FastQCFastQC Report
Mon 6 Jun 2016
ERR523050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10621040
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT853640.8037254355505676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311420.29321045773295273No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT270060.2542688851562559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226500.21325595233611774No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC189700.17860774462764475No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC184620.17382478552006206No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG150340.14154922681771276No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA141600.13332027748695044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136060.12810421578301184No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA122080.11494166296332563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG387100.026.270841
TACATGG394000.025.2476542
ACATGGG399350.024.1804713
CATGGGG261900.021.3239754
GAGTACT278000.020.0521912-13
ATGGGGG154500.018.9561845
AGAGTAC456650.018.90365610-11
GTACTTT293600.018.61843114-15
AGTACTT290500.017.91977312-13
GAGTACA305250.016.8808961
AGTACAT296600.015.9926942
CATGGGA139100.015.9177264
GTGTAGC61000.015.8002741
TACCGTA6450.015.2962197
ACTTTTT362850.015.13541916-17
CATGGGT72650.015.0767424
TACTTTT338000.015.04679214-15
TATACTG47850.015.0314475
GTATATA52150.014.8754541
TACACTG111500.014.2084385
Produced by FastQC (version 0.11.5)