FastQCFastQC Report
Mon 6 Jun 2016
ERR523053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213970
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT2105649.510697976937358No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1143905.1667366766487355RNA PCR Primer, Index 26 (100% over 22bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT945874.2722801121966425No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT765503.457589759572171RNA PCR Primer, Index 26 (100% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT615632.7806609845661865No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT404761.8282090543232292No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT308421.3930631399702795RNA PCR Primer, Index 26 (100% over 27bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG292151.3195752426636314No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC288231.3018694923598784No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC285851.2911195725325997No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA261141.1795101107964425No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT256071.1566100715005172No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC238891.0790119107304976No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT229931.0385416243219194No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC226991.0252623115941046No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA207390.9367335600753398No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG202940.9166339200621508RNA PCR Primer, Index 26 (96% over 27bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA191930.8669042489283956No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC158200.7145534944014599No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC157600.7118434305794569No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT143080.6462598860869839No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG142630.6442273382204817No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC131580.5943169961652597RNA PCR Primer, Index 26 (95% over 22bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC115210.5203774215549443No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT99380.4488769043844316RNA PCR Primer, Index 26 (100% over 21bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC97170.43889483597338713No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG95290.43040330266444443No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG88370.39914723325067636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.38239000528462447No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT82050.3706012276589114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79550.3593092950672322No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC69660.31463840973454926RNA PCR Primer, Index 26 (96% over 28bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT55970.252803786862514No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG51580.2329751532315253No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG49310.22272207843828054No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC43150.194898756532383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42560.19223386044074672No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA42420.1916015122156127No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC42180.19051748668681148No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG40710.18387783032290408RNA PCR Primer, Index 26 (100% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.1838326625925374No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT40180.18148394061346812No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA39820.1798579023202663No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA39340.1776898512626639No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC38510.1739409296422264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.17195354950609087No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA33290.15036337439080025No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT32740.1478791492206308No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31820.14372371802689288No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC31510.14232351838552465RNA PCR Primer, Index 26 (95% over 22bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT29770.13446433330171592No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29500.13324480458181456No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG28380.1281860187807423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC28020.1265599804875405No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC24810.1120611390398244No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC24510.1107061071288229No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT24360.11002859117332214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.10975758479112184No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT23460.1059634954403176No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTC22150.10004652276227773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT3150.065.6871261
GTACCCA8250.058.141251
CTTTACA7700.057.408381
CTATACA6850.054.234381
GCGTCAG2100.053.744011
CGTCAGA2700.050.483182
ACCCATG10200.045.6191833
GAGATGT7450.045.447961
GGAGATG4700.044.001482
GTCAGAT3150.043.2712973
TACCCAT12550.037.0769462
TCGCTCA550.002716499134.18451394
GTCCCCG550.002716499134.18451394
TTACACA13250.032.989793
GATGTGT10400.032.5395553
GTGTATA10750.031.4779976
GGCACGT300.00415719931.3411192-93
GATCGCG600.00416020231.33580294
CCGTGCT600.00416020231.33580294
AGATGTG10650.031.3343872
Produced by FastQC (version 0.11.5)