FastQCFastQC Report
Mon 6 Jun 2016
ERR523057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5140435
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169330.32940791975776373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137210.2669229355103216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82460.16041443963399984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86500.047.5002441
TACCTGG62400.044.8991472
GTATCAA151800.040.0579221
ACCTGGG73500.037.5373543
GTACCTG78300.036.3055341
TCAACGC186800.031.7776974
CAACGCA190050.031.2342745
ATCAACG190150.031.2190673
TATCAAC191750.031.0121292
AACGCAG197300.030.4114366
GTACATG168750.027.8345661
ACGCAGA222750.026.9157267
TACATGG176150.026.0194642
TATCACG4550.025.8288462
CGCAGAG232150.025.7026868
TATAACG4250.025.4398962
ACATGGG177150.025.1789683
CCTGGGG70550.024.9821724
CATGGGG127100.023.7772334
GCAGAGT252850.023.2445359
Produced by FastQC (version 0.11.5)