##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523052_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8954097
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68831150701182	34.0	31.0	34.0	30.0	35.0
2	32.74914645217714	34.0	31.0	34.0	31.0	34.0
3	33.023815578499985	34.0	31.0	34.0	31.0	36.0
4	35.80719697363118	37.0	35.0	37.0	33.0	37.0
5	35.85966546933767	37.0	35.0	37.0	35.0	37.0
6	35.934325482513756	37.0	35.0	37.0	35.0	37.0
7	35.76035260730367	37.0	35.0	37.0	35.0	37.0
8	35.80909565755207	37.0	36.0	37.0	34.0	37.0
9	37.0315345031442	39.0	36.0	39.0	34.0	39.0
10-11	37.15538183247289	39.0	36.0	39.0	34.0	39.0
12-13	37.0257062772494	38.0	36.5	39.0	34.0	39.0
14-15	38.12383504444948	39.0	36.5	41.0	34.5	41.0
16-17	38.14514115717084	39.0	37.0	41.0	34.5	41.0
18-19	38.15485179577572	39.0	37.0	41.0	34.0	41.0
20-21	38.04760094736521	39.0	36.5	41.0	34.0	41.0
22-23	37.96563344131742	39.0	36.5	41.0	34.5	41.0
24-25	37.83617220139563	39.0	36.0	41.0	34.0	41.0
26-27	37.7131428216603	39.0	36.5	41.0	34.0	41.0
28-29	37.67763633786858	39.0	36.5	41.0	34.5	41.0
30-31	37.52207375015035	38.5	36.0	41.0	34.0	41.0
32-33	37.3813827904701	38.0	36.5	41.0	33.0	41.0
34-35	37.207688223614284	38.0	36.0	41.0	33.0	41.0
36-37	37.2261936630796	38.0	35.5	41.0	33.0	41.0
38-39	37.36731677130592	38.0	36.5	41.0	33.0	41.0
40-41	37.263688733771815	38.0	36.5	41.0	33.0	41.0
42-43	37.291514487725564	38.0	36.5	41.0	33.0	41.0
44-45	37.12598908633667	38.0	36.0	41.0	33.0	41.0
46-47	36.854879280400915	38.0	36.0	41.0	32.0	41.0
48-49	36.81412949848544	38.0	36.5	41.0	32.0	41.0
50-51	36.423282381238444	38.0	35.0	40.5	30.5	41.0
52-53	36.48122077524958	38.0	35.5	40.0	31.0	41.0
54-55	36.2897206161604	37.5	35.5	40.0	31.0	41.0
56-57	36.00090628904289	38.0	35.0	40.0	29.5	41.0
58-59	35.874907821525724	36.5	35.0	40.0	30.0	41.0
60-61	35.61592788195169	36.5	35.0	40.0	29.0	41.0
62-63	35.4392427846158	37.5	35.0	40.0	28.5	41.0
64-65	35.30145887407742	37.0	35.0	39.0	28.0	41.0
66-67	35.08306186542317	36.5	34.5	39.0	28.5	41.0
68-69	34.618616818647375	36.0	34.0	38.5	27.0	40.5
70-71	34.37765058832845	36.0	34.0	38.0	26.5	40.0
72-73	34.11209315690907	36.0	34.0	37.0	26.0	39.5
74-75	33.834389107019945	35.5	34.0	37.5	26.0	39.0
76-77	32.348675918967594	34.5	31.0	36.5	24.5	37.5
78-79	33.34258462913681	35.0	33.0	37.0	26.0	38.0
80-81	33.326897955204196	35.0	33.0	36.5	26.0	37.0
82-83	33.1404468256263	35.0	33.0	36.5	26.0	37.5
84-85	32.929772594601104	35.0	33.0	36.5	25.0	37.0
86-87	32.676354969127544	35.0	33.0	36.0	25.0	37.0
88-89	32.503546588785	35.0	33.0	36.0	25.0	36.5
90-91	32.35913130045385	35.0	32.5	35.5	24.5	37.0
92-93	32.204820821128024	35.0	32.5	35.5	24.0	36.5
94-95	32.07731460805037	35.0	32.0	35.0	24.0	36.0
96-97	31.897912597998435	35.0	32.0	35.0	23.0	36.5
98-99	31.765257345324713	35.0	32.0	35.0	22.5	36.5
100	30.58095752145638	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.11268466104544927
1101	2	-0.15903699048018183
1101	3	-0.1542956640286519
1101	4	-0.13180795127183842
1101	5	-0.07854609285170255
1101	6	-0.18395390136055312
1101	7	-0.1438761386891727
1101	8	-0.08754185873235087
1101	9	-0.14276851167012694
1101	10-11	-0.0964001153811509
1101	12-13	-0.14524458504282478
1101	14-15	-0.28343211333566387
1101	16-17	-0.15969989761436665
1101	18-19	-0.16795015287780046
1101	20-21	-0.21332140927103893
1101	22-23	-0.20288656740410715
1101	24-25	-0.2728899608019404
