##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523053_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2213970 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.6755881967687 29.0 27.0 30.0 18.0 30.0 2 30.873365492757355 34.0 33.0 34.0 15.0 35.0 3 29.824098339182555 34.0 30.0 34.0 14.0 34.0 4 30.193951137549288 34.0 31.0 35.0 13.0 35.0 5 30.3184013333514 35.0 31.0 35.0 13.0 36.0 6 30.690953806962153 34.0 31.0 35.0 12.0 36.0 7 30.868270121094685 35.0 31.0 36.0 14.0 36.0 8 31.234265595288104 35.0 32.0 36.0 11.0 38.0 9 31.37338220481759 35.0 31.0 37.0 12.0 37.0 10-11 31.3996366256092 36.0 31.5 37.0 11.5 37.0 12-13 31.65325681919809 35.5 32.0 36.5 12.5 38.0 14-15 31.633850955523336 36.0 32.0 37.0 12.0 38.0 16-17 31.63207021775363 36.0 31.5 37.0 11.5 38.0 18-19 31.643933522134446 36.0 31.0 37.5 11.5 37.5 20-21 31.8828735258382 36.0 32.0 37.0 11.0 38.0 22-23 31.862813633427734 36.5 31.5 38.0 11.0 38.0 24-25 31.66078424730236 36.5 31.0 37.0 11.5 38.0 26-27 31.82233160340926 36.0 31.5 37.5 11.0 38.0 28-29 31.783126916805557 36.0 31.5 37.5 12.0 38.0 30-31 31.8222503014946 36.0 31.5 38.0 11.5 38.5 32-33 31.788126307041196 36.5 31.0 37.5 12.5 38.0 34-35 31.479340505968914 36.5 31.0 37.5 11.5 37.5 36-37 31.280171592207665 36.0 30.5 37.0 11.0 37.5 38-39 31.366976743135634 36.0 30.0 37.5 11.5 38.0 40-41 31.44092489961472 36.0 30.0 37.5 11.0 39.0 42-43 30.90716382787481 36.0 30.0 37.5 10.5 38.0 44-45 30.84407783303297 35.5 29.0 37.0 10.5 38.0 46-47 30.47175300478326 36.0 29.0 37.0 10.0 38.0 48-49 30.334908783768526 35.5 23.0 37.5 10.5 38.0 50-51 30.294502409698413 35.5 23.5 37.0 10.5 38.5 52-53 30.352622890102396 36.0 23.5 37.0 11.0 38.5 54-55 30.43208309055678 35.5 25.0 38.0 11.0 38.5 56-57 30.270714824500786 36.0 23.5 37.5 11.0 38.0 58-59 30.4529379350217 36.0 25.0 37.0 10.5 38.5 60-61 30.14876850183155 36.0 24.0 37.0 10.5 38.5 62-63 29.9651314155115 35.5 23.0 37.0 11.0 38.0 64-65 29.773861886114084 36.0 24.0 37.0 10.0 38.0 66-67 29.95104337457147 36.0 23.0 38.0 10.0 38.0 68-69 29.78660776794627 36.0 23.0 37.5 10.0 38.0 70-71 29.3060170192008 35.5 22.0 37.5 10.0 38.0 72-73 29.40737250278911 35.5 23.0 37.0 10.0 38.0 74-75 29.03073167206421 35.0 23.0 37.0 9.0 38.0 76-77 28.72645406215983 34.5 22.0 37.0 9.5 37.5 78-79 28.570818258603325 35.0 22.5 37.0 9.0 37.5 80-81 28.39433348238684 35.5 22.0 37.0 9.0 37.5 82-83 28.38585662859027 34.5 21.0 37.0 9.5 38.0 84-85 27.941183710709723 34.5 21.0 37.0 8.0 37.0 86-87 27.878667280947802 34.0 19.0 37.0 7.0 38.0 88-89 27.597565685171887 34.5 20.0 37.0 7.0 37.5 90-91 27.346761247894054 34.0 20.0 37.0 7.0 38.0 92-93 27.257701098027525 33.5 18.5 37.0 7.0 37.5 94-95 26.98886909036708 33.0 18.0 37.0 8.0 38.0 96-97 26.84118371070972 33.0 18.5 36.5 7.0 37.5 98-99 26.846904881276622 33.0 12.0 36.0 7.0 37.5 100 25.955001648622158 33.0 11.0 36.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01579439238182445 1101 2 0.151832908845563 1101 3 0.03267670497877617 1101 4 0.16145030806480065 1101 5 -0.010753958239579475 1101 6 -0.15930666374361735 1101 7 -0.12334714723315798 1101 8 -0.20101161941265389 1101 9 -0.11327339848071816 1101 10-11 -0.010702173254419023 1101 12-13 -0.09742325210763525 1101 14-15 -0.09860926499572287 1101 16-17 -0.22982305247704815 1101 18-19 -0.2817671943919997 1101 20-21 -0.04805626655246087 1101 22-23 -0.2046407710319933 1101 24-25 -0.23302731991674008 1101 26-27 -0.21770008719230916 1101 28-29 0.08977128962861869 1101 30-31 -0.3158003226071635 1101 32-33 0.06267303948575531 1101 34-35 -0.7633072445243627 1101 36-37 0.025524360298639692 1101 38-39 -0.2532411500895293 1101 40-41 -0.1534592684643279 1101 42-43 -0.2441087016411707 1101 44-45 -0.43570679483418573 1101 46-47 -0.40716438953415945 1101 48-49 -0.47611975341833457 1101 50-51 -0.43717108763266666 1101 52-53 -0.30266032851915625 1101 54-55 -0.4549862530511426 1101 56-57 -0.40597383357657435 1101 58-59 -0.9974868404945241 1101 60-61 -0.48868316368133335 1101 62-63 -0.6122796837292697 1101 64-65 -0.5247560400610922 1101 66-67 -0.5067679182919846 1101 68-69 -1.0248317886437128 1101 70-71 -0.9062916471002644 1101 72-73 -1.0204773975713728 1101 74-75 -1.0514461423790955 1101 76-77 -1.4227527006856242 1101 78-79 -1.1748686904992844 1101 80-81 -1.1318665714868281 1101 82-83 -1.857594685152936 1101 84-85 -1.9116421246304185 1101 86-87 -1.3700444247117858 1101 88-89 -1.8726412579599447 1101 90-91 -1.3062591715113143 1101 92-93 -1.397548244863355 1101 94-95 -1.8038907439753942 1101 96-97 -1.4444280419138167 1101 98-99 -1.8447915293283366 1101 100 -2.494458870057308 1102 1 0.2008637319772255 1102 2 0.30118567849365263 1102 3 0.14459944683202153 1102 4 0.13842042515463504 1102 5 0.14778346487407745 1102 6 0.10462499244255241 1102 7 0.0819320880897969 1102 8 0.19457707048284334 1102 9 -1.667074993015067 1102 10-11 0.193857433986782 1102 12-13 -0.031884013920887355 1102 14-15 0.041790920927713415 1102 16-17 -0.023981429982811164 1102 18-19 0.018285040815417375 1102 20-21 0.057542156666915645 1102 22-23 0.2391760479910765 1102 24-25 0.047648374385516945 1102 26-27 0.08179790168529522 1102 28-29 -0.04547953527281123 1102 30-31 -0.00568132357826201 1102 32-33 -0.07359360630466938 1102 34-35 -0.08039257347903828 1102 36-37 0.29049358345717025 1102 38-39 0.22416076615303382 1102 40-41 0.14130443078756372 1102 42-43 0.08435060954104401 1102 44-45 0.10103070384431945 1102 46-47 -0.34220738878966017 1102 48-49 -0.11432046240756577 1102 50-51 0.1179078383829193 1102 52-53 0.30699390652500114 1102 54-55 0.2208127649626448 1102 56-57 0.12516399287674318 1102 58-59 0.1603467901468001 1102 60-61 0.33828325099841194 1102 62-63 0.09153029698697068 1102 64-65 0.073565809109855 1102 66-67 0.13634617883516853 1102 68-69 -0.005628324258445616 1102 70-71 -0.1172643416028123 1102 72-73 -0.13028798137899145 1102 74-75 -0.16581414766774216 1102 76-77 -0.23987594588821182 1102 78-79 -0.08852101545669555 1102 80-81 -0.2862376680648673 1102 82-83 -0.22533534069363625 1102 84-85 -0.3638435642880502 1102 86-87 -0.2597402605710144 1102 88-89 -0.517054432651701 1102 90-91 -0.46325061537541856 1102 92-93 -0.5648464400945628 1102 94-95 -0.45512404800170003 1102 96-97 -0.8064620791065309 1102 98-99 -1.4825516292131162 1102 100 -0.5689582752182893 1103 1 0.12295956256997798 1103 2 0.12232224124793944 1103 3 -0.09418909134934239 1103 4 0.3383239307566832 1103 5 0.2624995754955677 1103 6 0.2884440041354033 1103 7 0.2060064654691196 1103 8 0.1841923321096175 1103 9 0.3481325154363155 1103 10-11 0.13123464952328945 1103 12-13 0.305344950343688 1103 14-15 0.025673171764619696 1103 16-17 0.007062512014829991 1103 18-19 0.0752211768625557 1103 20-21 0.2458360039899823 1103 22-23 0.2767929396669082 1103 24-25 0.258360162473938 1103 26-27 0.2765967799758293 1103 28-29 -0.38803833099499485 1103 30-31 -1.9604818143292846E-4 1103 32-33 0.2436741896974226 1103 34-35 0.22459200994853745 1103 36-37 0.15831488987086217 1103 38-39 -0.007817594317451437 1103 40-41 0.17346399447863092 1103 42-43 0.03717938592968295 1103 44-45 0.020726739991374643 1103 46-47 0.1508793097153891 1103 48-49 0.24034779531177008 1103 50-51 0.27700021476220016 1103 52-53 0.2938207096963623 1103 54-55 0.017268188190449507 1103 56-57 0.09186995923759156 1103 58-59 0.22054378257196916 1103 60-61 0.19030885342762716 1103 62-63 -0.007402891799745959 1103 64-65 0.0847361080735034 1103 66-67 0.052855343419860645 1103 68-69 0.050917739576043175 1103 70-71 -0.04446609938670676 1103 72-73 -0.17110214365423104 1103 74-75 -0.37602743191762045 1103 76-77 -0.9781352164130972 1103 78-79 -1.0989171277766943 1103 80-81 -0.12796042453761558 1103 82-83 -0.5451093046245781 1103 84-85 -0.45142555419578656 1103 86-87 -0.7276888035359335 1103 88-89 -0.6665722206378639 1103 90-91 -0.5672228958755774 1103 92-93 -0.3680630789004766 1103 94-95 -0.46824438305867133 1103 96-97 -1.1022086346421531 1103 98-99 -0.5886383805799404 1103 100 -0.7878112094365797 1104 1 0.16344261461002674 1104 2 0.4214266899956982 1104 3 0.2613988445660347 