FastQCFastQC Report
Mon 6 Jun 2016
ERR523045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331288
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228610.27439934857611453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198440.2381864604848614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112280.1347690777224362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154000.046.5259861
GTATCAA259850.036.825161
TCAACGC313100.029.972734
CAACGCA318600.029.5143135
ATCAACG319350.029.417333
TATCAAC328800.028.6644782
AACGCAG330250.028.4724736
GTACATG296250.025.3449211
ACGCAGA378350.024.815337
TACATGG304750.024.1054632
CGCAGAG392300.023.9152688
ACATGGG308900.023.0337873
GCAGAGT433300.021.4893659
GAGTACT233500.020.9858912-13
CATGGGG210200.020.2323914
AGAGTAC368400.019.8849910-11
GTACTTT251500.019.43462814-15
CAGAGTA421400.019.15757210-11
AGTACTT254600.018.0466512-13
GAGTACA233800.017.506171
Produced by FastQC (version 0.11.5)