FastQCFastQC Report
Mon 6 Jun 2016
ERR523048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5363836
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194210.362072964199502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155060.28908415544397703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97750.18223897971526348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101850.043.6114041
TACCTGG55350.038.046542
GTATCAA166550.036.6141931
GTACCTG68700.030.683891
TCAACGC202600.029.8094224
GTACATG179000.029.3096711
CAACGCA206250.029.2590965
ATCAACG208550.028.9385723
ACCTGGG70950.028.817693
TATCAAC210450.028.7464582
AACGCAG214600.028.331446
TACATGG183900.027.8611832
ACATGGG187400.026.8371543
ACGCAGA243450.024.9737937
CATGGGG138850.024.201934
CGCAGAG253950.023.9037598
ATGGGGG90100.023.4213225
GCAGAGT279600.021.2233549
CCTGGGG69750.020.8885024
GAGTACT162700.020.24533712-13
Produced by FastQC (version 0.11.5)