##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523057_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5140435
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.644221160271456	33.0	31.0	34.0	30.0	34.0
2	32.02224792259799	34.0	31.0	34.0	30.0	35.0
3	31.939532938360276	34.0	31.0	34.0	28.0	35.0
4	34.43311859793967	35.0	34.0	37.0	32.0	37.0
5	34.53238899042591	35.0	34.0	37.0	32.0	37.0
6	34.6445888334353	36.0	34.0	37.0	32.0	37.0
7	34.66235639590813	36.0	35.0	37.0	32.0	37.0
8	34.848423333822915	36.0	35.0	37.0	32.0	37.0
9	35.940995265964844	38.0	35.0	39.0	32.0	39.0
10-11	35.87555051274843	38.0	35.0	39.0	32.0	39.0
12-13	35.83304175619378	38.0	35.0	39.0	32.0	39.0
14-15	36.854128784820745	38.0	36.0	41.0	32.0	41.0
16-17	36.87845269904201	38.0	36.0	41.0	31.5	41.0
18-19	36.75441339108461	38.5	35.0	41.0	31.5	41.0
20-21	36.751872652800785	39.0	36.0	41.0	31.0	41.0
22-23	36.76758348272082	38.5	36.0	41.0	31.5	41.0
24-25	36.66951444770724	38.0	36.0	41.0	31.0	41.0
26-27	36.535794246984935	38.0	35.5	41.0	30.0	41.0
28-29	36.37423933966678	38.0	35.5	41.0	31.0	41.0
30-31	36.31582375810608	38.0	35.5	40.0	30.0	41.0
32-33	36.08834203720113	38.0	35.0	40.0	29.5	41.0
34-35	36.007150075820434	38.0	35.0	40.0	29.0	41.0
36-37	35.8196494071027	38.0	35.5	40.0	29.0	41.0
38-39	35.68679061207855	37.5	35.5	40.0	28.5	41.0
40-41	35.47543320750092	37.0	35.0	40.0	27.5	41.0
42-43	35.39688859016795	37.0	35.0	40.0	27.0	41.0
44-45	35.34334370534789	37.0	34.5	40.0	26.5	41.0
46-47	35.15013904465284	37.0	35.0	40.0	26.0	41.0
48-49	35.01479028525796	36.5	34.5	40.0	26.0	41.0
50-51	34.19273174351976	37.0	33.5	39.0	24.5	40.0
52-53	34.39394691305308	37.0	34.0	39.0	25.0	40.0
54-55	35.020584639237725	37.0	34.5	40.0	26.0	41.0
56-57	35.007952634358766	37.0	35.0	40.0	26.0	41.0
58-59	34.92438567942207	37.0	34.5	40.0	25.0	41.0
60-61	34.65388561473883	37.0	34.0	40.0	25.5	41.0
62-63	34.541305161917236	37.0	34.0	40.0	24.5	41.0
64-65	34.34069976568131	37.0	34.0	39.0	25.0	41.0
66-67	33.99391792717931	36.0	34.0	39.0	24.5	41.0
68-69	33.670577781841416	36.0	33.0	38.5	23.5	41.0
70-71	33.43892909841288	36.0	33.0	37.5	21.5	40.0
72-73	33.11611322777158	35.5	33.0	37.5	21.5	39.5
74-75	32.74793358927795	35.0	32.0	37.0	20.0	39.0
76-77	32.35929770534984	35.0	32.0	36.5	20.0	39.0
78-79	32.1220060170005	35.0	31.5	36.5	20.0	38.5
80-81	31.96201498511313	35.0	31.5	37.0	19.5	37.5
82-83	31.682547488685294	35.0	31.0	36.5	18.5	38.0
84-85	31.353598187702012	35.0	31.0	35.5	18.5	37.5
86-87	31.17521775880835	35.0	31.0	36.0	18.0	37.0
88-89	30.89316964420326	35.0	30.5	35.0	18.0	37.0
90-91	30.694127637057953	34.0	30.0	35.0	18.0	37.0
92-93	30.48586393953041	34.0	29.5	35.0	17.5	36.5
94-95	30.22874046651694	34.0	29.0	35.0	17.0	36.0
96-97	29.978675053764903	34.0	29.0	35.0	16.5	36.0
98-99	29.797975949506217	34.0	29.0	35.0	15.0	36.0
100	28.67665401858014	33.0	25.0	35.0	10.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17321057488926428
1101	2	0.10164838226503292
1101	3	-0.05895505758276087
1101	4	0.0311375352638521
1101	5	-0.0029778618187208394
1101	6	0.028609366200477382
1101	7	0.012699833937496408
1101	8	0.06662643643053201
1101	9	0.051793920997880605
1101	10-11	0.06501207639940532
1101	12-13	-0.046176846885067846
1101	14-15	-0.017673020909448667
1101	16-17	-0.07050739974798148
1101	18-19	-0.04091523412584763
1101	20-21	-0.14148105683132428
1101	22-23	-0.10683382216791415
1101	24-25	-0.15596129395042624
1101	26-27	-0.07937949322735705
1101	28-29	-0.08228206808291105
1101	30-31	-0.14281420917637178
1101	32-33	-0.14423546666220943
1101	34-35	-0.2120661724839934
