FastQCFastQC Report
Mon 6 Jun 2016
ERR523046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7875970
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283240.35962554453610157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233400.2963444502708873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147160.1868468264861344No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117420.14908639824681913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91780.11653167800283648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG112300.049.867292
GTACCTG132550.042.686861
ACCTGGG133900.040.4531973
CCTGGGG122850.028.7796134
TATAACG6800.026.9674222
GTACATG333200.022.7545551
TATCACG7100.022.5166782
CATGGGG187050.022.4458484
TACATGG339200.021.9713732
ATGGGGG111350.021.7862435
TAACGCA10250.021.0996954
ACATGGG346750.021.0453553
GTATCAA450700.020.7461321
ATAACGC9550.020.6788753
GAGTACT247600.020.06900212-13
GGTATCA349600.019.9785211
TGGGGGG117500.018.9963936
CTGGGGG81550.018.8515345
AGTACTT263300.018.36371612-13
TCAACGC504350.018.1779464
Produced by FastQC (version 0.11.5)