FastQCFastQC Report
Mon 6 Jun 2016
ERR523048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5363836
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177530.33097581656113273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154550.2881333433758974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98230.18313386166169135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110850.043.7089461
TACCTGG61050.036.2052542
GTATCAA180900.034.7925571
GTACCTG73800.031.2963681
ACCTGGG70700.030.4439983
TCAACGC217700.028.343794
ATCAACG221200.027.938063
CAACGCA221950.027.8222225
TATCAAC226700.027.466032
AACGCAG231650.027.04146
GTACATG175500.026.267441
TACATGG181700.025.054162
ACGCAGA262350.023.8412137
ACATGGG190200.023.0776733
CGCAGAG271750.023.0039568
CATGGGG137450.021.950374
TATCACG5300.021.295842
CCTGGGG69800.021.006364
GCAGAGT297700.020.8248739
ATGGGGG85750.020.332535
Produced by FastQC (version 0.11.5)