1104 4 0.3528322840837248 1104 5 0.6045486982904649 1104 6 0.45002094444608787 1104 7 0.5020097669132433 1104 8 0.2242469475267761 1104 9 0.6240502331576643 1104 10-11 0.4335686686430016 1104 12-13 0.18058992544040464 1104 14-15 0.3374391637821006 1104 16-17 0.40462342339989377 1104 18-19 0.28784822936535903 1104 20-21 0.35898018057580217 1104 22-23 0.22893397884478972 1104 24-25 0.5451675088777925 1104 26-27 0.41962403329347 1104 28-29 0.3856217307689498 1104 30-31 0.3200477009606111 1104 32-33 -0.3854988045932579 1104 34-35 0.24691744306882413 1104 36-37 0.26058929478642057 1104 38-39 0.31744684928474953 1104 40-41 0.441981629663875 1104 42-43 0.4421781513415297 1104 44-45 0.4569132094232309 1104 46-47 0.3053769014051433 1104 48-49 0.35214104755366904 1104 50-51 0.3130112302239034 1104 52-53 0.501392749641763 1104 54-55 0.41544952328962026 1104 56-57 0.46607513915507326 1104 58-59 0.47359550318207866 1104 60-61 0.4016055940285703 1104 62-63 0.266023202559051 1104 64-65 0.422564320743831 1104 66-67 0.23714482067106246 1104 68-69 0.34217305672274634 1104 70-71 0.24662626075733485 1104 72-73 0.20003588330122923 1104 74-75 -0.06443270663075396 1104 76-77 -0.09666949263795033 1104 78-79 -0.12343050147444146 1104 80-81 -0.15307565995976447 1104 82-83 -0.19925357099862495 1104 84-85 -0.44419069495178576 1104 86-87 -0.5088946378714709 1104 88-89 -0.2517721850679102 1104 90-91 -0.16384226112459999 1104 92-93 -0.3903769647364683 1104 94-95 -0.5133217433883317 1104 96-97 -0.5606689521298804 1104 98-99 -0.5127240515009 1104 100 -0.5236490588012863 1105 1 0.13195343906140167 1105 2 0.021928862061709964 1105 3 0.09843391555938652 1105 4 -0.2101703823950487 1105 5 0.15832038139685167 1105 6 -0.009107276720570212 1105 7 0.027953414449576997 1105 8 -0.08685225685232822 1105 9 0.0858388812678399 1105 10-11 -0.047338245376298715 1105 12-13 0.12344845466025234 1105 14-15 0.026839559441992122 1105 16-17 -0.04665590068005443 1105 18-19 0.007975810551176465 1105 20-21 -0.11236722224966655 1105 22-23 0.0659928248778563 1105 24-25 -0.02678030015503552 1105 26-27 -0.0536717340362145 1105 28-29 -0.12856267405859612 1105 30-31 0.14903891954162773 1105 32-33 -0.2079117938916788 1105 34-35 -0.10760330508412608 1105 36-37 0.044617698610224465 1105 38-39 -0.22572851475974431 1105 40-41 -0.26435286223434673 1105 42-43 -0.0355565239703175 1105 44-45 -0.19232628109063654 1105 46-47 0.1380901024527148 1105 48-49 0.08960333439693002 1105 50-51 -0.11346316265343859 1105 52-53 -0.18700066501805424 1105 54-55 -0.10935357075081953 1105 56-57 -0.07862236777265252 1105 58-59 0.10855559677792925 1105 60-61 0.21554191712357706 1105 62-63 0.09179063382378772 1105 64-65 0.12109951893364368 1105 66-67 -0.018404111494639608 1105 68-69 -0.007595169060593321 1105 70-71 -0.14939565637109453 1105 72-73 -0.21611311789854426 1105 74-75 -0.04077859541846607 1105 76-77 -0.16450919094566174 1105 78-79 -0.04691723640717527 1105 80-81 -0.042901477534744714 1105 82-83 -0.026379421577690465 1105 84-85 -0.4213776798034097 1105 86-87 -0.252469703560827 1105 88-89 -0.15349935524813318 1105 90-91 -1.0652327983562024 1105 92-93 -0.5861851658715764 1105 94-95 -0.904848448917825 1105 96-97 -0.6527828579128538 1105 98-99 -0.5413656257024755 1105 100 -1.2244304579018532 1106 1 0.29864619153288174 1106 2 0.19838945653348716 1106 3 0.041060293850843976 1106 4 0.07741574057973821 1106 5 0.3495377545853309 1106 6 0.17097800580993194 1106 7 0.11324061703689026 1106 8 0.09086364176258854 1106 9 0.07786299601933067 1106 10-11 0.1069024463980135 1106 12-13 0.014794989746864218 1106 14-15 -0.02508534996735179 1106 16-17 0.14769007014617586 1106 18-19 -0.04061811501699708 1106 20-21 0.20516866095161745 1106 22-23 0.1722473867966663 1106 24-25 0.13809522383516182 1106 26-27 0.00677176570331639 1106 28-29 0.15572739952461134 1106 30-31 0.23661724863009326 1106 32-33 0.05890436531936416 1106 34-35 -0.07170428242048388 1106 36-37 0.08993405075712246 1106 38-39 0.06677051737549533 1106 