FastQCFastQC Report
Mon 6 Jun 2016
ERR523054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9879419
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108800.044.2027131
GTATCAA192850.040.4324231
TCAACGC225200.033.7671664
CAACGCA229700.033.0635455
ATCAACG235850.032.2446753
AACGCAG242800.031.4458716
TATCAAC247550.030.9519842
ACGCAGA285750.026.4888927
GTACATG405600.026.3479081
TACATGG420400.025.0578022
CGCAGAG307350.024.6256798
ACATGGG433800.023.7176443
CATGGGG309500.022.0946354
GCAGAGT351350.021.0596479
ATGGGGG172550.019.5559315
GTATAGG30650.018.8843271
CAGAGTA330300.017.76464810-11
AGAGTAC304700.017.71537410-11
GAGTACA319800.017.113081
AGTACAT307550.016.430722
Produced by FastQC (version 0.11.5)