FastQCFastQC Report
Mon 6 Jun 2016
ERR523056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941950
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT225860.4570260727040945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204990.41479577899412173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156540.3167575552160584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96250.19476117726808245No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63490.1284715547506551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4100.038.9827542
TACCTGG42050.031.8607712
GTACATG195850.030.1272431
TACATGG202000.028.9731312
ATAACGC6200.028.8134883
ACATGGG206800.027.7567793
ATGGGGG108400.027.234885
CATGGGG152000.027.1848624
GTACCTG53900.025.9936181
TAACGCA6700.025.9602434
TGGGGGG112550.023.3325126
ACCTGGG57050.023.320333
GGGTTAG20200.022.5766541
GAGTACT163000.021.01182412-13
GTATAAC12650.020.0697731
TATCACG5600.019.3071082
AGAGTAC251150.019.2748110-11
AGTACTT168800.018.6477412-13
GTACTTT184100.018.4512614-15
GAGTACA146350.018.3756431
Produced by FastQC (version 0.11.5)