1101	26-27	-0.28041510781365275
1101	28-29	-0.35211327221522737
1101	30-31	-0.3071571243311553
1101	32-33	-0.257633341431486
1101	34-35	-0.24593760866500247
1101	36-37	-0.39051234401768653
1101	38-39	-0.2565956853052995
1101	40-41	-0.29575802405675233
1101	42-43	-0.3487101417694731
1101	44-45	-0.39236558667276
1101	46-47	-0.3971510936384277
1101	48-49	-0.4343233591550515
1101	50-51	-0.27409702531466706
1101	52-53	-0.4215022738830001
1101	54-55	-0.4276286781823586
1101	56-57	-0.428660030252324
1101	58-59	-0.507329829201467
1101	60-61	-0.5015491080150767
1101	62-63	-0.3828200376356463
1101	64-65	-0.4251798020339308
1101	66-67	-0.4234118990555231
1101	68-69	-0.40918176849428534
1101	70-71	-0.46799662100374206
1101	72-73	-0.5551180862143354
1101	74-75	-0.5090641808017651
1101	76-77	-0.4971259383150439
1101	78-79	-0.39114403546564347
1101	80-81	-0.28134476405867304
1101	82-83	-0.47946281092523435
1101	84-85	-0.5223495048680888
1101	86-87	-0.49787168963933226
1101	88-89	-0.5362423291537155
1101	90-91	-0.576345078362916
1101	92-93	-0.843281812239276
1101	94-95	-0.7748179631751171
1101	96-97	-0.6804919400865828
1101	98-99	-0.7231018171573105
1101	100	-0.7935519464903464
1102	1	-0.006572827300864503
1102	2	-0.03623007978207937
1102	3	-0.042581159311090744
1102	4	-0.04176782962153425
1102	5	-0.019248674223831586
1102	6	-0.07297965076066504
1102	7	-0.11797370873885171
1102	8	-0.029245851964311953
1102	9	-0.09192830868882851
1102	10-11	-0.09072139694044523
1102	12-13	-0.05121683281899436
1102	14-15	-0.1518560641584088
1102	16-17	-0.05266488981569495
1102	18-19	-0.05526852043276165
1102	20-21	-0.11445183471160902
1102	22-23	-0.08277152871418991
1102	24-25	-0.10147014042247804
1102	26-27	-0.08323589724139424
1102	28-29	-0.11397124140219006
1102	30-31	-0.12851873781320933
1102	32-33	-0.15477771227344306
1102	34-35	-0.15189483125655556
1102	36-37	-0.2565001314407809
1102	38-39	-0.21234336466887527
1102	40-41	-0.23341775846384394
1102	42-43	-0.22455105794620067
1102	44-45	-0.3062777162946162
1102	46-47	-0.2314130234480416
1102	48-49	-0.165556436122678
1102	50-51	-0.12484781976629478
1102	52-53	-0.11827707326406056
1102	54-55	-0.2876181715048247
1102	56-57	-0.15708888689800915
1102	58-59	-0.2760377199529529
1102	60-61	-0.24744022086278505
1102	62-63	-0.1938942344280079
1102	64-65	-0.33910942764853047
1102	66-67	-0.2935644406062323
1102	68-69	-0.21996466368522505
1102	70-71	-0.2412592528968176
1102	72-73	-0.3614798909502994
1102	74-75	-0.725093159935426
1102	76-77	-0.3079319121895452
1102	78-79	-0.2962112195435722
1102	80-81	-0.25572801295180625
1102	82-83	-0.27997311427530036
1102	84-85	-0.30536146486161186
1102	86-87	-0.28905705623674294
1102	88-89	-0.25106102632783944
1102	90-91	-0.3369972531121377
1102	92-93	-0.37293786719902755
1102	94-95	-0.38425701386765354
1102	96-97	-0.44184008138710595
1102	98-99	-0.6419433025013852
1102	100	-0.5048137083384177
1103	1	-0.15261831647459445
1103	2	-0.08653632561480862
1103	3	-0.11782814570277367
1103	4	-0.09963960152101947
1103	5	-0.1256011071615859
1103	6	-0.11930084091784465
1103	7	-0.08138503589456292
1103	8	-0.09438748697296262
1103	9	-0.15465659254100927
1103	10-11	-0.09474538765567786
1103	12-13	-0.10608280800557424
1103	14-15	-0.20510575627646688
1103	16-17	-0.16817905600887428
1103	18-19	-0.14093200845728404
1103	20-21	-0.16750163526790374
