FastQCFastQC Report
Mon 6 Jun 2016
ERR523055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7409056
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199710.2695485092837738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164960.22264644780657616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102140.13785831825268968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109600.049.9662171
GTATCAA193450.038.3527951
TACCTGG71450.032.7808572
TCAACGC229800.031.5130944
ATCAACG233400.031.0073133
CAACGCA234150.030.907585
TATCAAC242850.029.9989832
AACGCAG243600.029.8048326
GTACATG231600.028.1958351
GTACCTG87550.027.3524131
TACATGG238600.026.7092722
ACCTGGG87750.026.2953323
ACGCAGA280250.025.9067237
ACATGGG241750.025.1931693
CGCAGAG290200.025.0090268
CATGGGG161650.023.5476594
GCAGAGT334250.021.6284779
ATGGGGG93750.021.1032455
GTGGTAT63950.019.7164291
GAGTACT205300.019.35849212-13
Produced by FastQC (version 0.11.5)