FastQCFastQC Report
Mon 6 Jun 2016
ERR523055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7409056
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214730.28982099743881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172210.23243176998527207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107510.14510620516297892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104300.050.6278841
GTATCAA194200.041.683141
TCAACGC231700.034.0546654
ATCAACG235800.033.5069273
CAACGCA237050.033.2252585
TACCTGG66750.032.6083342
TATCAAC245000.032.4087642
AACGCAG246150.032.104326
GTACATG229800.028.221521
ACGCAGA281450.028.0107757
TACATGG233950.027.1676792
CGCAGAG292300.026.8727748
GTACCTG83550.026.5874521
ACATGGG237400.025.9358983
ACCTGGG86850.024.7859953
GCAGAGT329350.023.463029
CATGGGG159650.023.460724
ATGGGGG98000.020.7632885
GAGTACT202450.020.4450512-13
AGAGTAC294950.020.298110-11
Produced by FastQC (version 0.11.5)