FastQCFastQC Report
Mon 6 Jun 2016
ERR523051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105040
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222870.2749770513162181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182310.22493411506914218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109250.1347926722138324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG129950.053.2347642
GTACCTG153300.045.7218781
ACCTGGG149000.045.577513
CCTGGGG123650.034.9498524
TATCACG7750.030.3408152
CATGGGG228800.026.2868464
ATGGGGG149450.025.9254135
GTACATG403450.025.3017181
TGGGGGG155650.024.8770166
GTATCAA362350.024.588481
GTACACG16850.024.5676021
TACATGG419100.024.047142
CTGGGGG85800.023.6750495
ACATGGG421200.023.4030593
TATAACG7050.022.0132272
GGTATCA268650.021.6952381
TCAACGC411900.021.1204834
CAACGCA416300.020.8845375
ATCAACG418300.020.7995323
TATCAAC423100.020.574172
Produced by FastQC (version 0.11.5)