FastQCFastQC Report
Mon 6 Jun 2016
ERR523056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941950
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT227790.4609314137132104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183380.37106810064852946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150520.30457612885601837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88740.17956474670929493No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61760.12497091229170673No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC49870.10091158348425217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43550.032.5938722
GTACATG192450.031.820341
TACATGG196650.030.665492
ATGGGGG102800.028.9832745
TATAACG3600.028.7235012
ACATGGG202400.028.6749953
CATGGGG145900.028.3129354
GTACCTG57450.025.461391
TGGGGGG102100.025.1775176
ACCTGGG57500.024.3554023
TATCACG5850.022.496732
ATAACGC4550.021.6897623
GGGTTAG20550.021.5143261
GAGTACT164450.021.18309812-13
TAACGCA4850.020.3483334
AGAGTAC247000.019.65151410-11
AGTACTT170900.018.85690912-13
GTTAAGA49950.018.8168014
TTAAGAG52150.018.6538015
GGTTAAG41700.018.594943
Produced by FastQC (version 0.11.5)