FastQCFastQC Report
Mon 6 Jun 2016
ERR523049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9329637
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257760.27628084565348043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233090.2498382305763879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111860.11989748368559248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164750.051.442441
GTATCAA280200.038.7279431
TCAACGC340650.031.3729324
ATCAACG346000.030.8631523
CAACGCA348400.030.6067855
TATCAAC353000.030.562
AACGCAG361800.029.6341066
ACGCAGA410100.026.0751747
GTGGTAT83750.025.2286971
CGCAGAG429000.024.9139868
GTACATG323350.022.9941581
TGGTATC89850.022.861712
GCAGAGT472300.022.1911479
TACATGG333650.021.963442
ACATGGG339850.020.9938723
GAGTACT256100.020.85820812-13
CAGAGTA453500.020.40720610-11
TACCTGG77300.019.9452612
CATGGGG239050.018.9522744
AGAGTAC421050.018.87265210-11
Produced by FastQC (version 0.11.5)