FastQCFastQC Report
Mon 6 Jun 2016
ERR523045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331288
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246470.29583661013759216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206860.2482929410194438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111520.1338568538262031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153350.044.602941
GTATCAA260950.038.1993981
TCAACGC306750.032.095814
CAACGCA312950.031.413765
ATCAACG314350.031.2630423
TATCAAC325800.030.4407982
AACGCAG324750.030.3683176
GTACATG303850.026.8146131
ACGCAGA368750.026.6298717
TACATGG310600.025.7108612
CGCAGAG383200.025.587378
ACATGGG318050.024.633773
GCAGAGT422450.022.7080769
CATGGGG212200.021.7256374
GAGTACT219500.021.38165712-13
AGAGTAC357800.020.2323610-11
CAGAGTA406150.019.7496110-11
GTACTTT237150.019.60312314-15
ATGGGGG115900.018.3674135
AGTACTT240650.017.84311912-13
Produced by FastQC (version 0.11.5)