1101	36-37	-0.3459512287400983
1101	38-39	-0.3998751312961346
1101	40-41	-0.34683215128797684
1101	42-43	-0.2785567609061559
1101	44-45	-0.41094753890620694
1101	46-47	-0.5091413588763984
1101	48-49	-0.41789926085329654
1101	50-51	-0.4786802348937229
1101	52-53	-0.38387191776218543
1101	54-55	-0.2935619630699833
1101	56-57	-0.28071654516737965
1101	58-59	-0.37547562452758854
1101	60-61	0.03849193219588187
1101	62-63	-0.354422865032312
1101	64-65	-0.4850417491791319
1101	66-67	-0.49214827539070427
1101	68-69	-0.461781141317708
1101	70-71	-0.4140660781984806
1101	72-73	-0.5301619838766811
1101	74-75	-0.6036539448617333
1101	76-77	-1.0271774402117586
1101	78-79	-1.5307795075889388
1101	80-81	-0.6229646113935168
1101	82-83	-0.7368211382945127
1101	84-85	-0.5755780858085267
1101	86-87	-1.0528528042239529
1101	88-89	-0.7637130700610584
1101	90-91	-0.8764925665236127
1101	92-93	-0.8307917165815049
1101	94-95	-1.462898991428311
1101	96-97	-1.011472326634685
1101	98-99	-0.918653631603469
1101	100	-0.9556611065520464
1102	1	0.12427142171454619
1102	2	0.1373897672997657
1102	3	0.04875234777971116
1102	4	0.057259530773613676
1102	5	0.13337983950200538
1102	6	0.21625542618014748
1102	7	0.10309021044562172
1102	8	0.09553657315958475
1102	9	0.17101185155709686
1102	10-11	0.21971212524487527
1102	12-13	0.11041674852594241
1102	14-15	0.19851097972610887
1102	16-17	-0.36214808871559967
1102	18-19	0.06654029024201691
1102	20-21	0.09438019937137199
1102	22-23	-0.0019874588595456544
1102	24-25	0.060800364481181646
1102	26-27	0.04621727951199972
1102	28-29	-0.008632134141400627
1102	30-31	-0.02400674284508142
1102	32-33	-0.062394081122342016
1102	34-35	-0.5583284529261974
1102	36-37	-0.5584034719963782
1102	38-39	-0.1510887292879417
1102	40-41	-0.3910588108132984
1102	42-43	-0.05875511852879356
1102	44-45	-0.4948325969827252
1102	46-47	-0.17564613849238953
1102	48-49	-0.22565417230308782
1102	50-51	-0.20548008953596764
1102	52-53	-0.11763833690532266
1102	54-55	-0.1059470533682898
1102	56-57	-0.15245642366139123
1102	58-59	-0.021572456429517217
1102	60-61	-0.05185791833636699
1102	62-63	-0.01344863128705498
1102	64-65	-0.12311084064874933
1102	66-67	-0.16114526858524414
1102	68-69	-0.7036672592690465
1102	70-71	-0.5004490135404538
1102	72-73	-0.25299224455781655
1102	74-75	-0.1631867565133689
1102	76-77	-0.1900669124433847
1102	78-79	-0.21801606004455465
1102	80-81	-0.32245887476750923
1102	82-83	-0.8486947211290712
1102	84-85	-0.4718710037354299
1102	86-87	-0.5461852288161708
1102	88-89	-0.3841722282198212
1102	90-91	-0.5087076635555796
1102	92-93	-0.4631983839961862
1102	94-95	-0.8552260656732784
1102	96-97	-0.7403685490626692
1102	98-99	-1.3369286195222116
1102	100	-0.8111310111740266
1103	1	0.09720019358689314
1103	2	0.06515192611517051
1103	3	-0.02776930037745373
1103	4	-0.030030789053888896
1103	5	0.058782176274164044
1103	6	0.138068664585262
1103	7	-0.06457232758400977
1103	8	0.004316224652022527
1103	9	0.13897006136246404
1103	10-11	0.05070074055847584
1103	12-13	-0.039413291059602784
1103	14-15	-0.08749288262124821
1103	16-17	-0.12171214759422355
1103	18-19	-0.1231319838029421
1103	20-21	-0.08204125748855517
1103	22-23	-0.10400909608063102
1103	24-25	-0.1190784607179296
1103	26-27	-0.16041149785132092
1103	28-29	-0.26285013345709274
1103	30-31	-0.2242158895969908
1103	32-33	-0.11389012126799258
1103	34-35	-0.28638112138271055
1103	36-37	-0.3300910908115
1103	38-39	-0.36419262065032143
1103	40-41	-0.28652229632024273
1103	42-43	-0.26182412609732353
1103	44-45	-0.24491369985329214
