FastQCFastQC Report
Mon 6 Jun 2016
ERR523051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105040
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190330.23482919269985095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169060.20858626237501604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102580.12656322485761945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG129500.052.1145062
GTACCTG155300.043.7777061
ACCTGGG151050.043.6486363
CCTGGGG128500.034.1933944
TATAACG7050.032.664872
CATGGGG224500.024.3652234
GTACATG402500.024.0283851
TGGGGGG141300.023.9455096
ATGGGGG140800.023.7301625
CTGGGGG87500.023.4696775
GTATCAA371350.023.0939161
TACATGG414350.023.0364992
ACATGGG411050.022.5676463
TAACGCA9450.022.377654
GTACACG17950.022.0024491
GGTATCA276600.021.8937361
TCAACGC419150.020.1022454
CAACGCA422000.019.9108055
ATCAACG427300.019.7078323
TATCAAC432000.019.5822562
Produced by FastQC (version 0.11.5)