40-41 0.05189957813339774 1106 42-43 0.26407773676470114 1106 44-45 -0.019411904895672905 1106 46-47 0.14752335949657436 1106 48-49 0.10532893353336092 1106 50-51 0.1108491330623238 1106 52-53 0.14250127591447637 1106 54-55 0.14124620808969013 1106 56-57 0.017831222819811643 1106 58-59 -0.006380335140459437 1106 60-61 0.008049443957798275 1106 62-63 0.023443347593417485 1106 64-65 0.11552699639667452 1106 66-67 0.04319880847318913 1106 68-69 0.005361052021594759 1106 70-71 -0.05834225210985622 1106 72-73 0.14915649641451267 1106 74-75 0.08043714891148213 1106 76-77 -0.29857083145467556 1106 78-79 -0.15667702968017494 1106 80-81 -0.11697461561884026 1106 82-83 -0.2807111340516677 1106 84-85 -0.3106120079863359 1106 86-87 -0.20577158243958493 1106 88-89 -0.39683945814866917 1106 90-91 -0.37998242028900364 1106 92-93 -0.381000596237449 1106 94-95 -0.5591085723160703 1106 96-97 -0.6623488446284185 1106 98-99 -0.5906035556377738 1106 100 -0.38318937388482155 1107 1 0.023335221715719 1107 2 -0.07653983012065879 1107 3 0.09334347186062075 1107 4 -0.13310672661363654 1107 5 -0.07855379664260553 1107 6 0.003514672523287743 1107 7 0.13201621535048247 1107 8 0.10423285152807438 1107 9 0.06196536702772448 1107 10-11 0.04877589072500044 1107 12-13 -0.13398584872844665 1107 14-15 -0.04775831856016666 1107 16-17 -0.10417756139135648 1107 18-19 -0.20667542063459976 1107 20-21 -0.15228802326189594 1107 22-23 -0.09566381141244662 1107 24-25 -0.1132636341391553 1107 26-27 -0.12474125684009252 1107 28-29 -0.20454360582018793 1107 30-31 -0.2016163019172872 1107 32-33 0.004617917152064166 1107 34-35 -0.10994706092560236 1107 36-37 -0.4342529826439616 1107 38-39 -0.04679845295744656 1107 40-41 -0.2177205430988991 1107 42-43 -0.19850773513411113 1107 44-45 -0.2527494677096378 1107 46-47 -0.2450963753149935 1107 48-49 -0.2980929703426298 1107 50-51 -0.10269054674402156 1107 52-53 -0.2620560993247665 1107 54-55 -0.2748030642611212 1107 56-57 -0.34338276445713944 1107 58-59 -0.5029879277722635 1107 60-61 -0.1575118589309632 1107 62-63 -0.36644857819073096 1107 64-65 -0.3773681970732241 1107 66-67 -0.3525205314312174 1107 68-69 -0.1932370605314766 1107 70-71 -0.29803021531007445 1107 72-73 -0.31499834624468903 1107 74-75 -0.1734174802688031 1107 76-77 -0.25726954110604794 1107 78-79 -0.27452948205114325 1107 80-81 -0.40705866006793556 1107 82-83 -0.4267546883643796 1107 84-85 -0.3971528462309486 1107 86-87 -0.5026293186402881 1107 88-89 -0.4146317022149688 1107 90-91 -0.5771328969706957 1107 92-93 -0.6801281422680674 1107 94-95 -0.4965075900329232 1107 96-97 -0.6996626479229668 1107 98-99 -0.4420960542144421 1107 100 -0.8411941328744597 1108 1 0.03385359586805592 1108 2 0.35167568654136616 1108 3 0.07861758803556995 1108 4 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-0.5676239154663527 2316 74-75 -0.36335735971750793 2316 76-77 0.13481874111743863 2316 78-79 -0.08904199603816565 2316 80-81 -0.48472237915179406 2316 82-83 0.5322424374397592 2316 84-85 0.5227151367099907 2316 86-87 0.6398865181864934 2316 88-89 0.7863921591336407 2316 90-91 0.6235587371446982 2316 92-93 0.9950669194715367 2316 94-95 1.0910040705471644 2316 96-97 1.0828310290731018 2316 98-99 1.319397513836062 2316 100 1.2288550288121094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 109.0 1 68.0 2 55.0 3 54.0 4 61.0 5 73.0 6 95.0 7 242.0 8 358.0 9 438.0 10 465.0 11 489.0 12 891.0 13 3535.0 14 12947.0 15 31000.0 16 53380.0 17 73165.0 18 84952.0 19 86292.0 20 81730.0 21 74238.0 22 63369.0 23 48844.0 24 39127.0 25 34565.0 26 33101.0 27 34876.0 28 38424.0 29 44448.0 30 52898.0 31 65301.0 32 84429.0 33 113629.0 34 164109.0 35 274749.0 36 500195.0 37 108989.0 38 8280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.95055576762792 17.3867966507123 14.487363373320894 18.175284208338883 2 15.86186139501589 23.9633006626922 33.341136446624894 26.83370149566701 3 29.72468229964873 22.834642109593624 30.80515734700428 16.63551824375336 4 13.978929300246392 