1103	46-47	-0.330284419689697
1103	48-49	-0.3271603990610501
1103	50-51	-0.36834979588163463
1103	52-53	-0.13151599018516436
1103	54-55	-0.2386453447246737
1103	56-57	-0.14728683262634945
1103	58-59	-0.09235774940857056
1103	60-61	-0.21824395606995495
1103	62-63	-0.1870136305154091
1103	64-65	-0.23964859160687269
1103	66-67	-0.24509591674286924
1103	68-69	-0.19885484971155165
1103	70-71	-0.3432454955801276
1103	72-73	-0.27491491256923695
1103	74-75	-0.31451182676046585
1103	76-77	-0.190756267724133
1103	78-79	-0.4794914470631717
1103	80-81	-0.87617776831787
1103	82-83	-0.4754569400546629
1103	84-85	-0.43727361276238597
1103	86-87	-0.5324832028274642
1103	88-89	-0.47189746744843575
1103	90-91	-0.49805053223103
1103	92-93	-0.4017499394964723
1103	94-95	-0.598775500492188
1103	96-97	-0.7203109029318142
1103	98-99	-0.5958469909551596
1103	100	-0.7968335042785633
1104	1	0.171111682950432
1104	2	0.054637417756680406
1104	3	0.08489365051292808
1104	4	0.009157028580879967
1104	5	0.10466366729303189
1104	6	0.10811405580238898
1104	7	0.06872046327622172
1104	8	-0.47648665207402274
1104	9	0.04614415071110045
1104	10-11	0.06297733463899391
1104	12-13	-0.044954905640452125
1104	14-15	0.01604959310147791
1104	16-17	0.023997662712361034
1104	18-19	0.11520920116345224
1104	20-21	0.013054098315613771
1104	22-23	0.025493019826107854
1104	24-25	-0.017852444298227965
1104	26-27	-0.013097228428073038
1104	28-29	0.04328017233466852
1104	30-31	0.019357480477573574
1104	32-33	-0.02618562899844079
1104	34-35	-0.1372938129817598
1104	36-37	-0.10945430092380093
1104	38-39	0.028492394108035057
1104	40-41	0.084169043866126
1104	42-43	0.0555693662186556
1104	44-45	-0.06447481832571356
1104	46-47	-0.10278977328623995
1104	48-49	-0.016329411035734154
1104	50-51	-0.01735217798333366
1104	52-53	-0.002484825704442528
1104	54-55	-0.038364486279455434
1104	56-57	0.0065313565968523335
1104	58-59	-0.04397224386723053
1104	60-61	0.029301280490280135
1104	62-63	0.06573440248813966
1104	64-65	0.029663887961831392
1104	66-67	0.0738298743328869
1104	68-69	0.01943181118228665
1104	70-71	-0.007566429982311718
1104	72-73	-0.007035033993020079
1104	74-75	-0.06670344208953338
1104	76-77	-0.07781358456833942
1104	78-79	-0.10881252570893452
1104	80-81	-0.08758154219268732
1104	82-83	-0.06335639256157677
1104	84-85	0.009227871095212947
1104	86-87	-0.055771289958606474
1104	88-89	-0.19187038976624748
1104	90-91	-0.13933958540846803
1104	92-93	-0.19626763448100704
1104	94-95	-0.33905471749176286
1104	96-97	-0.276545997440671
1104	98-99	-0.4038515762039623
1104	100	-0.34374984655145013
1105	1	0.12771282983075238
1105	2	0.13448187153207414
1105	3	0.12618583539586226
1105	4	0.10848109431965014
1105	5	0.12579875653185013
1105	6	0.11305112612898682
1105	7	0.06213403588124322
1105	8	0.08885415049429923
1105	9	0.12449527396277205
1105	10-11	0.1518274794322494
1105	12-13	0.09731608628242583
1105	14-15	0.08646935408801681
1105	16-17	0.004097615764948159
1105	18-19	-0.004410323381677017
1105	20-21	0.00158637969639841
1105	22-23	0.061229683585033
1105	24-25	0.02156370344202685
1105	26-27	0.04900408656425981
1105	28-29	0.07822407560130529
1105	30-31	0.09613342511990908
1105	32-33	0.037589097800157845
1105	34-35	0.117925639050533
1105	36-37	0.12658721112371296
1105	38-39	0.1017506916368589
1105	40-41	0.024071786803482098
1105	42-43	-0.05867704261427775
1105	44-45	-0.0344214253293913
1105	46-47	2.401940365004407E-4
1105	48-49	-0.015301836016718084
1105	50-51	-0.1315254542153923
1105	52-53	0.06990719137635892
1105	54-55	0.017132079432194303
