG101100.024.3776072
CCTGGGG33600.024.3350334
ACATGGG102500.023.3821723
GCAGAGT138750.023.3069279
Produced by FastQC (version 0.11.5)