FastQCFastQC Report
Mon 6 Jun 2016
ERR523057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5140435
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164560.32012854943210056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139780.27192251239437903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83260.16197072815822006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.049.3260541
TACCTGG68900.047.579032
ACCTGGG76950.041.8355833
GTACCTG80850.041.375961
GTATCAA154750.040.7106861
TCAACGC189850.032.650174
ATCAACG193650.032.0346763
CAACGCA194300.031.9265775
TATCAAC199200.031.4733412
AACGCAG203350.030.7133986
CCTGGGG74950.030.0341364
ACGCAGA231200.027.054367
CGCAGAG238550.026.207718
GTACATG167900.025.9208661
TAACGCA6350.025.9027334
TATAACG5650.024.9732382
TATCACG5100.024.8997862
TACATGG173100.024.4538632
GCAGAGT264550.023.3652619
ACATGGG175550.023.2906883
Produced by FastQC (version 0.11.5)