1103	22-23	-0.05931712811756995
1103	24-25	-0.10539903929296202
1103	26-27	-0.15789536967574946
1103	28-29	-0.1734060907582844
1103	30-31	-0.1956524411536762
1103	32-33	-0.10323560252654573
1103	34-35	-0.05048703186396608
1103	36-37	-0.16007916895003405
1103	38-39	-0.2668540317306949
1103	40-41	-0.2641665960302788
1103	42-43	-0.2856083386898902
1103	44-45	-0.37696619652437136
1103	46-47	-0.34445029722483866
1103	48-49	-0.36687267345286756
1103	50-51	-0.22969431578358268
1103	52-53	-0.2866431029700536
1103	54-55	-0.3178229281344116
1103	56-57	-0.18183880951979603
1103	58-59	-0.29611132167504195
1103	60-61	-0.3783582951191491
1103	62-63	-0.312822898756302
1103	64-65	-0.4058435290790712
1103	66-67	-0.3836866831692518
1103	68-69	-0.26477081740966213
1103	70-71	-0.40694391650894346
1103	72-73	-0.44168121875940614
1103	74-75	-0.34552133457274437
1103	76-77	-0.3486776900935169
1103	78-79	-0.3605312773227567
1103	80-81	-0.3425431329188413
1103	82-83	-0.40435524703735837
1103	84-85	-0.2824925201534114
1103	86-87	-0.4139169069208606
1103	88-89	-0.35808430559841753
1103	90-91	-0.34623747775075486
1103	92-93	-0.39214922865070534
1103	94-95	-0.13783233908655745
1103	96-97	-0.9012224215093241
1103	98-99	-1.2375161787994813
1103	100	-0.5185882600651226
1104	1	-0.03421224998130867
1104	2	-0.05936984631805586
1104	3	-0.09255658037422165
1104	4	-0.10674112089144927
1104	5	-0.21423693696252144
1104	6	-0.13552003285679604
1104	7	-0.13411244303227932
1104	8	-0.09289588700502804
1104	9	-0.06891884639610169
1104	10-11	-0.13030033588537293
1104	12-13	-0.10147646246375785
1104	14-15	-0.20882528339472373
1104	16-17	-0.06765298460438629
1104	18-19	-0.18276622424944833
1104	20-21	-0.2447611671576695
1104	22-23	-0.14902159098048173
1104	24-25	-0.1513904382669864
1104	26-27	-0.15459483088678638
1104	28-29	-0.21926802179545035
1104	30-31	-0.3024225286683446
1104	32-33	-0.21605178775027412
1104	34-35	-0.10938235693912901
1104	36-37	-0.23181363247869768
1104	38-39	-0.21424704284672202
1104	40-41	-0.1867776593556698
1104	42-43	-0.18105822748741218
1104	44-45	-0.24953583402538726
1104	46-47	-0.16894025032727455
1104	48-49	-0.24269449424835443
1104	50-51	-0.11221842156911066
1104	52-53	-0.22199975136048522
1104	54-55	-0.2628727470504444
1104	56-57	-0.23154530865613765
1104	58-59	-0.17920721207943302
1104	60-61	-0.18483112485704822
1104	62-63	-0.12083722486567439
1104	64-65	-0.31703314080574074
1104	66-67	-0.2721811295119778
1104	68-69	-0.16386161538748212
1104	70-71	-0.179221492380627
1104	72-73	-0.1850545361988125
1104	74-75	-0.2709512320961025
1104	76-77	-0.2588417384294033
1104	78-79	-0.2588374368301132
1104	80-81	-0.1605407372599572
1104	82-83	-0.19497254568914713
1104	84-85	-0.18234757113268074
1104	86-87	-0.1320376677869035
1104	88-89	-0.1541345906647038
1104	90-91	-0.1280136464882773
1104	92-93	-0.2636034816563253
1104	94-95	-0.32256670966808443
1104	96-97	-0.061269925967316397
1104	98-99	-0.9712803747241487
1104	100	-0.413119779694604
1105	1	-0.11719954860690507
1105	2	-1.0300466453006756
1105	3	-0.15376027712379425
1105	4	-0.08980533432220028
1105	5	-0.1391924261561499
1105	6	-0.1235519326068868
1105	7	-0.05454042320926078
1105	8	-0.10016405044341781
1105	9	-0.1905625007967373
1105	10-11	-0.08084606314246656
1105	12-13	-0.09091254921723646
1105	14-15	-0.1464025961126083
1105	16-17	-0.6719621669963018
1105	18-19	-0.1339722681299378