15.854166412315221 40.080723235332286 30.0861810521061 5 11.61747017915118 36.99747687132044 34.03008763124339 17.354965318284997 6 26.10988989132877 33.08883322959369 15.895173688190722 24.90610319088682 7 22.11176042259435 33.19085041825071 23.661279073814345 21.036110085340592 8 24.38961111748918 21.116440566577566 21.36657141746541 33.127376898467844 9 21.943239434455343 21.59757055523064 26.953720686167188 29.505469324146834 10-11 26.00846165472409 21.794194320746282 25.436926061169086 26.760417963360545 12-13 32.29186482877664 21.460182689461803 25.09537763579141 21.152574845970147 14-15 25.952814010289455 23.951069728348422 30.775324285059053 19.32079197630307 16-17 19.755074335002597 26.944218356047873 30.38472888749897 22.915978421450564 18-19 19.546757060962143 30.33633701085541 28.881244049909693 21.23566187827276 20-21 24.001067510551383 23.750844302772567 25.756855715915645 26.4912324707604 22-23 21.046987108915292 24.062973970168464 29.057885146238156 25.832153774678083 24-25 27.06429439757049 24.283415959038138 25.951129569457564 22.701160073933814 26-27 24.016859518175522 20.468772878708723 34.02978005708718 21.48458754602857 28-29 19.782313232594266 29.801847196723624 28.654867956503317 21.76097161417879 30-31 21.90838182006746 23.614905482218845 27.91611243303724 26.56060026467645 32-33 22.119723318479263 24.459374827729246 30.755605520978396 22.665296332813096 34-35 24.975809208247753 22.263367968206193 29.0128869199378 23.74793590360825 36-37 21.93690854585584 21.216692758986035 34.03382739309491 22.812571302063215 38-39 21.32769535775151 23.874588627783368 32.22573830130609 22.57197771315903 40-41 20.24401589901195 25.30213929054511 31.122385927210033 23.331458883232905 42-43 19.12538310336209 25.496616306361563 27.819620406205946 27.5583801840704 44-45 21.418096191827686 21.307919753584464 29.888076558951397 27.385907495636445 46-47 23.789556625839367 25.109512215189127 30.0890066462274 21.011924512744105 48-49 21.538792273283367 23.584221952765503 30.807334828908544 24.069650945042582 50-51 16.050920601510285 24.82613271417412 34.57391213147007 24.549034552845526 52-53 19.407814890736628 27.115103409776637 27.7469528525549 25.730128846931834 54-55 18.078836347595146 22.64262488915164 29.72975599349509 29.548782769758127 56-57 22.332463703538338 21.111283842013286 28.355617879275258 28.200634575173115 58-59 25.270897098412163 20.754815873110545 27.82583785845124 26.14844917002605 60-61 21.15762430104277 22.346142438295345 31.291394431112685 25.204838829549196 62-63 19.32115902873627 22.625027954667132 30.836042206464697 27.217770810131903 64-65 19.343274613365384 25.99329968870435 28.15375474963065 26.50967094829962 66-67 20.686700941766972 22.742348425764344 26.160783597837217 30.41016703463147 68-69 19.200115132957553 22.289139613848217 28.496581820876525 30.0141634323177 70-71 22.77666230544221 22.00971639539489 27.77227133338018 27.441349965782724 72-73 20.79195461764553 19.27864467432829 32.749034766480136 27.180365941546047 74-75 19.207394093926126 24.750185177736732 29.193049880713453 26.849370847623693 76-77 20.42459160848641 21.10978558033394 29.196276463544134 29.269346347635512 78-79 21.09568227874951 21.319475771592842 30.601066356679436 26.98377559297821 80-81 22.955850892376542 20.21295603385594 29.528850756170332 27.30234231759719 82-83 21.485311261392617 19.64905234782416 31.592855727870557 27.272780662912666 84-85 21.766699321078857 20.870551419857062 30.71975308837178 26.6429961706923 86-87 22.718434584146884 20.983096804855197 29.785511572833816 26.512957038164103 88-89 22.88417978790975 20.437087151798032 28.058453568101715 28.620279492190498 90-91 24.247640475835492 18.031734414038322 28.75332637561727 28.967298734508915 92-93 25.8008661138383 19.32231833659873 28.628342816243613 26.248472733319357 94-95 24.27488887021288 17.791000091271467 29.518434576884463 28.415676461631197 96-97 22.77247585216619 18.566767664810264 31.16658729592155 27.494169187101996 98-99 24.477399244137594 