1105	56-57	0.028096874575545883
1105	58-59	0.02835388414890616
1105	60-61	0.022245495190247766
1105	62-63	0.01825860054733397
1105	64-65	0.04516652739796001
1105	66-67	0.1640591574319643
1105	68-69	0.17500515063066047
1105	70-71	-0.022385426795160868
1105	72-73	0.14681523475380232
1105	74-75	0.011683782636723095
1105	76-77	0.01608030808477423
1105	78-79	0.04285554060001573
1105	80-81	-0.007454396827700549
1105	82-83	2.0695468037601472E-4
1105	84-85	0.04736954381752767
1105	86-87	-0.014677392037462766
1105	88-89	-0.055369397363051576
1105	90-91	-0.20136230609616135
1105	92-93	-0.15949851503161128
1105	94-95	-0.18886541716313232
1105	96-97	-0.10797837380086506
1105	98-99	-0.052599296620506664
1105	100	0.07690244356805565
1106	1	0.23088463569731132
1106	2	0.22421395578576409
1106	3	0.16928780925229958
1106	4	0.1317651716652648
1106	5	0.20072012628031644
1106	6	0.13931450275670443
1106	7	0.21248274065414563
1106	8	0.1599492906325608
1106	9	0.2055575051118126
1106	10-11	0.1589524994454976
1106	12-13	0.16328193514388545
1106	14-15	0.22414333656134744
1106	16-17	0.178861421491888
1106	18-19	0.12907212604266505
1106	20-21	0.16952253762312353
1106	22-23	0.17880347840806365
1106	24-25	0.11347387411804277
1106	26-27	0.1977174148945977
1106	28-29	0.2577906436497628
1106	30-31	0.27475550062468557
1106	32-33	0.21063931417088355
1106	34-35	0.21205157037795885
1106	36-37	0.18012191125126975
1106	38-39	0.17585178377216693
1106	40-41	0.19712078910036013
1106	42-43	0.274194139597725
1106	44-45	0.30652222874454793
1106	46-47	0.2172973245577836
1106	48-49	0.17761673680912082
1106	50-51	0.09086800511351356
1106	52-53	0.1704782423924982
1106	54-55	0.21297956747546465
1106	56-57	0.16902254518061
1106	58-59	0.16918075949463685
1106	60-61	0.22797821910909022
1106	62-63	0.24909309800043644
1106	64-65	0.20160787207580455
1106	66-67	0.2535145846076432
1106	68-69	0.29393373804815326
1106	70-71	0.26563426320625894
1106	72-73	0.1691179041227855
1106	74-75	0.23518517920104642
1106	76-77	0.24354171002138258
1106	78-79	0.18008706903552962
1106	80-81	0.2781616356244925
1106	82-83	0.22080624050143527
1106	84-85	0.23019540692918383
1106	86-87	0.22046737109293701
1106	88-89	0.10264337621141806
1106	90-91	0.1874955691076714
1106	92-93	0.09136852339458557
1106	94-95	0.09257236117278111
1106	96-97	0.18328139475113048
1106	98-99	0.17896292428478233
1106	100	0.1967772497904896
1107	1	0.1946030184726375
1107	2	0.031648435386244955
1107	3	-0.054213534020846055
1107	4	0.1721764799426566
1107	5	0.19378637333826987
1107	6	0.23393120106787535
1107	7	0.16847642432968968
1107	8	0.17385304621196696
1107	9	0.31977389581842175
1107	10-11	0.1687797363568606
1107	12-13	0.18560098350801013
1107	14-15	0.20581695170277925
1107	16-17	0.20256951749144037
1107	18-19	0.20559447453466362
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2314	96-97	0.27399103983053763
2314	98-99	0.3388141364479047
2314	100	0.35147424857464316
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2315	90-91	0.25997438093222414
2315	92-93	0.35149681375027697
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2315	96-97	0.3955070735580968
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2315	100	0.4627270932266967
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2316	2	-2.657106281180443
2316	3	-0.2593244149522924
2316	4	-0.19648890586340428
2316	5	-0.08868416377889332
2316	6	-0.3097467633933917
2316	7	-0.234434959473397
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2316	9	-3.966569906113577
2316	10-11	-0.28480052987818993
2316	12-13	-0.1935939551019814
