FastQCFastQC Report
Mon 6 Jun 2016
ERR523050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10621040
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT870810.8198914607232436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285290.26860834720517013No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT266550.2509641240405836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220120.2072490076301379No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC191680.18047196884674194No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC191310.18012360371489045No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA146570.13799966858236104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129850.12225733073220701No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC112380.10580884734451618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG385750.026.0321161
TACATGG396150.024.64442
ACATGGG401600.023.6259863
CATGGGG266100.021.3161954
GAGTACT291050.019.95723512-13
AGAGTAC472700.019.52355610-11
GTACTTT303200.018.9138614-15
ATGGGGG153050.018.791585
AGTACTT303750.018.5423912-13
GAGTACA309650.017.2705781
AGTACAT297000.016.6022
TACTTTT356300.015.75868214-15
ACTTTTT372500.015.56243516-17
CATGGGA139600.015.0813394
GTATAGG30100.015.0010711
GTATATA51300.014.9447181
GTGTAGC57450.014.1635981
TATACTG46200.013.9355415
GTATAGC40400.013.6214081
TACACTG116350.013.53080755
Produced by FastQC (version 0.11.5)