1105	20-21	-0.21098046949671811
1105	22-23	-0.17785105085953035
1105	24-25	-0.23739620502161074
1105	26-27	-0.19145993650090531
1105	28-29	-0.23350059859190964
1105	30-31	-0.16100564215265223
1105	32-33	-0.09728655773151473
1105	34-35	-0.09326601408401558
1105	36-37	-0.18029531846682545
1105	38-39	-0.18949450846405114
1105	40-41	-0.1600058374066009
1105	42-43	-0.21022811572184708
1105	44-45	-0.33462352917791094
1105	46-47	-0.228069461588305
1105	48-49	-0.2770419475796402
1105	50-51	-0.15780381716289327
1105	52-53	-0.21402923473998214
1105	54-55	-0.3442823604877745
1105	56-57	-0.18647076777332217
1105	58-59	-0.2938003468969299
1105	60-61	-0.26295619907973133
1105	62-63	-0.18452739958106434
1105	64-65	-0.19537275918623465
1105	66-67	-0.26242123116279714
1105	68-69	-0.2518118437472694
1105	70-71	-0.27858042504294644
1105	72-73	-0.24884489055097703
1105	74-75	-0.2362274701766296
1105	76-77	-0.1329421183874544
1105	78-79	-0.16363367580647292
1105	80-81	-0.11774756481793247
1105	82-83	-0.025519066934947432
1105	84-85	-0.04198064220104669
1105	86-87	-0.11803239369035623
1105	88-89	-0.06754624673549614
1105	90-91	-0.04060065890299569
1105	92-93	-0.06956311295523676
1105	94-95	-0.191711981972702
1105	96-97	-0.2013059080455193
1105	98-99	-0.13846983295594129
1105	100	-0.1610717277721001
1106	1	-0.08447571077056892
1106	2	-0.1252001913069236
1106	3	-0.0724857669171044
1106	4	-0.046662893498215396
1106	5	-0.08630096916181884
1106	6	-0.08149143825836092
1106	7	-0.06551858175660641
1106	8	-0.014431143887044584
1106	9	-0.11611988746495427
1106	10-11	-0.10758110223969197
1106	12-13	-0.07979504869292953
1106	14-15	-0.10908541433171592
1106	16-17	-0.009670532728399905
1106	18-19	-0.08958305016549417
1106	20-21	-0.07440411058302487
1106	22-23	-0.025141891404089733
1106	24-25	-0.00375594582709482
1106	26-27	-0.013940871591834991
1106	28-29	-0.10074201016003315
1106	30-31	-0.061498929667230584
1106	32-33	-0.010219488789587672
1106	34-35	0.02107436442528865
1106	36-37	-0.028731871723117308
1106	38-39	-0.0704863676899734
1106	40-41	-0.05108669552525669
1106	42-43	-0.011427058327946327
1106	44-45	-0.03591555891475906
1106	46-47	-0.10808381563708735
1106	48-49	-0.11053915977981887
1106	50-51	0.03494874530559855
1106	52-53	-0.07166588161251042
1106	54-55	-0.11343945300838953
1106	56-57	-0.041765056362486064
1106	58-59	-0.15251460945734863
1106	60-61	-0.0403292471298613
1106	62-63	-0.038025053636054906
1106	64-65	-0.04049207543615552
1106	66-67	0.024909702935424605
1106	68-69	0.037981248011888624
1106	70-71	0.005862846746609307
1106	72-73	-0.016788097022590875
1106	74-75	-0.05492577712359292
1106	76-77	-0.13525478438428706
1106	78-79	-0.0712167105815027
1106	80-81	0.09973040505079922
1106	82-83	0.00763599905690171
1106	84-85	0.12880168868936437
1106	86-87	0.05861452310114146
1106	88-89	0.06755817224114224
1106	90-91	0.0027036872375632015
1106	92-93	-0.006692790077025279
1106	94-95	-0.055891164244464164
1106	96-97	-0.08290514591848464
1106	98-99	0.025660077545058613
1106	100	0.0036078366201159895
1107	1	-0.02663520718200374
1107	2	-0.05533921951416687
1107	3	-0.053055234841636434
1107	4	-0.04706004736510039
1107	5	-0.03831299528359011
1107	6	-0.056236985119525684
1107	7	-0.03403725101394173
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2316	68-69	0.04052584488284339
2316	70-71	0.03675598256024415
