FastQCFastQC Report
Mon 6 Jun 2016
ERR523054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9879419
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108550.048.454071
GTATCAA193000.037.928931
TCAACGC221900.031.7247164
CAACGCA227600.030.9093995
ATCAACG231100.030.523393
AACGCAG240600.029.297176
TATCAAC248650.028.768422
GTACATG408450.025.9617081
TACATGG428800.024.4911522
ACGCAGA289350.024.2474677
ACATGGG435550.023.5002173
CGCAGAG306700.022.789978
CATGGGG303500.021.6312184
GCAGAGT356500.019.210859
ATGGGGG169300.019.1805975
GAGTACA327900.017.4465851
CAGAGTA339650.017.03672810-11
AGTACAT313100.017.0035112
AGAGTAC311800.016.71918910-11
TATACCG6400.016.1541375
Produced by FastQC (version 0.11.5)