FastQCFastQC Report
Mon 6 Jun 2016
ERR523052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8954097
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293500.3277829132295529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238740.266626550952039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130010.1451961040850909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151600.052.8223151
GTATCAA279500.042.1343351
TCAACGC340200.034.1453254
ATCAACG345600.033.6140633
CAACGCA346400.033.4659775
TATCAAC356050.032.7505042
AACGCAG359350.032.3979956
ACGCAGA405300.028.5626537
CGCAGAG421100.027.389178
GTACATG333600.025.301921
GCAGAGT467450.024.2999049
TACATGG345650.024.0543422
ACATGGG347500.023.1658843
GAGTACT267850.021.60038212-13
CATGGGG230150.021.5610584
CAGAGTA456050.020.79187610-11
AGAGTAC424250.020.53441610-11
GTACTTT287050.019.91899914-15
GTGGTAT83000.019.8969351
TGGTATC81450.018.9271372
Produced by FastQC (version 0.11.5)