2316	72-73	0.08582520192543797
2316	74-75	0.007964867909308282
2316	76-77	-0.11427336390495668
2316	78-79	-0.06391403519514682
2316	80-81	-0.003443977504339557
2316	82-83	-0.02245552244884408
2316	84-85	0.0954339387191041
2316	86-87	-0.11030099742943378
2316	88-89	-0.002036785102767169
2316	90-91	-0.06928724843537992
2316	92-93	-0.14367415768600722
2316	94-95	-0.07559423850820224
2316	96-97	0.09463745215967378
2316	98-99	0.14192153902827798
2316	100	-0.05570345663259957
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	8.0
12	35.0
13	179.0
14	614.0
15	1890.0
16	4125.0
17	7997.0
18	13704.0
19	20412.0
20	29710.0
21	42107.0
22	55420.0
23	62182.0
24	66342.0
25	71356.0
26	79944.0
27	93635.0
28	112955.0
29	138847.0
30	172548.0
31	216880.0
32	279372.0
33	374007.0
34	567861.0
35	1083188.0
36	1932962.0
37	1459405.0
38	1680016.0
39	386134.0
40	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.827132810384384	19.431918624448237	14.081437218867274	23.659511346300107
2	15.674713811069873	20.707490173685493	40.09620271217122	23.521593303073416
3	18.57028827587424	25.943991050075947	30.16419027456329	25.32153039948652
4	12.94050375833627	17.31375420966608	38.100734915142176	31.645007116855474
5	13.477607382997814	37.71974869717125	34.76659201083586	14.036051908995088
6	30.686136079126996	37.24330917172204	18.006980335404993	14.063574413745975
7	26.99958465940228	32.72422668639842	22.373568211289204	17.9026204429101
8	25.48009506302169	35.360071297266266	20.568434877586256	18.591398762125785
9	26.47931181439198	15.939855905051722	20.502616902923535	37.07821537763276
10-11	24.622459074732898	26.283536958032222	28.512634246529984	20.5813697207049
12-13	25.30598872827696	24.01879454389995	28.461731669378278	22.213485058444814
14-15	22.829275697174044	25.444768071437657	26.202471853964195	25.523484377424104
16-17	22.24014532060829	28.32036426198585	26.897775797283785	22.54171462012208
18-19	22.105165113808713	27.340375447777642	28.842042592531687	21.71241684588196
20-21	22.940532202496	26.485164907634772	28.88929987504011	21.685003014829118
22-23	22.762596211217808	26.343525596155654	28.592403393704384	22.30147479892215
24-25	22.474246077839076	26.557231332123337	28.691936234929894	22.276586355107693
26-27	22.316566101697944	26.72003065645559	28.710929995880903	22.25247324596556
28-29	22.216343615050782	26.738491034882067	28.780549397723853	22.2646159523433
30-31	22.67897937377512	26.559451243604304	28.66143959088546	22.100129791735114
32-33	22.45282906123093	26.744187020562283	28.546385024049613	22.25659889415717
34-35	22.69793777812822	26.799888068825943	28.518124289505923	21.984049863539905
36-37	22.673435810504998	26.670569307341218	28.439405919285477	22.216588962868308
38-39	22.523794260339695	26.835731931774525	28.446783726311843	22.193690081573937
40-41	22.64400916415735	26.65246524197864	28.490780419233342	22.212745174630673
42-43	22.462693413972616	26.67447216163908	28.339692818201932	22.523141606186368
44-45	22.420216726974708	26.871781572483204	28.268949007247407	22.43905269329468
46-47	22.505236423626126	26.75470526934204	28.03179335592048	22.708264951111353
48-49	22.488957657859526	26.889038619769227	27.974439806808444	22.647563915562806
50-51	22.51267991001965	27.062563977578748	27.777671976506678	22.647084135894925
52-53	22.55967473331195	27.06122032081363	27.69639862168431	22.682706324190114