2316	14-15	-0.33339941695444253
2316	16-17	-0.35271595448784865
2316	18-19	-0.4406515948500953
2316	20-21	-0.26629579234386114
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2316	24-25	-0.3259351383862139
2316	26-27	-0.2757319629224142
2316	28-29	-0.27736324069294227
2316	30-31	-0.689495282613855
2316	32-33	-0.4063808052231934
2316	34-35	-0.27389168381959195
2316	36-37	-0.32545539551609437
2316	38-39	-0.2288096593208664
2316	40-41	-0.373232665222865
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2316	52-53	-0.364303266386635
2316	54-55	-0.5280546432022106
2316	56-57	-0.7478463841607308
2316	58-59	-0.5757115451631805
2316	60-61	-0.4623201587156487
2316	62-63	-0.24706985555689442
2316	64-65	-0.31354271305257697
2316	66-67	-0.446068544206085
2316	68-69	-0.14344315266530572
2316	70-71	-0.41042038598104824
2316	72-73	-0.11936886034442296
2316	74-75	-0.1081404568774218
2316	76-77	-0.13275609892142626
2316	78-79	-0.25850416713234026
2316	80-81	-0.22674468043545204
2316	82-83	-0.4604793800192972
2316	84-85	-0.23398804470692625
2316	86-87	-0.08483461473453247
2316	88-89	0.034100181090586545
2316	90-91	-0.07457164581856546
2316	92-93	-0.02249073803142565
2316	94-95	0.17741590803882445
2316	96-97	0.1801799007555438
2316	98-99	-1.707850531886379
2316	100	-0.23483972838771194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	7419.0
1	509.0
2	332.0
3	202.0
4	162.0
5	201.0
6	337.0
7	1801.0
8	3210.0
9	3268.0
10	4288.0
11	7012.0
12	10671.0
13	13710.0
14	15513.0
15	17154.0
16	19868.0
17	23217.0
18	28219.0
19	33625.0
20	39467.0
21	46608.0
22	52691.0
23	57041.0
24	58601.0
25	60247.0
26	64298.0
27	71054.0
28	81756.0
29	95790.0
30	116144.0
31	145033.0
32	186298.0
33	255531.0
34	394754.0
35	724073.0
36	723358.0
37	775458.0
38	805370.0
39	195955.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.313088461330885	18.282907220355384	12.807373475882109	23.596630842431622
2	16.070035536145628	20.50937415654028	40.05608611303554	23.364504194278553
3	18.57923603812985	26.63507376983655	30.028759220010958	24.75693097202264
4	12.894055764433626	16.715331953665785	37.81048124922625	32.58013103267434
5	13.308034091777635	37.00596091362719	34.3658408374448	15.320164157150368
6	30.03329489665367	36.34864407400737	19.155558622173675	14.462502407165283
7	26.960429025082995	32.34521235844882	22.9867615897009	17.70759702676728
8	26.076136931553716	34.1514715846053	21.186214375988715	18.586177107852272
9	27.15575618127423	15.78048455295456	20.660281955458803	36.403477310312404
10-11	24.73668002432445	25.667772441644708	29.001255204029125	20.594292330001714
12-13	25.293738315689495	23.77017683015906	28.678408305395266	22.25767654875618
14-15	23.051328353194386	25.080330270051583	26.57400167910142	25.294339697652614
16-17	22.413275288507435	27.690652287079047	27.096398989899974	22.799673434513544
18-19	22.275417110458218	26.86133949179082	28.912862484992953	21.95038091275801
20-21	23.1187232914182	26.282360317729008	28.72045653591418	21.878459854938615
22-23	22.96049632073098	26.061682212750263	28.54541052829785	22.432410938220908
24-25	22.58444035307562	26.088388183997775	28.68229284570575	22.64487861722085
26-27	22.44348292633958	26.211388547906783	28.68900271383314	22.656125811920496
28-29	22.469447987387113	26.46222931817843	28.702893792839163	22.365428901595294
30-31	23.050438408632846	26.222431166906357	28.610043241998973	22.117087182461823
32-33	22.762356358249107	26.367440228462264	28.467811577110886	22.402391836177742
34-35	22.932317271172586	26.483931667045447	28.36891383066765	22.214837231114316
