FastQCFastQC Report
Mon 6 Jun 2016
ERR523052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8954097
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266370.2974839338908211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229200.2559722102630784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127780.14270562402886633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154400.053.4455681
GTATCAA276500.040.632041
TCAACGC335850.032.8261154
ATCAACG341150.032.344593
CAACGCA343800.032.108055
TATCAAC353500.031.3573472
AACGCAG357650.030.8904296
ACGCAGA412650.026.7727577
CGCAGAG427300.025.8535258
GTACATG342750.025.4347441
TACATGG354100.024.0525822
ACATGGG352350.023.5406993
GCAGAGT474400.023.0191529
GTGGTAT80950.022.5498851
CATGGGG228600.021.6876774
TGGTATC79750.021.4654452
GAGTACT272950.021.11546512-13
CAGAGTA461150.020.46979710-11
GTACTTT286150.019.93249514-15
AGAGTAC432600.019.7125510-11
Produced by FastQC (version 0.11.5)