FastQCFastQC Report
Mon 6 Jun 2016
ERR523047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7412760
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255320.3444331126328115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202210.272786384558518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109250.14738100248760244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98500.043.8757822
ACCTGGG109250.038.6542783
GGTATCA166700.038.4226341
GTATCAA265700.034.9028551
GTACCTG135950.032.654151
TATAACG6450.030.6221162
TCAACGC312600.029.304274
CAACGCA317650.028.8662435
ATCAACG317400.028.7878043
TATCAAC319700.028.7574082
AACGCAG327200.028.1276746
CCTGGGG106000.026.0856934
ATAACGC8550.025.3008083
GTACATG282500.024.9366991
ACGCAGA371550.024.7692187
CGCAGAG378650.024.2667148
TACATGG287200.024.0046372
ACATGGG287050.023.0995883
CATGGGG177350.022.8563564
TAACGCA9750.021.703954
Produced by FastQC (version 0.11.5)