20.25391807958808 28.072704484185696 27.19597819208863 100 23.768358888740785 16.393355217527837 29.710482499045483 30.12780339468589 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4120.0 1 2476.0 2 816.0 3 733.0 4 590.0 5 562.0 6 839.5 7 1314.0 8 1767.0 9 1944.0 10 1608.0 11 1243.5 12 1216.0 13 1223.0 14 1199.0 15 1170.0 16 1195.0 17 1319.0 18 1460.5 19 1682.5 20 2028.0 21 2470.0 22 2977.5 23 3745.5 24 4855.5 25 6133.0 26 7759.0 27 9882.5 28 11916.0 29 13796.5 30 15947.0 31 17767.5 32 19368.5 33 21155.5 34 22735.5 35 23632.5 36 24832.0 37 26808.5 38 27209.0 39 26717.0 40 26584.5 41 26601.5 42 26978.0 43 28140.5 44 30257.5 45 35763.0 46 65480.5 47 142195.0 48 273812.0 49 319997.0 50 224965.0 51 137099.5 52 93659.0 53 60812.0 54 37954.5 55 29361.5 56 26522.0 57 25333.0 58 24430.5 59 23699.5 60 23280.5 61 22508.5 62 21346.0 63 20095.0 64 18864.5 65 17640.5 66 16223.5 67 14757.0 68 13235.0 69 11749.5 70 10429.5 71 9055.5 72 7748.0 73 6477.5 74 5242.5 75 4284.0 76 3469.5 77 2684.5 78 2045.5 79 1525.0 80 1118.0 81 801.5 82 578.0 83 398.5 84 251.5 85 157.0 86 86.0 87 47.5 88 31.0 89 16.5 90 10.5 91 8.0 92 3.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016486221583851633 2 0.053207586371992396 3 0.08943210612609927 4 0.11124811989322349 5 0.10975758479112184 6 0.07683030935378528 7 0.06960347249511059 8 0.06901629200034327 9 0.06540287357100594 10-11 0.036563277731857254 12-13 0.04178015058921304 14-15 0.04629692362588472 16-17 0.05496912785629435 18-19 0.05842445922934819 20-21 0.06077318120841746 22-23 0.05772435940866407 24-25 0.053297921832725824 26-27 0.0548336246651942 28-29 0.052281647899474704 30-31 0.04875856493087079 32-33 0.039928273644177656 34-35 0.03769247099102517 36-37 0.030488217997533842 38-39 0.029178353816899052 40-41 0.03958951566642727 42-43 0.06535770584063921 44-45 0.07269746202523068 46-47 0.08527667493236132 48-49 0.09539424653450589 50-51 0.08753506145069717 52-53 0.07475259375691631 54-55 0.0672095827856746 56-57 0.05198805765209104 58-59 0.034688816921638506 60-61 0.026355370668979253 62-63 0.02551976765719499 64-65 0.028907347434698753 66-67 0.040470286408578256 68-69 0.03958951566642727 70-71 0.04094454757742878 72-73 0.03500499103420552 74-75 0.05560147608142838 76-77 0.04607108497405114 78-79 0.024638996915044016 80-81 0.018722024237004115 82-83 0.018993030619204414 84-85 0.017366992326002608 86-87 0.019151117675487925 88-89 0.017344408460819253 90-91 0.027100638220030084 92-93 0.03911525449757675 94-95 0.03577284245043971 96-97 0.03288210770696983 98-99 0.03326603341508693 100 0.03636002294520702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2213970.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.24615251244384 #Duplication Level Percentage of deduplicated Percentage of total 1 91.23842083855111 34.895185583857604 2 3.7744101535412633 2.8871333275371147 3 1.4178419904991362 1.6268100302153075 4 0.766493609655912 1.1726172597885443 5 0.4783369413484557 0.914727380557447 6 0.3564572517561459 0.8179871048899288 7 0.2325845058046296 0.622682373672467 8 0.19592702229773912 0.5994763820886653 9 0.15512104126833393 0.5339504702014015 >10 1.130423297443816 8.578038387534882 >50 0.14076682933090257 3.729299146473395 >100 0.08997740852600597 6.959597452882173 >500 0.011679142449786553 3.198381745047881 >1k 0.008461419529947401 6.910083454364894 >5k 9.533993836560451E-4 2.9878941234003187 >10k+ 0.0021451486132261013 23.56613577748794 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 93678 4.231222645293296 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 62437 2.820137580906697 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40561 1.8320483114044002 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 29343 1.3253567121505712 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 29099 1.3143357859410922 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 28104 1.269393894226209 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 26641 1.2033135046997023 