FastQCFastQC Report
Mon 6 Jun 2016
ERR523049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9329637
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230580.24714787938694718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225220.24140274696646827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106960.11464540367433373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164950.051.971471
GTATCAA274600.037.781311
TCAACGC334350.030.5840844
ATCAACG338650.030.182353
CAACGCA340900.029.9964475
TATCAAC347450.029.6329982
AACGCAG356750.028.7424686
ACGCAGA414150.024.7700587
GTGGTAT82300.024.411641
CGCAGAG431200.023.7948368
GTACATG322350.022.025741
TACCTGG74100.021.4546132
GCAGAGT476950.021.2659119
TGGTATC90500.021.1008342
TACATGG333700.020.9029252
GAGTACT258950.020.27491212-13
ACATGGG341450.019.9428023
CAGAGTA464100.019.68310210-11
AGAGTAC427400.018.64042310-11
GTACTTT281000.018.47322514-15
Produced by FastQC (version 0.11.5)