54-55	22.39706244745007	26.97347197775471	27.85132138658493	22.778144188210288
56-57	22.436664901324363	27.09726351095536	27.59697972343169	22.869091864288585
58-59	22.399372202177435	26.98613711795992	27.786302365533082	22.828188314329566
60-61	22.61744294835261	27.097029375214227	27.729449920162146	22.556077756271016
62-63	22.372217800459346	27.030000995664476	27.858597104844474	22.739184099031704
64-65	22.320879868555583	26.80948695610268	27.837654327157914	23.03197884818383
66-67	22.284247717042234	26.953225122838287	27.62916378382695	23.133363376292532
68-69	22.22502002860799	27.035973758921568	27.621418933476207	23.117587278994236
70-71	22.312360709159748	26.841277558792733	27.59750980044084	23.24885193160668
72-73	22.20108488060461	27.084873680029915	27.317617739075583	23.396423700289894
74-75	22.196270554770503	27.15905785916316	27.277644147539103	23.367027438527234
76-77	22.16545410276228	27.037542944196	27.36810714939889	23.42889580364283
78-79	22.130892448921504	26.905346167439596	27.350871754670656	23.612889628968244
80-81	22.14703968334361	27.212770208460768	27.064937812002356	23.57525229619327
82-83	22.292559030446903	27.058137603821137	27.119976019605136	23.529327346126824
84-85	22.270795102107005	27.047563642828997	27.021363101585678	23.660278153478327
86-87	22.011200499710995	27.126358565533266	27.20950598982541	23.652934944930326
88-89	22.083109852951072	26.93977016397992	27.178736189470786	23.79838379359822
90-91	22.23055990013847	26.90243358874809	27.068392964327614	23.79861354678583
92-93	22.201881368859766	26.865081565463196	27.019882756050865	23.91315430962617
94-95	22.077154155530113	26.770639611632618	27.029295076745623	24.122911156091646
96-97	22.104572244280778	26.727096743462386	27.07360250002806	24.094728512228773
98-99	22.01786566381135	26.625255793161926	27.01750408417462	24.3393744588521
100	22.065484662719207	26.616979434435816	26.996535921608363	24.320999981236614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1765.0
1	1206.5
2	973.0
3	1313.0
4	1110.5
5	1068.5
6	1634.0
7	3207.5
8	5582.0
9	7123.0
10	6671.0
11	5870.0
12	6141.5
13	6797.5
14	7515.0
15	8214.0
16	9025.0
17	9890.0
18	10724.0
19	11864.5
20	13409.5
21	15197.5
22	17654.5
23	21489.5
24	26753.0
25	33259.0
26	42049.5
27	52773.5
28	64029.0
29	78081.5
30	95595.5
31	112501.0
32	131175.5
33	151980.5
34	173186.5
35	194325.0
36	219958.5
37	242848.5
38	256513.5
39	269358.5
40	277153.5
41	283021.5
42	290097.0
43	301509.0
44	313878.5
45	323406.5
46	330099.5
47	334557.0
48	342810.5
49	346684.0
50	343135.0
51	335755.5
52	326135.5
53	317043.0
54	304302.5
55	285759.5
56	261992.5
57	233410.0
58	206323.5
59	178262.5
60	148487.5
61	122405.0
62	99926.0
63	78291.5
64	59167.5
65	45063.5
66	33622.0
67	25594.5
68	20018.0
69	15473.0
70	12333.0
71	9675.0
72	7665.5
73	6260.0
74	4825.0
75	3528.5
76	2521.5
77	1713.5
78	1141.0
79	733.5
80	479.5
81	305.0
82	194.0
83	126.5
84	82.5
85	52.5
86	44.0
87	26.5
88	16.5
89	14.0
90	9.0
91	5.5
92	1.5
93	0.5
94	2.0
95	2.0
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03137111425082842
2	0.07759576426299604
3	0.0031047240162799215
4	2.233614400201383E-5
5	0.0013290005681198226
6	9.604541920865946E-4
7	0.0
8	4.132186640372558E-4
9	1.1168072001006915E-5
10-11	1.675210800151037E-5
12-13	1.0051264800906222E-4
14-15	0.011838156321067328
16-17	0.0011391433441027051
18-19	1.7310511601560717E-4