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 26510 1.1973965320216624 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 23658 1.0685781650157862 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22432 1.0132025275861913 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 22422 1.0127508502825242 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 20187 0.9118009729129122 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 19569 0.8838873155462812 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 16559 0.7479324471424635 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 15976 0.7215996603386676 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 14338 0.6476149179979855 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 13654 0.6167201904271512 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 11862 0.5357796176099947 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 9905 0.44738636928232994 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9652 0.43595893349955056 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9341 0.4219117693555017 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 9037 0.40818077932401975 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8482 0.38311268897049194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7981 0.3604836560567668 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5970 0.2696513502892993 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 5185 0.2341946819514266 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4720 0.2131916873309033 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4446 0.20081572921042293 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 4382 0.19792499446695302 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 4277 0.19318238277844776 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4250 0.1919628540585464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4235 0.19128533810304568 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC 4228 0.19096916399047864 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3856 0.17416676829405997 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3808 0.17199871723645757 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3726 0.16829496334638683 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3324 0.15013753573896665 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT 3313 0.14964069070493277 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3051 0.13780674534885295 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT 3000 0.1355031911001504 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2847 0.12859252835404272 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2697 0.12181736879903521 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 2654 0.11987515639326639 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 2446 0.11048026847698929 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2319 0.10474396672041626 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACCTGTC 2282 0.10307276069684773 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 2276 0.10280175431464744 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2268 0.1024404124717137 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTCTGTCTCTTATACA 2229 0.10067887098741177 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 4.51677303667168E-5 0.0 1.3550319110015041E-4 0.0 2 0.0 4.51677303667168E-5 0.0 1.3550319110015041E-4 0.0 3 0.0 4.51677303667168E-5 0.0 1.806709214668672E-4 0.0 4 0.0 4.51677303667168E-5 0.0 1.806709214668672E-4 0.0 5 0.0 4.51677303667168E-5 0.0 3.161741125670176E-4 0.0 6 0.0 4.51677303667168E-5 0.0 3.161741125670176E-4 0.0 7 0.0 4.51677303667168E-5 0.0 3.613418429337344E-4 0.0 8 0.0 4.51677303667168E-5 0.0 3.613418429337344E-4 0.0 9 0.0 4.51677303667168E-5 0.0 5.871804947673184E-4 0.0 10-11 0.0 4.51677303667168E-5 0.0 9.259384725176945E-4 0.0 12-13 0.0 4.51677303667168E-5 0.0 0.001174360989534637 