FastQCFastQC Report
Mon 6 Jun 2016
ERR523046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7875970
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258170.3277945446719579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231240.2936019309367608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145210.1843709409761591No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT126130.16014535352470868No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82860.10520608890079572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123400.049.9696772
GTACCTG141600.044.3318141
ACCTGGG140700.043.1851463
CCTGGGG121650.032.371434
TATAACG6800.029.0287462
CTGGGGG80900.023.1768475
GTACATG338550.021.9448071
GTACACG15050.021.8705181
CATGGGG195850.021.5947864
TACATGG346500.021.0511762
GTATCAA454350.020.9467891
TGGGGGG110450.020.9328986
ATAACGC9700.020.8317953
ATGGGGG113750.020.6148015
ACATGGG347100.020.2267463
GAGTACT259250.020.16396712-13
GGTATCA354550.020.10571
TAACGCA9500.019.786384
AGTACTT274800.018.29590812-13
TCAACGC510800.018.2800334
Produced by FastQC (version 0.11.5)