36-37	22.918267020142963	26.393079884772604	28.29421697469796	22.394436120386473
38-39	22.75170717243431	26.574527733790852	28.2346212171868	22.439143876588037
40-41	22.81686518752832	26.29853544558594	28.417095410896458	22.46750395598928
42-43	22.693518290362626	26.53403827439287	28.10065157857308	22.671791856671426
44-45	22.494960626422106	26.641777430233276	28.206478075265302	22.656783868079316
46-47	22.550582986972195	26.39512096383983	27.95848480592975	23.095811243258225
48-49	22.62760184170822	26.58712831528922	27.87853653591994	22.906733307082625
50-51	22.551698762387698	26.77955198385914	27.873202611193825	22.79554664255934
52-53	22.584233321973812	26.89191597177328	27.640542886353053	22.883307819899855
54-55	22.463843900035158	26.66093690895426	27.904995444881603	22.97022374612898
56-57	22.579007468464177	26.821343504705542	27.626328017785344	22.973321009044938
58-59	22.502121396002856	26.765812990595673	27.823182069634427	22.908883543767043
60-61	22.704496285235003	26.7418635257709	27.851034741412846	22.702605447581252
62-63	22.622858780389382	26.713404565985726	27.86136340154435	22.802373252080542
64-65	22.541504783438597	26.525932801231473	27.79940769697604	23.133154718353886
66-67	22.42673548511862	26.581692409903866	27.69529081228439	23.296281292693124
68-69	22.241792051828888	26.647235902095222	27.827157237423688	23.2838148086522
70-71	22.386005547829928	26.37311227799785	27.87407761600263	23.366804558169598
72-73	22.228308422271695	26.739697167238962	27.55088211658841	23.48111229390093
74-75	22.287008276754534	26.764580493750785	27.56127380850133	23.387137420993355
76-77	22.28752120645868	26.526257976831925	27.60469727509013	23.58152354161927
78-79	22.15771009921616	26.43670324976081	27.774965694205907	23.630620956817125
80-81	22.217902114950935	26.68645334308527	27.408691412202106	23.686953129761687
82-83	22.43449797096401	26.55731303812996	27.501924300228087	23.50626469067794
84-85	22.358982990321906	26.6693606872639	27.33513612979042	23.636520192623774
86-87	22.154470633087396	26.615499520765063	27.61484331503823	23.61518653110932
88-89	22.05232410464821	26.378435405623215	27.709827089519823	23.85941340020876
90-91	22.46920506150213	26.30368700787133	27.599179386622502	23.627928544004035
92-93	22.341118791858758	26.262755666623182	27.543611495733472	23.852514045784588
94-95	22.265042463902905	26.05489875027473	27.635460035528137	24.044598750294234
96-97	22.265090850437172	25.92701850906632	27.73268569772406	24.075204942772448
98-99	22.25471073081006	25.930887762583026	27.652696369597702	24.16170513700921
100	22.370757153676216	25.80981508581018	27.661472982564128	24.157954777949477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4418.0
1	4761.5
2	4350.5
3	3098.5
4	2009.5
5	1564.5
6	2043.0
7	3373.0
8	5399.0
9	6407.0
10	5668.0
11	4918.5
12	5001.0
13	5246.5
14	5602.5
15	5988.0
16	6346.5
17	6790.0
18	7248.0
19	7810.5
20	8552.0
21	9613.5
22	11158.5
23	13259.0
24	16012.5
25	19705.5
26	24394.0
27	29830.0
28	36035.0
29	43218.5
30	51744.0
31	60231.5
32	69204.0
33	79038.5
34	88413.5
35	98136.0
36	112010.0
37	124829.0
38	132773.0
39	140072.5
40	145483.5
41	150930.0
42	157392.5
43	165248.0
44	173637.0
45	180837.0
46	187285.5
47	193346.0
48	201308.0
49	208200.0
50	209084.5
51	205230.5
52	199217.5
53	192897.0
54	184568.0
55	172486.0
56	157506.5
57	140839.0
58	124842.0
59	108217.0
60	90648.5
61	74442.5
62	60530.5
63	47566.5
64	35420.5
65	26328.0
66	19596.0
67	14855.5
68	11571.0
69	8704.5
70	6733.5
71	5266.0
72	4081.0
73	3167.5
74	2415.5
75	1795.5
76	1303.5
77	909.5
78	616.0