20-21	2.680337280241659E-4
22-23	0.0018371478441656375
24-25	8.096852200730013E-4
26-27	0.007605457032685709
28-29	0.00814152448873404
30-31	0.010135025340913774
32-33	0.009330924156841275
34-35	0.00424945139638313
36-37	0.0036910477963327847
38-39	0.0028701945042587766
40-41	0.0033560056363025775
42-43	0.0016584586921495267
44-45	0.0053774266684848285
46-47	0.01854458355767198
48-49	0.019421277209751023
50-51	0.013999178253262166
52-53	0.007879074796710378
54-55	0.0012564081001132778
56-57	0.0024011354802164864
58-59	0.0028925306482607905
60-61	0.00361845532832624
62-63	0.0030991399802794183
64-65	0.003914409236352923
66-67	0.0028143541442537424
68-69	0.007303919088658521
70-71	3.9646655603574544E-4
72-73	0.005416514920488353
74-75	0.007828818472705846
76-77	0.006890700424621266
78-79	0.004495148980405282
80-81	4.243867360382627E-4
82-83	0.001295496352116802
84-85	9.325340120840773E-4
86-87	0.00135692074812234
88-89	0.0011670635241052224
90-91	0.0011000550920991809
92-93	3.517942680317178E-4
94-95	0.0011279752721016982
96-97	0.0030991399802794183
98-99	0.0036798797243317778
100	0.005461187208492381
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8954097.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.14338330353006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03116460638306	36.51661859263239
2	16.517116206294805	17.225166428275564
3	6.028675616981635	9.430666305267568
4	2.7633517536328247	5.763620387686332
5	1.4451483890335	3.7677463189926375
6	0.8473966058926266	2.651167561870177
7	0.5161734147303254	1.884051975076274
8	0.34913169256740584	1.4563926135161966
9	0.2624560431748906	1.2316811453636536
>10	1.1268035394706695	10.09579806558932
>50	0.06455732676726078	2.3251548507250384
>100	0.043301243526989844	4.325626161757631
>500	0.003235332744012689	1.134781833059482
>1k	0.0013588175999508323	1.049523636512026
>5k	3.235279998128985E-5	0.09042236653966242
>10k+	9.705839994386953E-5	1.0515817571361674
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26637	0.2974839338908211	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22920	0.2559722102630784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12778	0.14270562402886633	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.350421600302074E-5
2	0.0	0.0	0.0	0.0	3.350421600302074E-5
3	0.0	0.0	0.0	0.0	3.350421600302074E-5
4	0.0	0.0	0.0	0.0	3.350421600302074E-5
5	0.0	0.0	0.0	0.0	3.350421600302074E-5
6	0.0	0.0	0.0	0.0	7.81765040070484E-5
7	0.0	0.0	0.0	0.0	7.81765040070484E-5
8	0.0	0.0	0.0	2.2336144002013826E-5	7.81765040070484E-5
9	0.0	0.0	0.0	5.5840360005034566E-5	7.81765040070484E-5
10-11	0.0	0.0	0.0	5.5840360005034566E-5	7.81765040070484E-5
12-13	0.0	0.0	0.0	8.376054000755186E-5	7.81765040070484E-5
14-15	0.0	0.0	0.0	8.93445760080553E-5	1.1726475601057259E-4
16-17	0.0	0.0	0.0	1.0609668400956567E-4	1.4518493601308987E-4
18-19	0.0	0.0	0.0	1.1726475601057259E-4	1.4518493601308987E-4
20-21	0.0	0.0	0.0	1.675210800151037E-4	1.4518493601308987E-4
22-23	5.5840360005034564E-6	0.0	0.0	4.5230691604078E-4	1.5076897201359333E-4
24-25	1.1168072001006913E-5	0.0	0.0	8.655255800780358E-4	1.6193704401460025E-4
26-27	1.1168072001006913E-5	0.0	0.0	0.0018427318801661407	1.8985722401711753E-4
28-29	1.1168072001006913E-5	0.0	0.0	0.004740846564427434	2.0102529601812445E-4
30-31	1.1168072001006913E-5	0.0	0.0	0.016020599285444418	2.0102529601812445E-4