4.51677303667168E-5 14-15 0.0 4.51677303667168E-5 0.0 0.0016260382932018049 9.03354607334336E-5 16-17 0.0 4.51677303667168E-5 0.0 0.0026197283612695746 1.3550319110015041E-4 18-19 0.0 4.51677303667168E-5 0.0 0.004765195553688623 1.3550319110015041E-4 20-21 0.0 4.51677303667168E-5 0.0 0.019851217496172035 1.3550319110015041E-4 22-23 0.0 4.51677303667168E-5 0.0 0.07538494198205034 1.3550319110015041E-4 24-25 0.0 4.51677303667168E-5 0.0 0.1584709819916259 1.3550319110015041E-4 26-27 0.0 4.51677303667168E-5 0.0 0.3041369124242876 1.3550319110015041E-4 28-29 0.0 4.51677303667168E-5 0.0 0.7064007190702675 1.3550319110015041E-4 30-31 0.0 4.51677303667168E-5 0.0 1.4373500995948454 1.3550319110015041E-4 32-33 0.0 4.51677303667168E-5 0.0 2.8835530743415676 1.806709214668672E-4 34-35 0.0 4.51677303667168E-5 0.0 4.737846492951576 1.806709214668672E-4 36-37 0.0 4.51677303667168E-5 2.25838651833584E-5 6.668405624285786 1.806709214668672E-4 38-39 0.0 4.51677303667168E-5 4.51677303667168E-5 8.790295261453409 2.25838651833584E-4 40-41 0.0 4.51677303667168E-5 4.51677303667168E-5 10.676973942736351 2.25838651833584E-4 42-43 0.0 4.51677303667168E-5 4.51677303667168E-5 12.506402525779482 2.7100638220030083E-4 44-45 0.0 6.775159555007521E-5 6.775159555007521E-5 14.447530906019503 3.161741125670176E-4 46-47 0.0 9.03354607334336E-5 9.03354607334336E-5 16.608964891123186 3.161741125670176E-4 48-49 0.0 9.03354607334336E-5 9.03354607334336E-5 18.049905825282185 3.613418429337344E-4 50-51 0.0 9.03354607334336E-5 9.03354607334336E-5 19.38788691807025 3.613418429337344E-4 52-53 0.0 9.03354607334336E-5 9.03354607334336E-5 20.587767675262086 3.613418429337344E-4 54-55 0.0 1.1291932591679202E-4 9.03354607334336E-5 21.91906394395588 3.613418429337344E-4 56-57 0.0 1.3550319110015041E-4 9.03354607334336E-5 23.721820982217466 4.065095733004512E-4 58-59 0.0 1.3550319110015041E-4 9.03354607334336E-5 24.91506208304539 4.065095733004512E-4 60-61 0.0 1.3550319110015041E-4 9.03354607334336E-5 26.09771135110232 4.065095733004512E-4 62-63 0.0 1.3550319110015041E-4 1.3550319110015041E-4 27.14433799915988 4.065095733004512E-4 64-65 0.0 1.3550319110015041E-4 1.3550319110015041E-4 28.20541832093479 4.51677303667168E-4 66-67 0.0 1.3550319110015041E-4 1.3550319110015041E-4 29.409093167477426 4.51677303667168E-4 68-69 0.0 1.3550319110015041E-4 1.3550319110015041E-4 30.73808588192252 4.51677303667168E-4 70-71 0.0 1.3550319110015041E-4 1.3550319110015041E-4 31.937537545675866 4.51677303667168E-4 72-73 0.0 1.3550319110015041E-4 1.3550319110015041E-4 32.842247184921206 4.51677303667168E-4 74-75 0.0 1.3550319110015041E-4 1.3550319110015041E-4 33.97098876678545 4.51677303667168E-4 76-77 0.0 1.806709214668672E-4 1.3550319110015041E-4 34.840964421378786 4.51677303667168E-4 78-79 0.0 1.806709214668672E-4 1.3550319110015041E-4 36.22510693460164 4.51677303667168E-4 80-81 0.0 1.806709214668672E-4 1.3550319110015041E-4 37.39154098745692 4.51677303667168E-4 82-83 0.0 2.032547866502256E-4 1.3550319110015041E-4 38.24758239723212 4.51677303667168E-4 84-85 0.0 2.25838651833584E-4 1.3550319110015041E-4 39.17959592948414 4.51677303667168E-4 86-87 0.0 2.7100638220030083E-4 1.3550319110015041E-4 40.00499103420552 4.968450340338849E-4 88 0.0 2.7100638220030083E-4 1.3550319110015041E-4 40.732394747896315 4.968450340338849E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 1170 0.0 50.2344 1 ACCCATG 1435 0.0 41.285328 3 TACCCAT 1645 0.0 35.728218 2 ATCATTA 2190 0.0 27.677584 94 GTATATG 490 0.0 24.949068 1 GTATCAT 3990 0.0 24.612514 94 CATTAAA 1125 0.0 23.389347 94 CGTATCA 4625 0.0 23.36677 94 TCATTAA 1535 0.0 23.264183 94 CATATAG 195 4.6596142E-7 21.701262 3 GAGTACT 8460 0.0 20.91466 12-13 TATCATT 3040 0.0 20.557043 94 GTACTTT 9000 0.0 19.790995 14-15 TATACCG 120 0.0050521907 19.592302 5 TATATGG 555 0.0 19.485075 2 CCGTATC 6110 0.0 19.456373 94 TACCGTG 145 6.598267E-4 19.450146 7 AGTACTT 8470 0.0 19.225428 12-13 AGATAGT 125 0.0064077685 18.80266 6 TTATGTA 1470 0.0 18.551872 3 >>END_MODULE