79	439.5
80	327.0
81	245.0
82	181.5
83	144.0
84	109.5
85	95.0
86	72.0
87	50.0
88	48.5
89	32.5
90	19.0
91	18.0
92	17.5
93	13.5
94	10.5
95	10.5
96	12.0
97	8.0
98	4.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1532166052094813
2	0.1758606032368856
3	0.22653724830680672
4	0.21749132125977663
5	0.22743211420823334
6	0.1929603233967553
7	0.20747271388510896
8	0.19545038503550768
9	0.1996523640509023
10-11	0.190548076184214
12-13	0.19240589561000188
14-15	0.17739743815455306
16-17	0.18399221077593628
18-19	0.19316458626555924
20-21	0.18890424643050635
22-23	0.19850460126428987
24-25	0.18635582397209574
26-27	0.1991562970838071
28-29	0.19699694675645155
30-31	0.19240589561000188
32-33	0.20397106470561344
34-35	0.19276578733122782
36-37	0.19566437470758796
38-39	0.20920408486830394
40-41	0.2237748361763158
42-43	0.20891228077001264
44-45	0.1913651276594296
46-47	0.19125813282338944
48-49	0.20260931224692072
50-51	0.20615959544279813
52-53	0.21614902240763673
54-55	0.20409751314820632
56-57	0.21920323863641888
58-59	0.21685907904681218
60-61	0.20296920396814666
62-63	0.20941807454038422
64-65	0.21332824945748757
66-67	0.21015731158938886
68-69	0.21283218249039237
70-71	0.22181974871776414
72-73	0.23846230912364422
74-75	0.22709167609356018
76-77	0.25609700346371467
78-79	0.26748709010035143
80-81	0.2577116528075932
82-83	0.25769219920104036
84-85	0.25349022018564576
86-87	0.2425864737128278
88-89	0.24302417986026476
90-91	0.2349217527310432
92-93	0.23398797961651105
94-95	0.24643828781027288
96-97	0.25818826616813556
98-99	0.25585383338180523
100	0.257682472397764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5140435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.44590213595039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73590104290838	48.379984700684226
2	12.850782169866564	15.792558071600743
3	3.7961457509002003	6.997728009108529
4	1.5473197531665932	3.8030583250438963
5	0.8284475123236336	2.5452352383504775
6	0.5092693326381635	1.877550814447522
7	0.32407213612478	1.3939033332918138
8	0.233576574336074	1.1481858662323958
9	0.17882738291252848	0.9889388882704216
>10	0.9124568410115654	9.956235569847017
>50	0.056177532139036535	2.3669382581019613
>100	0.025140916880365346	2.7079153571812515
>500	0.0013221449372220367	0.5295888388841853
>1k	4.407148860529083E-4	0.5545270796862307
>5k	4.006498964116924E-5	0.20300245500965222
>10k+	8.012997928233848E-5	0.7546491942597477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16933	0.32940791975776373	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13721	0.2669229355103216	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8246	0.16041443963399984	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.945360655275283E-5
2	0.0	0.0	0.0	0.0	1.945360655275283E-5
3	0.0	0.0	0.0	0.0	1.945360655275283E-5
4	0.0	0.0	0.0	0.0	1.945360655275283E-5
5	0.0	0.0	0.0	0.0	1.945360655275283E-5
6	0.0	0.0	0.0	0.0	1.945360655275283E-5
7	0.0	0.0	0.0	0.0	1.945360655275283E-5
8	0.0	0.0	0.0	0.0	1.945360655275283E-5
9	0.0	0.0	0.0	0.0	5.836081965825849E-5
10-11	0.0	0.0	0.0	2.9180409829129246E-5	5.836081965825849E-5
12-13	0.0	0.0	0.0	4.863401638188208E-5	5.836081965825849E-5
14-15	0.0	0.0	0.0	6.80876229346349E-5	7.781442621101133E-5
16-17	0.0	0.0	0.0	7.781442621101132E-5	9.726803276376416E-5
18-19	0.0	0.0	0.0	1.1672163931651699E-4	9.726803276376416E-5
20-21	0.0	0.0	0.0	2.2371647535665757E-4	9.726803276376416E-5
22-23	0.0	0.0	0.0	5.738813933062085E-4	1.6535565569839906E-4
24-25	0.0	0.0	0.0	0.0013909328685218275	2.0426286880390472E-4