32-33	1.1168072001006913E-5	0.0	0.0	0.04392402717996019	2.5686565602315903E-4
34-35	1.1168072001006913E-5	5.5840360005034564E-6	0.0	0.08827802513195915	2.847858360256763E-4
36-37	1.675210800151037E-5	1.1168072001006913E-5	0.0	0.1576429203302131	2.9036987202617974E-4
38-39	2.2336144002013826E-5	1.1168072001006913E-5	0.0	0.25492799553098433	2.959539080266832E-4
40-41	2.2336144002013826E-5	1.1168072001006913E-5	0.0	0.35866821634833757	3.127060160281936E-4
42-43	2.2336144002013826E-5	1.1168072001006913E-5	0.0	0.46337447539377785	3.127060160281936E-4
44-45	2.2336144002013826E-5	1.1168072001006913E-5	0.0	0.5973634192258583	3.127060160281936E-4
46-47	2.2336144002013826E-5	1.675210800151037E-5	0.0	0.7571896976322683	3.2945812402970396E-4
48-49	2.2336144002013826E-5	2.2336144002013826E-5	0.0	0.920930385275031	3.6296234003272467E-4
50-51	2.2336144002013826E-5	2.7920180002517283E-5	0.0	1.0921760173024706	3.6854637603322813E-4
52-53	2.2336144002013826E-5	3.350421600302074E-5	0.0	1.2659009613141337	4.076346280367523E-4
54-55	2.2336144002013826E-5	3.350421600302074E-5	0.0	1.4624646125678558	4.7464306004279383E-4
56-57	2.2336144002013826E-5	3.350421600302074E-5	0.0	1.691136470824473	5.081472760458146E-4
58-59	2.2336144002013826E-5	3.350421600302074E-5	0.0	1.9448136422913445	5.193153480468215E-4
60-61	2.2336144002013826E-5	3.350421600302074E-5	0.0	2.2086593433151327	5.360674560483318E-4
62-63	2.2336144002013826E-5	3.350421600302074E-5	0.0	2.476693071339299	5.584036000503456E-4
64-65	2.2336144002013826E-5	3.350421600302074E-5	0.0	2.755799942752463	5.639876360508491E-4
66-67	2.2336144002013826E-5	3.350421600302074E-5	0.0	3.0711248716648925	5.807397440523595E-4
68-69	2.2336144002013826E-5	3.350421600302074E-5	0.0	3.4203169789203756	5.919078160533664E-4
70-71	2.2336144002013826E-5	3.350421600302074E-5	0.0	3.796072345430254	6.254120320563872E-4
72-73	2.2336144002013826E-5	3.350421600302074E-5	0.0	4.172603892944203	7.370927520664563E-4
74-75	2.2336144002013826E-5	3.350421600302074E-5	0.0	4.554524035198636	7.370927520664563E-4
76-77	2.2336144002013826E-5	3.350421600302074E-5	0.0	4.956016223634834	7.426767880669597E-4
78-79	2.2336144002013826E-5	3.350421600302074E-5	0.0	5.404687932239287	7.482608240674632E-4
80-81	2.2336144002013826E-5	3.350421600302074E-5	0.0	5.877895895029951	7.873490760709874E-4
82-83	2.2336144002013826E-5	3.350421600302074E-5	0.0	6.368442289602179	8.487734720765254E-4
84-85	2.2336144002013826E-5	3.350421600302074E-5	0.0	6.85293000511386	8.599415440775323E-4
86-87	2.2336144002013826E-5	3.350421600302074E-5	0.0	7.3484573597985365	8.711096160785392E-4
88	2.2336144002013826E-5	3.350421600302074E-5	0.0	7.73779868589764	8.822776880795461E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15440	0.0	53.445568	1
GTATCAA	27650	0.0	40.63204	1
TCAACGC	33585	0.0	32.826115	4
ATCAACG	34115	0.0	32.34459	3
CAACGCA	34380	0.0	32.10805	5
TATCAAC	35350	0.0	31.357347	2
AACGCAG	35765	0.0	30.890429	6
ACGCAGA	41265	0.0	26.772757	7
CGCAGAG	42730	0.0	25.853525	8
GTACATG	34275	0.0	25.434744	1
TACATGG	35410	0.0	24.052582	2
ACATGGG	35235	0.0	23.540699	3
GCAGAGT	47440	0.0	23.019152	9
GTGGTAT	8095	0.0	22.549885	1
CATGGGG	22860	0.0	21.687677	4
TGGTATC	7975	0.0	21.465445	2
GAGTACT	27295	0.0	21.115465	12-13
CAGAGTA	46115	0.0	20.469797	10-11
GTACTTT	28615	0.0	19.932495	14-15
AGAGTAC	43260	0.0	19.71255	10-11
>>END_MODULE