FastQCFastQC Report
Mon 6 Jun 2016
ERR523047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7412760
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241640.32597844797349435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198080.2672149105056686No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT120160.16209886735844678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.14731355122788276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98750.044.5563472
GGTATCA166600.039.5243031
ACCTGGG112150.038.434943
GTATCAA261950.033.0557141
GTACCTG137250.032.966581
CCTGGGG101350.028.152774
ATAACGC8100.027.855593
TCAACGC309700.027.5025624
CAACGCA315000.027.0552925
ATCAACG316650.026.8989223
TATCAAC319800.026.7377642
TATAACG8400.026.3020382
AACGCAG327800.026.1234876
TAACGCA8650.026.0844274
GTACATG273550.023.4696771
ACGCAGA369650.023.153517
TACATGG279800.022.5631182
CGCAGAG380500.022.5295818
ACATGGG275550.021.7503343
TATCACG7150.021.6959532
Produced by FastQC (version 0.11.5)