26-27	0.0	0.0	0.0	0.0028596801632546666	2.3344327863303397E-4
28-29	0.0	0.0	0.0	0.0075188189326389696	2.3344327863303397E-4
30-31	0.0	0.0	0.0	0.020825085814721905	2.3344327863303397E-4
32-33	0.0	9.726803276376415E-6	0.0	0.05196058310240281	2.723504917385396E-4
34-35	1.945360655275283E-5	1.945360655275283E-5	0.0	0.10247187251662554	2.723504917385396E-4
36-37	1.945360655275283E-5	1.945360655275283E-5	0.0	0.17807831438389943	2.820772950149161E-4
38-39	1.945360655275283E-5	1.945360655275283E-5	0.0	0.28570539263700445	3.1125770484404533E-4
40-41	1.945360655275283E-5	1.945360655275283E-5	0.0	0.4090996190011157	3.696185245023038E-4
42-43	1.945360655275283E-5	1.945360655275283E-5	0.0	0.5282432323334504	3.9879893433143304E-4
44-45	1.945360655275283E-5	1.945360655275283E-5	0.0	0.6783861677075967	4.0852573760780944E-4
46-47	1.945360655275283E-5	2.9180409829129246E-5	0.0	0.8551610904524617	4.0852573760780944E-4
48-49	1.945360655275283E-5	3.890721310550566E-5	0.0	1.0370231313108715	4.5715975398969154E-4
50-51	1.945360655275283E-5	3.890721310550566E-5	0.0	1.2305087020845513	4.6688655726606794E-4
52-53	1.945360655275283E-5	3.890721310550566E-5	0.0	1.4305112310534032	5.447009834770792E-4
54-55	1.945360655275283E-5	3.890721310550566E-5	0.0	1.6569706649339988	6.419690162408434E-4
56-57	1.945360655275283E-5	3.890721310550566E-5	0.0	1.9081848131529724	6.808762293463492E-4
58-59	1.945360655275283E-5	3.890721310550566E-5	0.0	2.1938512985768712	7.197834424518548E-4
60-61	1.945360655275283E-5	3.890721310550566E-5	0.0	2.4896239326049256	7.392370490046076E-4
62-63	1.945360655275283E-5	3.890721310550566E-5	0.0	2.7956583440895564	7.586906555573604E-4
64-65	1.945360655275283E-5	3.890721310550566E-5	0.0	3.098978977460079	7.586906555573604E-4
66-67	1.945360655275283E-5	5.836081965825849E-5	0.0	3.446906730656063	7.684174588337368E-4
68-69	1.945360655275283E-5	5.836081965825849E-5	0.0	3.840696361300162	7.781442621101133E-4
70-71	1.945360655275283E-5	5.836081965825849E-5	0.0	4.259396335135062	7.878710653864897E-4
72-73	1.945360655275283E-5	5.836081965825849E-5	0.0	4.661949815531176	8.948659014266303E-4
74-75	1.945360655275283E-5	5.836081965825849E-5	0.0	5.0708646252700404	8.948659014266303E-4
76-77	1.945360655275283E-5	5.836081965825849E-5	0.0	5.511965427050434	9.045927047030067E-4
78-79	1.945360655275283E-5	5.836081965825849E-5	0.0	6.009783218735379	9.143195079793831E-4
80-81	1.945360655275283E-5	7.781442621101132E-5	0.0	6.516608030254249	9.726803276376415E-4
82-83	1.945360655275283E-5	7.781442621101132E-5	9.726803276376415E-6	7.02898684644393	0.001099128770230535
84-85	1.945360655275283E-5	7.781442621101132E-5	1.945360655275283E-5	7.551111919516539	0.0011380359833360405
86-87	1.945360655275283E-5	7.781442621101132E-5	1.945360655275283E-5	8.084364455537322	0.0011866699997179226
88	1.945360655275283E-5	7.781442621101132E-5	1.945360655275283E-5	8.504319186994875	0.0011866699997179226
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8650	0.0	47.500244	1
TACCTGG	6240	0.0	44.899147	2
GTATCAA	15180	0.0	40.057922	1
ACCTGGG	7350	0.0	37.537354	3
GTACCTG	7830	0.0	36.305534	1
TCAACGC	18680	0.0	31.777697	4
CAACGCA	19005	0.0	31.234274	5
ATCAACG	19015	0.0	31.219067	3
TATCAAC	19175	0.0	31.012129	2
AACGCAG	19730	0.0	30.411436	6
GTACATG	16875	0.0	27.834566	1
ACGCAGA	22275	0.0	26.915726	7
TACATGG	17615	0.0	26.019464	2
TATCACG	455	0.0	25.828846	2
CGCAGAG	23215	0.0	25.702686	8
TATAACG	425	0.0	25.439896	2
ACATGGG	17715	0.0	25.178968	3
CCTGGGG	7055	0.0	24.982172	4
CATGGGG	12710	0.0	23.777233	4
GCAGAGT	25285	0.0	23.244535	9
>>END_MODULE